The benefits of Salvia sclarea L. ethanolic extracts on gastrointestinal and respiratory spasms

Randjelović, Milica; Branković, Suzana; Miladinović, Bojana; Milutinović, Milica; Zivanovic, Svetlana; Mihajilov‐Krstev, Tatjana; Kitić, Dušanka

doi:10.1016/j.sajb.2022.07.045

Cited by 7 publications

(22 citation statements)

References 55 publications

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“…In addition to the methanol extracts of S. sclarea , the inhibiting effects of excellent smooth muscle ileum contractions were reported for the S. sclarea ethanolic extracts and essential oil [ 18 , 48 ]. Rosmarinic acid, as the prevalent compound in the extracts, was proven to be an effective spasmolytic agent in in vitro conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The spasmolytic effects of the extracts were presented as an inhibition percentage of the contractile action of carbachol. Atropine was used as a positive control (0.41–100 μg/mL) [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…The relaxations of tracheal spasms by the extracts were expressed as a percentage of the contractile action inhibition carried out by the potassium ions. Verapamil was used as a positive control (0.41–100 μg/mL) [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

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An In Vitro and In Silico Characterization of Salvia sclarea L. Methanolic Extracts as Spasmolytic Agents

et al. 2023

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The use of medicinal plant species and their products is widespread in the field of gastrointestinal and respiratory diseases. This study aimed to evaluate the traditional use of Salvia sclarea L., clary sage, finding the possible mechanisms of its spasmolytic and bronchodilator actions in in vitro conditions supported by molecular docking analysis, along with the antimicrobial effects. Four dry extracts were prepared from the aerial parts of S. sclarea, using absolute or 80% (v/v) methanol by the method of a single-stage maceration or an ultrasound-assisted extraction. Characterization of the bioactive compounds by high-performance liquid chromatography indicated the presence of significant amounts of polyphenolics, with rosmarinic acid as the prevalent one. The spontaneous ileal contractions were best inhibited by the extract prepared with 80% methanol and maceration. The same extract was superior in the carbachol- and KCl-induced tracheal smooth muscle contractions, being the strongest bronchodilator agent. The most powerful relaxation of KCl-induced ileal contractions was achieved with the extract made of absolute methanol by maceration, while the 80% methanolic extract made with the ultrasound method generated the best spasmolytic effects in the acetylcholine-induced ileal contractions. Docking analysis suggested that apigenin-7-O-glucoside and luteolin-7-O-glucoside exhibited the highest binding affinity to voltage-gated calcium channels. Gram (+) bacteria were more susceptible to the effects of the extracts, particularly Staphylococcus aureus, in contrast to Gram (−) bacteria and Candida albicans. This is the first study to point out the influence of S. sclarea methanolic extracts on the gastrointestinal and respiratory spasm reduction, paving the way for their potential place in complementary medicine.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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An In Vitro and In Silico Characterization of Salvia sclarea L. Methanolic Extracts as Spasmolytic Agents

et al. 2023

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“…Since ancient times, EO obtained from Salvia species has been used due to its aromatic and medicinal properties, but nowadays it has a significant economic value in the perfume and cosmetics industries [ 13 ]. Traditionally, these species have been used as agents against inflammatory conditions (gingivitis, stomatitis, and aphthae), for the common cold and nervous fatigue, to increase lactation, and to prevent extreme sweating [ 8 , 13 , 14 ]. Reports made until now show that these species display a broad spectrum of biological activities such as antibacterial, anti-inflammatory, antifungal, antiviral, antiseptic, analgesic, antioxidant, anticancer, and antidiabetic [ 1 , 7 , 9 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…S. sclarea EO (SSEO) mainly consists of different types of terpene compounds such as monoterpene and sesquiterpene hydrocarbons, oxygenated monoterpenes and sesquiterpenes, aliphatic alcohols, and esters [ 13 ]. Among them, the most commonly found compounds are linalool and linalyl acetate as main compounds, followed by high amounts of α-terpineol, 1,8-cineole, sclareol, germacrene D, caryophyllene, and caryophyllene oxide [ 8 , 10 , 14 , 15 , 16 ]. These volatile compounds are found to be responsible for the reported beneficial effects of SSEO.…”

Section: Introductionmentioning

confidence: 99%

Salvia sclarea Essential Oil Chemical Composition and Biological Activities

Kačániová

Vuković

Čmiková

et al. 2023

IJMS

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Salvia sclarea essential oil (SSEO) has a long tradition in the food, cosmetic, and perfume industries. The present study aimed to analyze the chemical composition of SSEO, its antioxidant activity, antimicrobial activity in vitro and in situ, antibiofilm, and insecticidal activity. Besides that, in this study, we have evaluated the antimicrobial activity of SSEO constituent (E)-caryophyllene and standard antibiotic meropenem. Identification of volatile constituents was performed by using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) techniques. Results obtained indicated that the main constituents of SSEO were linalool acetate (49.1%) and linalool (20.6%), followed by (E)-caryophyllene (5.1%), p-cimene (4.9%), a-terpineol (4.9%), and geranyl acetate (4.4%). Antioxidant activity was determined as low by the means of neutralization of the DDPH radical and ABTS radical cation. The SSEO was able to neutralize the DPPH radical to an extent of 11.76 ± 1.34%, while its ability to decolorize the ABTS radical cation was determined at 29.70 ± 1.45%. Preliminary results of antimicrobial activity were obtained with the disc diffusion method, while further results were obtained by broth microdilution and the vapor phase method. Overall, the results of antimicrobial testing of SSEO, (E)-caryophyllene, and meropenem, were moderate. However, the lowest MIC values, determined in the range of 0.22–0.75 µg/mL for MIC50 and 0.39–0.89 µg/mL for MIC90, were observed for (E)-caryophyllene. The antimicrobial activity of the vapor phase of SSEO (towards microorganisms growing on potato) was significantly stronger than that of the contact application. Biofilm analysis using the MALDI TOF MS Biotyper showed changes in the protein profile of Pseudomonas fluorescens that showed the efficiency of SSEO in inhibiting biofilm formation on stainless-steel and plastic surfaces. The insecticidal potential of SSEO against Oxycarenus lavatera was also demonstrated, and results show that the highest concentration was the most effective, showing insecticidal activity of 66.66%. The results obtained in this study indicate the potential application of SSEO as a biofilm control agent, in the shelf-life extension and storage of potatoes, and as an insecticidal agent.

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Salvia sclarea L. (Clary Sage) flower extract-mediated green synthesis of silver nanoparticles and their characterization, antibacterial, antifungal, and scolicidal activities against Echinococcus granulosus

Zarei,

Razmjoue,

Moazeni

et al. 2024

The Microbe

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The benefits of Salvia sclarea L. ethanolic extracts on gastrointestinal and respiratory spasms

Cited by 7 publications

References 55 publications

An In Vitro and In Silico Characterization of Salvia sclarea L. Methanolic Extracts as Spasmolytic Agents

An In Vitro and In Silico Characterization of Salvia sclarea L. Methanolic Extracts as Spasmolytic Agents

Salvia sclarea Essential Oil Chemical Composition and Biological Activities

Salvia sclarea L. (Clary Sage) flower extract-mediated green synthesis of silver nanoparticles and their characterization, antibacterial, antifungal, and scolicidal activities against Echinococcus granulosus

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