The bHLH transcription factor AhbHLH121 improves salt tolerance in peanut

Zhao, Xiaobo; Wang, Qi; Yan, Caixia; Sun, Quanxi; Wang, Juan; Li, Chunjuan; Yuan, Cuiling; Mou, Yifei; Shan, Shihua

doi:10.1016/j.ijbiomac.2023.128492

Cited by 17 publications

(6 citation statements)

References 54 publications

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“…In Arabidopsis , the WRKY53 transcription factor can regulate leaf senescence progression by inhibiting the expression level of the SENRK1 gene [ 51 ]. AhbHLH121 improves salt tolerance in the peanut by activating the expression of AhPOD , AhCAT , and AhSOD [ 52 ]. Significantly, it was observed that the AhSPL genes’ promoter regions contained multiple anticipated binding sites for transcription factors, such as ERF, NAC, MYB, Dof, and MICK_MADS ( Figure 7 ).…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Analysis of the Peanut SQUAMOSA Promoter Binding Protein-like Gene Family and How AhSPL5 Enhances Salt Tolerance in Transgenic Arabidopsis

Sun,

Zhang,

et al. 2024

Plants

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SPL (SQUAMOSA promoter binding protein-like), as one family of plant transcription factors, plays an important function in plant growth and development and in response to environmental stresses. Despite SPL gene families having been identified in various plant species, the understanding of this gene family in peanuts remains insufficient. In this study, thirty-eight genes (AhSPL1-AhSPL38) were identified and classified into seven groups based on a phylogenetic analysis. In addition, a thorough analysis indicated that the AhSPL genes experienced segmental duplications. The analysis of the gene structure and protein motif patterns revealed similarities in the structure of exons and introns, as well as the organization of the motifs within the same group, thereby providing additional support to the conclusions drawn from the phylogenetic analysis. The analysis of the regulatory elements and RNA-seq data suggested that the AhSPL genes might be widely involved in peanut growth and development, as well as in response to environmental stresses. Furthermore, the expression of some AhSPL genes, including AhSPL5, AhSPL16, AhSPL25, and AhSPL36, were induced by drought and salt stresses. Notably, the expression of the AhSPL genes might potentially be regulated by regulatory factors with distinct functionalities, such as transcription factors ERF, WRKY, MYB, and Dof, and microRNAs, like ahy-miR156. Notably, the overexpression of AhSPL5 can enhance salt tolerance in transgenic Arabidopsis by enhancing its ROS-scavenging capability and positively regulating the expression of stress-responsive genes. These results provide insight into the evolutionary origin of plant SPL genes and how they enhance plant tolerance to salt stress.

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Section: Discussionmentioning

confidence: 99%

A Comprehensive Analysis of the Peanut SQUAMOSA Promoter Binding Protein-like Gene Family and How AhSPL5 Enhances Salt Tolerance in Transgenic Arabidopsis

Sun,

Zhang,

et al. 2024

Plants

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“…87 In Arachis hypogaea, AhbHLH121 binds to the G/E-box regions of the POD, CAT, and SOD promoters and activates their expression, thereby enhancing salt stress resistance. 27,28 Most recently, Yan et al reported that ZmbHLH32 binds to the E-box in the of promoter of ZmIAA9 and activates its expression that positively regulates salt tolerance in maize. 230 Collectively, these findings highlight the pivotal role of bHLH as a core regulator that confers salt tolerance by directly interacting with both proteins and DNA.…”

Section: Salt Stressmentioning

confidence: 99%

“…In Zoysia japonica , ZjICE1/2 not only serve as positive regulators in cold signaling but also play a crucial role in drought and salt signaling pathways. , In soybean, GmbHLH3 could activate the expression of TONOPLAST-LOCALIZED CL – / H + ANTIPORTER ( GmCLC1 ) and thus maintain the anion homeostasis under salt stress . In Arachis hypogaea , AhbHLH121 binds to the G/E-box regions of the POD , CAT , and SOD promoters and activates their expression, thereby enhancing salt stress resistance. , Most recently, Yan et al reported that ZmbHLH32 binds to the E-box in the of promoter of ZmIAA9 and activates its expression that positively regulates salt tolerance in maize . Collectively, these findings highlight the pivotal role of bHLH as a core regulator that confers salt tolerance by directly interacting with both proteins and DNA.…”

Section: The Function Of Bhlh Transcription Factors In Abiotic Stress...mentioning

confidence: 99%

Functions of Basic Helix–Loop–Helix (bHLH) Proteins in the Regulation of Plant Responses to Cold, Drought, Salt, and Iron Deficiency: A Comprehensive Review

Lei,

Jiang,

Zhao

et al. 2024

J. Agric. Food Chem.

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Abiotic stresses including cold, drought, salt, and iron deficiency severely impair plant development, crop productivity, and geographic distribution. Several bodies of research have shed light on the pleiotropic functions of BASIC HELIX− LOOP−HELIX (bHLH) proteins in plant responses to these abiotic stresses. In this review, we mention the regulatory roles of bHLH TFs in response to stresses such as cold, drought, salt resistance, and iron deficiency, as well as in enhancing grain yield in plants, especially crops. The bHLH proteins bind to E/G-box motifs in the target promoter and interact with various other factors to form a complex regulatory network. Through this network, they cooperatively activate or repress the transcription of downstream genes, thereby regulating various stress responses. Finally, we present some perspectives for future research focusing on the molecular mechanisms that integrate and coordinate these abiotic stresses. Understanding these molecular mechanisms is crucial for the development of stress-tolerant crops.

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“…We used virus-induced gene silencing to explore the potential function of AhBBX6 in salt and drought tolerance. The octahydro lycopene desaturase (PDS) gene in peanuts (Arahy.M5MKEZ) was chosen as an indicator gene for the success of the VIGS system [31,32]. Based on qRT-PCR, the relative expression of PDS-encoding genes was significantly decreased in AhPDS-silenced plants compared to the control plants (pTRV2/0) (Figure S2).…”

Section: Silencing Of Ahbbx6 Reduces Tolerance To Salt and Drought St...mentioning

confidence: 99%

Genome-Wide Identification of Peanut B-Boxs and Functional Characterization of AhBBX6 in Salt and Drought Stresses

Tang,

Yuan,

Shi

et al. 2024

Plants

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The B-box (BBX) gene family includes zinc finger protein transcription factors that regulate a multitude of physiological and developmental processes in plants. While BBX gene families have been previously determined in various plants, the members and roles of peanut BBXs are largely unknown. In this research, on the basis of the genome-wide identification of BBXs in three peanut species (Arachis hypogaea, A. duranensis, and A. ipaensis), we investigated the expression profile of the BBXs in various tissues and in response to salt and drought stresses and selected AhBBX6 for functional characterization. We identified a total of 77 BBXs in peanuts, which could be grouped into five subfamilies, with the genes from the same branch of the same subgroup having comparable exon–intron structures. In addition, a significant number of cis-regulatory elements involved in the regulation of responses to light and hormones and abiotic stresses were found in the promoter region of peanut BBXs. Based on the analysis of transcriptome data and qRT-PCR, we identified AhBBX6, AhBBX11, AhBBX13, and AhBBX38 as potential genes associated with tolerance to salt and drought. Silencing AhBBX6 using virus-induced gene silencing compromised the tolerance of peanut plants to salt and drought stresses. The results of this study provide knowledge on peanut BBXs and establish a foundation for future research into their functional roles in peanut development and stress response.

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The bHLH transcription factor AhbHLH121 improves salt tolerance in peanut

Cited by 17 publications

References 54 publications

A Comprehensive Analysis of the Peanut SQUAMOSA Promoter Binding Protein-like Gene Family and How AhSPL5 Enhances Salt Tolerance in Transgenic Arabidopsis

A Comprehensive Analysis of the Peanut SQUAMOSA Promoter Binding Protein-like Gene Family and How AhSPL5 Enhances Salt Tolerance in Transgenic Arabidopsis

Functions of Basic Helix–Loop–Helix (bHLH) Proteins in the Regulation of Plant Responses to Cold, Drought, Salt, and Iron Deficiency: A Comprehensive Review

Genome-Wide Identification of Peanut B-Boxs and Functional Characterization of AhBBX6 in Salt and Drought Stresses

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