The Behavior of Spindle Fibers and Movement of Chromosomes in Dividing Grasshopper Spermatocytes

Izutsu, Kosaku; Sato, Hidemi; Nakabayashi, Hiroshi; Aoki, Natsue

doi:10.1247/csf.2.119

Cited by 16 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies of animal cells have revealed a biphasic migration in which the rate is fast initially, then changes to a slower rate for a considerable portion of anaphase A [Hughes and Swann, 1948;Izutsu et al, 1977;Mazia, 1961;Sakai et al, 19791. We found just the opposite in Fusarium, where chromosomes typically are accelerated during the final few seconds of migration as they closely approach the spindle pole.…”

Section: Migration Of the Mamentioning

confidence: 99%

Video motion analysis of mitotic events in living cells of the fungus fusarium solani

Aist¹,

Bayles²

1988

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

An earlier, laser microbeam study produced evidence that, in Fusarium solani, extranuclear polar forces function at anaphase B of mitosis to pull apart the incipient daughter nuclei, whereas the central spindle functions primarily to limit the rate at which they separate. To elucidate further the various dynamics of mitotic anaphase, 8-14 mitoses in hyphae of F . solani were analyzed at 0.5 -2.0-sec intervals using high-resolution, digitally processed, videotaped sequences. The spindle growth rate, although fluctuating frequently, averaged 0.6 prdmin during metaphase, increased to 3.6 p d m i n during anaphase A and was maximal at 6.1 p d r n i n during anaphase B. Commonly, chromosomes migrated poleward during anaphase A at fluctuating rates, the average rate being an unprecedented 7.5 p d m i n . During anaphase the mitotic apparatus migrated to and fro in the hyphae at rates of 3-15 p d m i n , an apparent effect of opposing, fluctuating and typically unequal cytoplasmic forces applied to the two spindle poles. Thus, the molecular mechanisms underlying the various anaphase movements in F. soluni do not operate entirely smoothly and uniformly. Accelerated growth of the central spindle is temporally associated with anaphase A and the development of asters. Thus, chromosome disjunction may allow the polar forces to increase the rate of spindle elongation. Microtubule dynamics and motor molecules appear to be adequate to account for the observed rates of mitotic movements.

show abstract

Section: Migration Of the Mamentioning

confidence: 99%

Video motion analysis of mitotic events in living cells of the fungus fusarium solani

Aist¹,

Bayles²

1988

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

show abstract

“…The movement of anaphase B has been assumed to contain two different types; the involvement of polar MTs in one type (Cande and McDonald 1985) and the involvement of astral MTs in another type (Aist and Berns 1981). However, since anaphase A and B usually proceed simultaneously (Ris 1949, Oppenheim et al 1973, Izutsu et al 1977 analysis of such movements is difficult.…”

mentioning

confidence: 99%

The Arrangement of Microtubules in Mitotic Cells of Spirogyra.

Goto

Ueda

1995

CYTOLOGIA

View full text Add to dashboard Cite

“…Kinetochores have been reported to move towards poles in the process of polar sep aration in many algal and fungal cells, and prior to the polar separation in Barbulanympha. In animal cells, the chromosomal approach towards poles occurs while the poles are separating (Ris 1949, Boss 1954, Oppenheim et al 1973, Izutsu et al 1977. Thus, the movement of chromosomes in Spirogyra, which is strictly induced by the polar separation until late anaphase, may be a new style of movement.…”

Section: Discussionmentioning

confidence: 99%