The Benefits of Olive Oil for Skin Health: Study on the Effect of Hydroxytyrosol, Tyrosol, and Oleocanthal on Human Fibroblasts

Abstract

Fibroblasts contribute to maintaining tissue integrity and homeostasis and are a key cell population in wound healing. This cell population can be stimulated by some bioactive compounds such as extra virgin olive oil (EVOO) polyphenols. The aim of this study was to determine the effects of hydroxytyrosol (htyr), tyrosol (tyr), and oleocanthal (ole) phenolic compounds present in EVOO on the proliferation, migration, cell cycle, and antigenic profile of cultured human fibroblasts. CCD-1064Sk human fibroblast cells were treated for 24 h with each polyphenol at doses ranging 10−5 to 10−9 M. Cell proliferation was evaluated using the MTT spectrophotometric technique, migration capacity by culture insert assay, and cell cycle and antigenic profile with flow cytometry. Cell proliferation was significantly increased by treatment with all compounds. The highest increases followed treatments with htyr or tyr at doses of 10−5 or 10−6 M and with ole at 10−6 and 10−7 M, and these compounds and doses were used for assays of antigenic profile, cell cycle, and migration. During the first few hours after treatment, increased fibronectin and α-actin expressions and greater cell migration were observed, with no cell cycle changes. In conclusion, these in vitro results suggest that phenolic compounds in EVOO might contribute to wound healing through action on fibroblasts related to tissue regeneration.

Effect of Punicalagin and Ellagic Acid on Human Fibroblasts In Vitro: A Preliminary Evaluation of Their Therapeutic Potential

“…In this in vitro study, the viability and migration of cultured human fibroblasts and their expression of fibronectin and α-actin were significantly increased by treatment with punicalagin or ellagic acid, phenolic compounds present in pomegranate, at doses of 10 −6 or 10 −7 M. No cell cycle alterations were produced by either treatment. Similar effects have also been reported after in vitro treatment of human fibroblasts with other bioactive substances of natural origin, such as the phenolic compounds in extra virgin olive oil, where the authors demonstrated an increase in the proliferation and migration of human fibroblasts in culture after 24 h of treatment with hydroxytyrosol, tyrosol, or oleocanthal at doses similar to those used in our study [33].…”

Natural Products and Health

“…Notably, no significant alterations were observed in cell cycle distribution or DNA integrity, suggesting the safety of these compounds. These findings underscore the potential of EVOO polyphenols in promoting tissue repair and regeneration, suggesting potential applications in wound healing processes such as cell adhesion, chemotaxis, and phagocytosis [21,22].…”

Identification and Evaluation of Olive Phenolics in the Context of Amine Oxidase Enzyme Inhibition and Depression: In Silico Modelling and In Vitro Validation

“…The BJ cells treated with OLP and OLC maintained their proliferation potential, similar to the untreated cells ( Figure S1 ). González-Acedo et al recently showed that the phenolic compounds HT and OLC significantly increased the growth capacity of human skin fibroblasts in comparison to untreated controls at certain doses [ 45 ].…”

“…The BJ cells treated with OLP and OLC maintained their proliferation potential, similar to the untreated cells (Figure S1). González-Acedo et al recently showed that the phenolic compounds HT and OLC significantly increased the growth capacity of human skin fibroblasts in comparison to untreated controls at certain doses [45]. As seen in Figure 5B, OLC (−8.4 kcal/mol) was predicted to have the strongest affinity for chain A of the MAO-B dimer, followed by OLP (−7.9 kcal/mol), HTA (−7.2 kcal/mol), and HT (−6.3 kcal/mol).…”