The between- and within-grape-cultivars genetic variation

Stavrakakis, Manolis; Loukas, M.

doi:10.1016/0304-4238(83)90080-8

Cited by 23 publications

(14 citation statements)

References 12 publications

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“…Other methods of genetic variation analysis have been used with varying degrees of success. Genotyping difficulties are directly related to the genetic relationships between samples and the number of selected markers [21][22][23]. Furthermore, molecular markers have also been extensively used to discriminate among Vitis vinifera clones of the same cultivars [24][25][26][27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Study of Intra-Varietal Genetic Variability in Grapevine Cultivars by PCR-Derived Molecular Markers and Correlations with the Geographic Origins

et al. 2011

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The genetic grapevine intravarietal variability will be analyzed by PCR-derived marker systems. In particular, the object of the investigation will be the clonal variations of Malvasia nera di Brindisi/Lecce, Negroamaro and Primitivo, also known as Zinfandel, which are three grapevine varieties cultivated in Apulia region (Italy). In order to assess varietal identity of the samples, 132 DNA tests were performed by amplifying 16 SSR loci. The study of the intravarietal variability was performed using AFLPs, SAMPLs, ISSRs, and M-AFLPs. The application of the above-mentioned techniques allowed both to discriminate all genotypes of the three cultivars and to distinguish the accessions of each cultivar sampled from different geographic cultivation areas. Furthermore, the study of biotypes cultivated in different geographical environments of Salento (i.e., Apulia region) allowed important correlations between molecular marker variability and phenotypic traits. These results are suggesting both to focus our attention on the effects of the environment on the genotype and to consider, as a practical consequence, the importance of preserving autochthon grapevine biotypes found in different areas to truly preserve the richness of the germplasm. Thus, more accurate DNA studies give new information that can be extremely useful to the vine nurseries for the correct choice (i.e., supported by more accurate intravarietal variability analysis) of the grape multiplication materials.

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Section: Introductionmentioning

confidence: 99%

Study of Intra-Varietal Genetic Variability in Grapevine Cultivars by PCR-Derived Molecular Markers and Correlations with the Geographic Origins

et al. 2011

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“…Similar studies have been performed when the inheritance of electrophoretic bands is not known ; for example, Fedak & Rajhathy (1972) in barley, Jensen et al . (1979) Stavrakakis & Loukas (1983) in Vitis etc . A word of caution seems to be necessary .…”

Section: Methodsmentioning

confidence: 99%

Geographic dispersion and varietal diversity in Vicia faba L.

Serradilla¹,

Mora²,

Moreno

1993

Genet Resour Crop Evol

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Twenty-six faba bean (Vicia faba L .) populations, from five geographic areas and five varietal types have been assayed electrophoretically for variation in seven isozyme systems . Isozyme frequencies in populations, botanical varieties and geographical areas were submitted to the principal component analysis (PCA) . Varieties grouped together showed no differences among them except for isolated populations . Populations from Ethiopia and China lay far apart in PCA from those of other origins . It seems that no genetic divergence at the isozyme level took place during the varietal formation and dispersion process, except when geographical isolation of the population occurred . The results support the hypothesis of an isolation of the crop in Ethiopia and in China .

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“…It has been estimated that there are more than 8,000 grape cultivars, under 24 000 different names (Viala & Vermorel, 1909). In Greece more than 300 grapevine cultivars (Vitis vinifera L.) are grown and classic ampelographic (Krimbas, 1943;Davidis, 1967;Vlachos, 1986;Stavrakakis, 2010), biochemical (Stavrakakis & Loukas, 1983) and molecular methods (Stavrakakis at al., 1997;Stavrakakis & Biniari, 1998;Stavrakaki, 2008) have been used for their discrimination and classification. Among the various polymerase chain reaction (PCR)-based DNA marker techniques available, the Amplified Fragment Length Polymorphism (AFLP) is often used, because it is ideal when the goal is the definition of identity among different clones of the same variety or among genetically close related cultivars, with positive results in differentiating grapevine cultivars and clones (Vignani et al, 2002;As far as its origin is concerned, the ampelographic characters classify Korinthiaki staphis as well as Korinthiaki lefki to proles pontica, to which most Greek grapevine varieties belong and to sub-proles balcanica (Negrul, 1938(Negrul, , 1946Levadoux, 1956).…”

Section: Introductionmentioning

confidence: 99%

Genotyping and Phenotyping of the Potential Clones, Biotypes and Variants of Grapevine Cultivar Korinthiaki Staphis (Vitis vinifera L.)

Stavrakaki¹,

Biniari²

2016

JAS

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This study presents the results regarding the identification and discrimination of twenty seven possible clones of grapevine cultivar Korinthiaki staphis (Vitis vinifera L.), three biotypes of Korinthiaki lefki and the related cultivar Staphidampelo using the ampelographic description and the molecular method AFLP. The results from the statistical analysis showed that all the biotypes of cultivar Korinthiaki staphis show small distance and are grouped in the same cluster, depending on their origin, while Staphidampelo and Korinthiaki lefki are neither variants nor biotypes of the cultivar Korinthiaki staphis but different cultivars since they are very distant compared to the other biotypes and moreover, Korinthiaki lefki is in a separate cluster of the dendrogram. The ampelographic description in combination with the molecular method AFLP are effective for the study of the between and within genetic diversity of grapevine cultivars as well as for their identification and discrimination. The results of this study can constitute the base for the implementation of the clonal selection for grapevine cultivar Korinthiaki staphis and the seclusion of the desired clones.

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The between- and within-grape-cultivars genetic variation

Cited by 23 publications

References 12 publications

Study of Intra-Varietal Genetic Variability in Grapevine Cultivars by PCR-Derived Molecular Markers and Correlations with the Geographic Origins

Study of Intra-Varietal Genetic Variability in Grapevine Cultivars by PCR-Derived Molecular Markers and Correlations with the Geographic Origins

Geographic dispersion and varietal diversity in Vicia faba L.

Genotyping and Phenotyping of the Potential Clones, Biotypes and Variants of Grapevine Cultivar Korinthiaki Staphis (Vitis vinifera L.)

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