The Basis of Tolerance Mechanism to Metsulfuron-Methyl in Roegneria kamoji (Triticeae: Poaceae)

Tang, Wei; Liu, Shengnan; Yu, Xiaoyue; Yang, Yongjie; Zhao, Xiaogang; Lu, Yongliang

doi:10.3390/plants10091823

Cited by 2 publications

(4 citation statements)

References 37 publications

(54 reference statements)

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“…Mesosulfuron‐methyl resistance has evolved in 27 weed populations, bispyribac‐sodium resistance in 14 weed populations, and pyriminobac‐methyl resistance in two weed populations (Heap, 2023). Although metabolic resistance to mesosulfuron‐methyl and/or bispyribac‐sodium has been implicated in a few weedy species (Huang et al., 2019; Iwakami, Uchino, et al., 2014; Riar et al., 2013; Tang et al., 2021; Yu et al., 2021; Zhao et al., 2017), the only gene identified to be involved in mesosulfuron‐methyl resistance is CYP709C56 in A. aequalis CYP709C56 (Zhao et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

Metabolic resistance to acetolactate synthase inhibitors in Beckmannia syzigachne: identification of CYP81Q32 and its transcription regulation

Wang

Lian

et al. 2023

The Plant Journal

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SUMMARY Frequent herbicide use selects for herbicide resistance in weeds. Cytochrome P450s are important detoxification enzymes responsible for herbicide resistance in plants. We identified and characterized a candidate P450 gene (BsCYP81Q32) from the problematic weed Beckmannia syzigachne to test whether it conferred metabolic resistance to the acetolactate synthase‐inhibiting herbicides mesosulfuron‐methyl, bispyribac‐sodium, and pyriminobac‐methyl. Transgenic rice overexpressing BsCYP81Q32 was resistant to the three herbicides. Equally, rice overexpressing the rice ortholog gene OsCYP81Q32 was more resistant to mesosulfuron‐methyl. Conversely, an OsCYP81Q32 gene knockout generated using CRISPR/Cas9 enhanced mesosulfuron‐methyl sensitivity in rice. Overexpression of the BsCYP81Q32 gene resulted in enhanced mesosulfuron‐methyl metabolism in transgenic rice seedlings via O‐demethylation. The major metabolite, demethylated mesosulfuron‐methyl, was chemically synthesized and displayed reduced herbicidal effect in plants. Moreover, a transcription factor (BsTGAL6) was identified and shown to bind a key region in the BsCYP81Q32 promoter for gene activation. Inhibition of BsTGAL6 expression by salicylic acid treatment in B. syzigachne plants reduced BsCYP81Q32 expression and consequently changed the whole plant response to mesosulfuron‐methyl. Sequence polymorphisms in an important region of the BsTGAL6 promoter may explain the higher expression of BsTGAL6 in resistant versus susceptible B. syzigachne plants. Collectively, the present study reveals the evolution of an herbicide‐metabolizing and resistance‐endowing P450 and its transcription regulation in an economically important weedy plant species.

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Section: Introductionmentioning

confidence: 99%

Metabolic resistance to acetolactate synthase inhibitors in Beckmannia syzigachne: identification of CYP81Q32 and its transcription regulation

Wang

Lian

et al. 2023

The Plant Journal

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“…), an annual grass weed of the family Triticeae, is tolerant to most herbicides in wheat, and mesosulfuron-methyl is the only wheat-registered herbicide that provides effective control of this weed in China [29]. In our previous studies, R. kamoji showed a response pattern to all foliar-applied AC-Case-and ALS-inhibiting herbicides that was similar to that shown by wheat [3,4]. The results of this study indicated that R. kamoji also exhibited a similar response to the PRE herbicides chlorotoluron and diflufenican, and only buatchlor provides relatively higher control for R. kamoji while having no impact on the seedling emergence of wheat.…”

Section: Discussionmentioning

confidence: 84%

“…The rhizome of this population was separately cultivated in a greenhouse at China National Rice Research Institute (CNRRI, 30.04 N, 119.55 E), and seeds collected in 2020 were used in this study. Details of this population can be found in our previous studies [3,4]. Wheat seeds of the variety Yangmai 25 were purchased from China National Seed Group Co. Ltd. (Beijing, China).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…R. kamoji has been found to be highly tolerant to most post-emergence sprayed herbicides in wheat, including acetyl-CoA carboxylases (EC 6.4.1.2, ACCase) inhibitors (e.g., fenoxaprop-ethyl) and acetolactate synthase (EC 4.1.3.18, ALS) inhibitors (e.g., metsulfuron-methyl). The tolerance mechanism of R. kamoji to ACCase and ALS inhibitors was conferred by non-target-site mutation and GST/P450s-involved enhanced metabolism [3,4]. As a result, R. kamoji has become a dominant and problematic weed in wheat fields in the Middle-Lower Yangtze River region in recent years [5].…”

Section: Introductionmentioning

confidence: 99%

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Fenclorim Increasing Butachlor Selectivity between Wheat and Roegneria kamoji by Seed Soaking

Tang

Sun

et al. 2022

Agronomy

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Roegneria kamoji Ohwi (Poaceae), a wild relative plant of wheat which is widely distributed across China, has become a dominant and problematic weed in wheat fields in some regions. We have previously confirmed that R. kamoji is highly tolerant to foliar-applied acetyl-CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors in wheat (Triticum aestivum L.). The sensitivity of R. kamoji to pre-emergence (PRE) herbicides and the basis of fenclorim increase selectivity to butachlor between wheat and R. kamoji were evaluated in this study. Screenhouse bioassay showed that R. kamoji exhibited similar sensitivity to wheat to PRE herbicides at their recommended field doses (RFD); it also showed that buatchlor provides the highest relative control for R. kamoji (53.4% emergence and 81.5% fresh weight reduction, respectively), while it had no impact on seedling emergence of wheat among the six PRE herbicides. When butachlor was applied at four-fold RFD, no R. kamoji seedlings emerged; however, it significantly reduced the above-ground biomass of wheat compared with the non-treated control. Pre-treatment with herbicide safener fenclorim by seed soaking increased the ED10 value of butachlor to wheat from 221.8 to 1600.1 g a.i. ha−1, thus increasing the selectivity index from 9.6 to 68.9 between wheat and R. kamoji. The activities of α-amylase activity and protein content during germination, and glutathione-S-transferase (GST) and β-ketoacyl-CoA synthase (KCS) in the seedlings, could be induced by butachlor in both wheat seeds with or without fenclorim pre-soaking. These results suggested that butachlor provides the highest control for R. kamoji and did not affect germination and emergence in wheat. The basis of fenclorim-increased selectivity to butachlor was associated with the induced GST and KCS-mediated enhanced herbicide metabolism in wheat.

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The Basis of Tolerance Mechanism to Metsulfuron-Methyl in Roegneria kamoji (Triticeae: Poaceae)

Cited by 2 publications

References 37 publications

Metabolic resistance to acetolactate synthase inhibitors in Beckmannia syzigachne: identification of CYP81Q32 and its transcription regulation

Metabolic resistance to acetolactate synthase inhibitors in Beckmannia syzigachne: identification of CYP81Q32 and its transcription regulation

Fenclorim Increasing Butachlor Selectivity between Wheat and Roegneria kamoji by Seed Soaking

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