The bHLH Transcription Factor MYC3 Interacts with the Jasmonate ZIM-Domain Proteins to Mediate Jasmonate Response in Arabidopsis

Cheng, Zhiwei; Sun, Li; Qi, Tiancong; Zhang, Bosen; Wen, Peng; Liu, Yule; Xie, Daoxin

doi:10.1093/mp/ssq073

Cited by 249 publications

(216 citation statements)

References 51 publications

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“…Upon perception of JA signal (Fonseca et al, 2009;Yan et al, 2009;Sheard et al, 2010), the F-box protein CORO-NATINE INSENSITIVE1 (COI1) (Xie et al, 1998;Yan et al, 2009) recruits the JASMONATE ZIM-DOMAIN (JAZ) proteins (Chini et al, 2007;Thines et al, 2007;Yan et al, 2007) for degradation, which leads to the release of various downstream factors, including MYC2/JASMONATE INSENSITIVE1 (JIN1), MYC3, and MYC4 (Cheng et al, 2011;Fernández-Calvo et al, 2011;Niu et al, 2011), as well as WD-repeat/bHLH/MYB complex , MYB21, MYB24, and MYB57 (Mandaokar et al, 2006;Song et al, 2011) and the IIId bHLH factors (Nakata et al, 2013;Song et al, 2013b), which regulate diverse JA-mediated functions. These functions include root growth (Dathe et al, 1981;Chen et al, 2011), apical hook formation (Turner et al, 2002), flowering (Robson et al, 2010), stamen development (McConn and Browse, 1996;Song et al, 2011Song et al, , 2013a, leaf senescence (Ueda and Kato, 1980;Shan et al, 2011), secondary metabolism (De Geyter et al, 2012;Schweizer et al, 2013), drought responses (Seo et al, 2011), wounding responses (Mason and Mullet, 1990;Acosta et al, 2013;Mousavi et al, 2013), and defense against pathogen infection Vijayan et al, 1998;Melotto et al, 2006;Rowe et al, 2010;Yang et al, 2012;Zheng et al, 2012) and insect attack (McConn et al, 1997;…”

Section: Introductionmentioning

confidence: 99%

Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

et al. 2014

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Plants have evolved sophisticated mechanisms for integration of endogenous and exogenous signals to adapt to the changing environment. Both the phytohormones jasmonate (JA) and ethylene (ET) regulate plant growth, development, and defense. In addition to synergistic regulation of root hair development and resistance to necrotrophic fungi, JA and ET act antagonistically to regulate gene expression, apical hook curvature, and plant defense against insect attack. However, the molecular mechanism for such antagonism between JA and ET signaling remains unclear. Here, we demonstrate that interaction between the JA-activated transcription factor MYC2 and the ET-stabilized transcription factor ETHYLENE-INSENSITIVE3 (EIN3) modulates JA and ET signaling antagonism in Arabidopsis thaliana. MYC2 interacts with EIN3 to attenuate the transcriptional activity of EIN3 and repress ET-enhanced apical hook curvature. Conversely, EIN3 interacts with and represses MYC2 to inhibit JA-induced expression of wound-responsive genes and herbivory-inducible genes and to attenuate JA-regulated plant defense against generalist herbivores. Coordinated regulation of plant responses in both antagonistic and synergistic manners would help plants adapt to fluctuating environments.

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Section: Introductionmentioning

confidence: 99%

Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

et al. 2014

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“…MYC2 directly activates the expression of JAZs and several JA biosynthetic genes (Chini et al, 2007;Thines et al, 2007;Hou et al, 2010). The MYC3 and MYC4 transcription factors are closely related to MYC2 and act additively with MYC2 to regulate different subsets of JA response genes (Cheng et al, 2011;Fernández-Calvo et al, 2011;Niu et al, 2011). However, myc2 myc3 myc4 triple mutants are partially responsive to JA and, unlike the male sterile coi1 and JAZ gain-of-function mutants, are male fertile (Feys et al, 1994;Thines et al, 2007;Chung and Howe, 2009;Fernández-Calvo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling inArabidopsis

et al. 2013

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Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loophelix-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth.

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“…The perception of JA-Ile induces the interaction between COI1 and JAZs, which brings JAZs for degradation and relieves their repression on MYC2 (23,26). Two MYC2-like bHLH proteins (MYC3 and MYC4) were also able to interact with JAZs and act addictively with MYC2 in mediating a subset of JA-regulated responses, including inhibition of root elongation, wound response, and metabolism (29)(30)(31). Besides MYC2/MYC3/MYC4, two MYB transcription factors (MYB21 and MYB24) were recently identified as JAZ targets in regulating plant stamen development (32).…”

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confidence: 99%

Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis

Zhu

An²,

Feng³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

635

528

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Jasmonate (JA) and ethylene (ET) are two major plant hormones that synergistically regulate plant development and tolerance to necrotrophic fungi. Both JA and ET induce the expression of several pathogenesis-related genes, while blocking either signaling pathway abolishes the induction of these genes by JA and ET alone or in combination. However, the molecular basis of JA/ET coaction and signaling interdependency is largely unknown. Here, we report that two Arabidopsis ET-stabilized transcription factors (EIN3 and EIL1) integrate ET and JA signaling in the regulation of gene expression, root development, and necrotrophic pathogen defense. Further studies reveal that JA enhances the transcriptional activity of EIN3/EIL1 by removal of JA-Zim domain (JAZ) proteins, which physically interact with and repress EIN3/EIL1. In addition, we find that JAZ proteins recruit an RPD3-type histone deacetylase (HDA6) as a corepressor that modulates histone acetylation, represses EIN3/EIL1-dependent transcription, and inhibits JA signaling. Our studies identify EIN3/EIL1 as a key integration node whose activation requires both JA and ET signaling, and illustrate transcriptional derepression as a common mechanism to integrate diverse signaling pathways in the regulation of plant development and defense.root hair | Botrytis cinerea P lants are sessile organisms and face different environmental changes during their lifespan. To survive various abiotic and biotic stresses, plants synthesize a number of small molecules functioning as phytohormones to elaborately regulate their growth, development, and defense. Two types of phytohormonesethylene (ET) and jasmonate (JA)-are crucial for plant development and defense against necrotrophic fungi infections (1-3). Complicated modes of interaction between ET and JA have been documented in different processes. For example, ET strongly suppresses JA-induced wounding-responsive gene expression, but JA suppresses ET-induced apical hook formation (4, 5), indicative of their antagonisms. Upon necrotrophic fungi infections, plants can quickly produce ET and JA and induce the expression of downstream defense genes (like ERF1, ORA59, and PDF1.2) that help plants tolerate or fight against the fungal pathogens (1). Plants treated with exogenous JA or ET express high levels of defense genes (6, 7), and simultaneous treatment with JA and ET results in the highest expression (8). Nevertheless, in the ET or JA insensitive mutant (ein2 or coi1, respectively), JA and ET alone or in combination fail to induce the expression of those defense genes (8, 9), indicating that the two hormone-signaling pathways are required concomitantly for the activation of plant-defense response. These results suggest that JA and ET act synergistically and mutually dependently in regulating necrotrophic pathogen responses. However, the molecular details underlying such hormone synergy and signaling interdependency are currently unknown.ET is a gaseous hormone, which is perceived by its receptors and represses a Raf-like kinase CON...

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The bHLH Transcription Factor MYC3 Interacts with the Jasmonate ZIM-Domain Proteins to Mediate Jasmonate Response in Arabidopsis

Cited by 249 publications

References 51 publications

Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling inArabidopsis

Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis

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