The basidiomycetous mushroom Lentinula edodes white collar-2 homolog PHRB, a partner of putative blue-light photoreceptor PHRA, binds to a specific site in the promoter region of the L. edodes tyrosinase gene

Regulation of fruiting body photomorphogenesis in Coprinopsis cinerea

“…These suggest that the C. cinerea genome has a wc-2 homolog and that Dst1 forms a complex with a presumptive WC-2 homolog to act as a blue-light photoreceptor. In fact, we found that the C. cinerea genome has a wc-2 homolog encoding a protein with a zinc finger DNA-binding domain and a PAS domain like those from other fungi such as L. edodes (Sano et al, 2009), N. crassa and P. blakesleeanus (Idnurm and Heitman, 2005) and disruption of the wc-2 homolog causes blind phenotypes in both morphogenesis of the fruiting body primordium as well as oidia production (unpublished results). These findings strongly suggest that the central mechanism for blue-light perception in agaricomycetous mushrooms is fundamentally similar to that in N. crassa.…”

“…Searches on the same genome databases also suggest that each of S. commune and L. bicolor has a wc-2 homolog. Furthermore, it has been shown in L. edodes that the WC-1 homolog PHRA interacts with the WC-2 homolog PHRB in vitro (Sano et al, 2009). These suggest that the C. cinerea genome has a wc-2 homolog and that Dst1 forms a complex with a presumptive WC-2 homolog to act as a blue-light photoreceptor.…”

Senescence of the Lentinula edodes Fruiting Body After Harvesting

“…On the other hand, Tyr is involved in browning of the surface of vegetative mycelia (Sano et al, 2010), suggesting that LeTyr is involved in melanin synthesis in different tissues. Browning of the surface of vegetative mycelia is regulated by light, and the blue light receptor PHRB regulates Letyr (Sano et al, 2009). This suggests that melanin synthesis in vegetative mycelium under light is caused by an increase of LeTyr via PHRB.…”

“…In N. crassa, WC2 acts as a component of the blue light receptor by interacting with white collar protein 1 (WC1). WC2 in L. edodes was cloned and characterized; the protein has been designated PHRB; Sano et al, 2009). Genes similar to WC1 have also been reported in L. edodes (PHRA; Sano et al, 2008) and in C. cinerea (dst1; Terashima et al, 2005).…”

Identification of Blue-Light Photoresponse Genes in Oyster Mushroom Mycelia

“…3,4) Genes encoding for blue photoreceptors found in the Basidiomycota mushrooms, dst1 in Coprinus cinereus 5) and phrA and phrB in Lentinula edodes, 6,7) are thought to be the homologue genes of wc-1 or wc-2 in Ascomycota Neurospora crassa. Therefore, blue-light receptors are widely conserved by plants and organisms, and it is likely that blue light plays an important role in biocontrol systems.…”