The beneficial effect of natural antioxidants from olive oil with fig and date palm fruit extracts on biochemical and hematological parameters in rats during diethylnitrosamine-induced carcinogenesis

Abstract

Diethylnitrosamine (DEN) is a well-known carcinogen. The aim of our study was to determine the role of olive oil (7 g/kg) with fig (1 g/kg) (OF) and (or) date palm (1 g/kg) (D) fruit extracts during DEN treatment of male Wistar rats. The OF-DEN and (or) D-DEN groups were given oral antioxidants daily for two weeks before and during DEN treatment (21 weeks).The DEN-treated group showed dramatic results for all investigated parameters as compared with the control rats. All OF-DEN and D-DEN groups showed significant improvement in hepatic thiobarbituric acid reactive substances, reduced glutathione, and nitric oxide concentration in the liver tissue, in addition to improvement in serum vascular endothelial growth factor level, alpha-fetoprotein, lipid profile, lipid risk ratios, and the hematological parameters as compared with the DEN-treated group.In conclusion, the administration of OF and (or) D fruit extracts to DEN-treated rats resulted in a considerable improvement in the investigated biochemical and hematological parameters. In addition, the combined OFD treatments showed greater improvements revealing the synergistic effect of the combination.

The beneficial effect of natural antioxidants from olive oil with fig and date palm fruit extracts on biochemical and hematological parameters in rats treated with doxorubicin and γ-radiation

“…The administration of OFD to DOX-treated and (or) R-treated rats improves the hematological parameters (RBC, Hb, Hct, platelets, total, and differential WBCs), which might be attributed to increased antioxidant enzymes leading to diminished oxidative stress in bone marrow and the spleen. The results of our study are in agreement with the results of studies by Viola and Viola (2009) and Fathy et al (2018) who attributed the improved hematological parameters to oleuropein, a component of olive oil, which also exerts a favorable action on the platelets.…”

“…NO is a small, diffusible, highly reactive molecule that can generate oxidative stress (Millar 2004;Fathy et al 2018). The significant decrease in NO levels recorded in the liver tissue after DOX and (or) R treatment might be the result of its interaction with superoxide to form peroxynitrite (Pryor and Squadrito 1995).…”

“…This was demonstrated by significantly ameliorated oxidative stress markers (TBARS, GSH, and NO) in the liver tissue compared with the DOX-treated and (or) R-treated rats. This might be a result of the protective action of OFD active ingredients modifying membrane organization and their ability to scavenge oxidation-initiating agents (Bashandy et al 2014;Fathy et al 2018). The antioxidant effect of OFD is mainly due to phenolic compounds, which are able to donate a hydrogen atom to the free radicals, thus stopping the chain reaction propagation during the lipid peroxidation process (Sanchez-Mareno et al 1998).…”

“…The major benefit of the Mediterranean diet is its high level of antioxidants derived from fruits and vegetables, including olive oil, figs, and date palm fruits, which contribute antioxidant vitamins, minerals, flavonoids, and polyphenol content (Solomon et al 2006). In addition, mixed plant extracts showed a higher diversity of polyphenols resulting in greater stability and bioaccessibility of antioxidants compared with a single fruit extract (Bashandy et al 2014;Kamiloglu et al 2014;Fathy et al 2018).…”

Administration of pickled olive fruits treated with acid black nigrosine dye induced hepato-renal dysfunction in mice

“…The results showed that there was no mortality or toxic signs up to 5000 mg/kg and the % body weight change in POFE/B/KOH/ABND and the group of mice that were treated with ABND were 9.5% and -0.15%, respectively. The results of our study agree with the results of studies by Viola and Viola (2009) and Fathy et al (2018). The results showed that the activities of ALT and AST were increased in the group of mice that were treated with POFE/B/KOH/ABND, and ABND when compared to the normal control and NOFE control groups.…”