The Benefits From Update Laboratory Preanalytical Stage in PCR on the Way to Identify Occult Urological Infections in Male

Abstract

Aim of the study. Assess the diagnostic capabilities of detecting microorganisms in the wild ejaculate and sediment ejaculate. Materials and methods. 42 male volunteers were examined, aged 28 to 69, who were married and had children. Men applied for a preventive examination and to exclude asymptomatic male accessory gland infections. All patients had no active complaints characteristic of inflammatory diseases of the genitourinary tract. The study of the wild ejaculate and sediment ejaculate was performed by PCR real-time on DT-96 amplifiers (NPO DNA-Technology LLC, Russia) using the Androflor? test. Results. The value of the total bacterial mass in the sediment of the ejaculate is not statistically significantly higher compared to the wild ejaculate (p > 0.05). Candida spp. was statistically significantly more common in semen sediment (p < 0.05). In the sediment of the ejaculate were more often identified Enterobacteriaceae spp. / Enterococcus spp., Haemophilus spp., Staphylococcus spp., Streptococcus spp., Bacteroides spp. / Porphyromonas spp. / Prevotella spp. and Anaerococcus spp. (p > 0.05). In 11.9 % of observations, in the absence of bacteria in the wild ejaculate, microorganisms in low titers were identified in sediment ejaculate. Conclusion. According to the data obtained, the performance of the biological sample of the ejaculate sediment is higher compared to the sample of the wild ejaculate. The proposed modification of the preanalytical laboratory stage of the PCR technique, namely the diagnosis of ejaculate sediment using PCR real-time with the Androflor® test, makes it possible to more accurately determine pathogens, including those persisting in the form of biofilms in male accessory gland, even at low concentrations.