The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic

Carvajal-Oliveros, Angel; Uriostegui-Arcos, Maritere; Zurita, Mario; Melchy-Perez, Erika I.; Narváez-Padilla, Verónica; Reynaud, Enrique

doi:10.1016/j.ibneur.2022.11.007

Cited by 5 publications

(6 citation statements)

References 30 publications

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“…SH-SY5Y cells instead mildly express TH only when differentiated with staurosporine and present an increase in dopamine-ß-hydroxylase [236], which converts dopamine to noradrenaline and is specific of noradrenergic neurons [235,236]. Moreover, differentiation induces an increase in VMAT2 and SERT, a specific serotonin transporter, as it would be expected by serotonergic neurons [233]. Therefore, as it has been reported in different papers [233,235,236], the use of the BE(2)-M17 cell line could be more suitable to characterize the molecular mechanisms of PD that directly involve dopamine metabolism.…”

Section: Contribution Of Genes Located On the Sex Chromosomes To Park...mentioning

confidence: 98%

“…The main cellular lines used to study PD are the well-established human neuroblastoma cell line SH-SY5Y and the lesser-characterized BE(2)-M17 [233].…”

Section: Contribution Of Genes Located On the Sex Chromosomes To Park...mentioning

confidence: 99%

“…They can both differentiate into neuron-like cells upon differentiation protocols with the administration of retinoic acid (RA). However, it has been reported that while RA is suited for obtaining valuable BE(2)-M17 neuronal morphology and functionality, in the SH-SY5Y cell line the most evident effects are induced after treatment with staurosporine [233,235].…”

Section: Contribution Of Genes Located On the Sex Chromosomes To Park...mentioning

confidence: 99%

“…It has been observed that these two cell lines show different features at the catecholaminergic level, with the BE(2)-M17 cell line having a more prominent dopaminergic phenotype, compared with the SH-SY5Y cell line, which expresses a more noradrenergic phenotype [233,235]. BE(2)-M17 cells show a more pronounced expression of TH and other markers associated with dopamine metabolism, such as DAT, a specific dopamine transporter, and VMAT2, a monoamine transporter that loads dopamine, serotonin, and other neurotransmitters into synaptic vesicles.…”

Section: Contribution Of Genes Located On the Sex Chromosomes To Park...mentioning

confidence: 99%

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Sex and Brain: The Role of Sex Chromosomes and Hormones in Brain Development and Parkinson’s Disease

Terrin

Tesoriere

Plotegher

et al. 2023

Cells

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Sex hormones and genes on the sex chromosomes are not only key factors in the regulation of sexual differentiation and reproduction but they are also deeply involved in brain homeostasis. Their action is crucial for the development of the brain, which presents different characteristics depending on the sex of individuals. The role of these players in the brain is fundamental in the maintenance of brain function during adulthood as well, thus being important also with respect to age-related neurodegenerative diseases. In this review, we explore the role of biological sex in the development of the brain and analyze its impact on the predisposition toward and the progression of neurodegenerative diseases. In particular, we focus on Parkinson’s disease, a neurodegenerative disorder that has a higher incidence in the male population. We report how sex hormones and genes encoded by the sex chromosomes could protect from the disease or alternatively predispose toward its development. We finally underline the importance of considering sex when studying brain physiology and pathology in cellular and animal models in order to better understand disease etiology and develop novel tailored therapeutic strategies.

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Section: Contribution Of Genes Located On the Sex Chromosomes To Park...mentioning

confidence: 98%

“…The main cellular lines used to study PD are the well-established human neuroblastoma cell line SH-SY5Y and the lesser-characterized BE(2)-M17 [233].…”

Section: Contribution Of Genes Located On the Sex Chromosomes To Park...mentioning

confidence: 99%

Section: Contribution Of Genes Located On the Sex Chromosomes To Park...mentioning

confidence: 99%

Section: Contribution Of Genes Located On the Sex Chromosomes To Park...mentioning

confidence: 99%

See 2 more Smart Citations

Sex and Brain: The Role of Sex Chromosomes and Hormones in Brain Development and Parkinson’s Disease

Terrin

Tesoriere

Plotegher

et al. 2023

Cells

View full text Add to dashboard Cite

show abstract

“…The human neuroblastoma SH-SY5Y cell line is frequently used to model PD in vitro ( Krishna et al, 2014 ) due to its ability to differentiate to the dopaminergic phenotype ( Xicoy et al, 2017 ). Other neuroblastoma cell lines have been established with different properties than SH-SY5Y cells, however, this remains one of the most widely used cell lines to model PD in vitro ( Carvajal-Oliveros et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Protective effects of amphetamine and methylphenidate against dopaminergic neurotoxicants in SH-SY5Y cells

Carneiro,

Ferreira,

Marisa Costa

et al. 2024

Current Research in Toxicology

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PTPRJ is a negative regulator of insulin signaling in neuronal cells, impacting protein biosynthesis, and neurite outgrowth

Ulke,

Chopra,

Kadiri

et al. 2024

J Neuroendocrinology

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Central insulin resistance has been linked to the development of neurodegenerative diseases and mood disorders. Various proteins belonging to the enzyme family of protein tyrosine phosphatases (PTPs) act as inhibitors of insulin signaling. Protein tyrosine phosphatase receptor type J (PTPRJ) has been identified as a negative regulator in insulin signaling in the periphery. However, the impact of PTPRJ on insulin signaling and its functional role in neuronal cells is largely unknown. Therefore, we generated a Ptprj knockout (KO) cell model in the murine neuroblast cell line Neuro2a by CRISPR‐Cas9 gene editing. Ptprj KO cells displayed enhanced insulin signaling, as shown by increased phosphorylation of the insulin receptor (INSR), IRS‐1, AKT, and ERK1/2. Further, proximity ligation assays (PLA) revealed both direct interaction of PTPRJ with the INSR and recruitment of this phosphatase to the receptor upon insulin stimulation. By RNA sequencing gene expression analysis, we identified multiple gene clusters responsible for glucose uptake and metabolism, and genes involved in the synthesis of various lipids being mainly upregulated under PTPRJ deficiency. Furthermore, multiple Ca2+ transporters were differentially expressed along with decreased protein biosynthesis. This was accompanied by an increase in endoplasmic reticulum (ER) stress markers. On a functional level, PTPRJ deficiency compromised cell differentiation and neurite outgrowth, suggesting a role in nervous system development. Taken together, PTPRJ emerges as a negative regulator of central insulin signaling, impacting neuronal metabolism and neurite outgrowth.

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The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic

Cited by 5 publications

References 30 publications

Sex and Brain: The Role of Sex Chromosomes and Hormones in Brain Development and Parkinson’s Disease

Sex and Brain: The Role of Sex Chromosomes and Hormones in Brain Development and Parkinson’s Disease

Protective effects of amphetamine and methylphenidate against dopaminergic neurotoxicants in SH-SY5Y cells

PTPRJ is a negative regulator of insulin signaling in neuronal cells, impacting protein biosynthesis, and neurite outgrowth

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