The Bifidogenic Growth Stimulator Inhibits the Growth and Respiration of <i>Helicobacter pylori</i>

Nagata, Kumiko; Inatsu, Sakiko; Tanaka, Mamoru; Sato, Harumi; Kouya, Tomoaki; Taniguchi, Masayuki; Fukuda, Yoshihiro

doi:10.1111/j.1523-5378.2010.00789.x

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…The results differed depending on individual foods or bacteria. For example, Wasabia japonica leaf, 7 rice extract, 8 a strain of Bifidobacterium bifidum, 9 and garlic 10 suppressed mucosal inflammation in the animal stomach, while broccoli sprouts, 11 Lacidofil, 12 rice-fluid, 13 1,4-di-hydroxy-2-naphthoic acid (a kind of prebiotic stimulating the growth of Bifidobacterium), 14 and Lactobacillus 15,16 suppressed both growth of HP and gastric mucosal inflammation. Such kinds of anti-HP foods and probiotics were, therefore, promising, but while these results brought them closer to clinical application, but considerable obstacles still remained.…”

Section: Masashi Matsushima and Atsushi Takagimentioning

confidence: 99%

Visceral obesity modulates the impact of apolipoprotein C3 gene variants on liver fat content

et al. 2011

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Objective: It has not been solved whether subjects carrying the minor alleles of the À455T4C or À482C4T single nucleotide polymorphisms (SNPs) in the apolipoprotein-C3-gene (APOC3) have an increased risk for developing fatty liver and insulin resistance. We investigated the relationships of the SNPs with hepatic APOC3 expression and hypothesized that visceral obesity may modulate the effects of these SNPs on liver fat and insulin sensitivity (IS). Methods: APOC3 mRNA expression and triglyceride content were determined in liver biopsies from 50 subjects. In a separate group (N ¼ 330) liver fat was measured by 1 H-magnetic resonance spectroscopy. IS was estimated during an oral glucose tolerance test (OGTT) and the euglycemic, hyperinsulinemic clamp (N ¼ 222). Results: APOC3 mRNA correlated positively with triglyceride content in liver biopsies (r ¼ 0.29, P ¼ 0.036). Carriers of the minor alleles (À455C and À482T) tended to have higher hepatic APOC3 mRNA expression (1.80 (0.45-3.56) vs 0.77 (0.40-1.64), P ¼ 0.09), but not higher triglyceride content (P ¼ 0.76). In 330 subjects the genotype did not correlate with liver fat (P ¼ 0.97) or IS (OGTT: P ¼ 0.41; clamp: P ¼ 0.99). However, a significant interaction of the genotype with waist circumference in determining liver fat was detected (P ¼ 0.02) in which minor allele carriers had higher liver fat only in the lowest tertile of waist circumference (P ¼ 0.01). In agreement, during a 9-month lifestyle intervention the minor allele carriers of the SNP À482C4T in the lowest tertile also had less decrease in liver fat (P ¼ 0.04). Conclusions: APOC3 mRNA expression is increased in fatty liver and is regulated by SNPs in APOC3. The impact of the APOC3 SNPs on fatty liver is small and depends on visceral obesity.

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Section: Masashi Matsushima and Atsushi Takagimentioning

confidence: 99%

Visceral obesity modulates the impact of apolipoprotein C3 gene variants on liver fat content

et al. 2011

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“…It has been normally treated by triple therapy with amoxicillin, clarithromycin, and a proton-pump inhibitor but clarithromycin-resistant strains have been frequently appeared. DHNA could inhibit clinical H. pylori isolates resistant to the antibiotics by disrupting cellular respiration and ATP generation ( Nagata et al , 2010 ). Therefore DHNA could also be a good pharmaceutical candidate for treatment of IBD, antibioticresistant pathogenic bacteria, etc.…”

Section: Resultsmentioning

confidence: 99%

Construction of a Recombinant Leuconostoc mesenteroides CJNU 0147 Producing 1,4-Dihydroxy-2-Naphthoic Acid, a Bifidogenic Growth Factor

Eom¹,

Moon²

2015

Korean Journal for Food Science of Animal Resources

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1,4-Dihydroxy-2-naphthoic acid (DHNA), a precursor of menaquinone (vitamin K2), has an effect on growth stimulation of bifidobacteria and prevention of osteoporosis, making it a promising functional food material. Therefore, we tried to clone the menB gene encoding DHNA synthase from Leuconostoc mesenteroides CJNU 0147. Based on the genome sequence of Leu. mesenteroides ATCC 8293 (GenBank accession no., CP000414), a primer set (Leu_menBfull_F and Leu_menBfull_R) was designed for the PCR amplification of menB gene of CJNU 0147. A DNA fragment (1,190 bp), including the menB gene, was amplified, cloned into pGEM-T Easy vector, and sequenced. The deduced amino acid sequence of MenB (DHNA synthase) protein of CJNU 0147 had a 98% similarity to the corresponding protein of ATCC 8293. The menB gene was subcloned into pCW4, a lactic acid bacteria - E. coli shuttle vector, and transferred to CJNU 0147. The transcription of menB gene of CJNU 0147 (pCW4::menB) was increased, when compared with those of CJNU 0147 (pCW4) and CJNU 0147 (−). The DHNA was produced from it at a detectable level, indicating that the cloned menB gene of CJNU 0147 encoded a DHNA synthase which is responsible for the production of DHNA, resulting in an increase of bifidogenic growth stimulation activity.

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“…DHNA has a specific and strong growth stimulating activity on Bifidobacterium species at extremely low concentrations (Isawa et al, 2002). On the other hand, DHNA inhibits growth and respiration of the pathogenic species Helicobacter pylori (Nagata et al, 2010). Recent work suggested that DHNA may be a key bacterial metabolite playing roles in host-microbe interaction, intestinal function, and improving the immune system (Matsubara et al, 2010;Fukumoto et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

A partial metabolic pathway enables group b streptococcus to overcome quinone deficiency in a host bacterial community

Franza

Delavenne

Derré-Bobillot

et al. 2016

Molecular Microbiology

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Aerobic respiration metabolism in Group B Streptococcus (GBS) is activated by exogenous heme and menaquinone. This capacity enhances resistance of GBS to acid and oxidative stress and improves its survival. In this work, we discovered that GBS is able to respire in the presence of heme and 1,4-dihydroxy-2-naphthoic acid (DHNA). DHNA is a biosynthetic precursor of demethylmenaquinone (DMK) in many bacterial species. A GBS gene (gbs1789) encodes a homolog of the MenA 1,4-dihydroxy-2-naphthoate prenyltransferase enzyme, involved in the synthesis of demethylmenaquinone. In this study, we showed that gbs1789 is involved in the biosynthesis of long-chain demethylmenaquinones (DMK-10). The Δgbs1789 mutant cannot respire in the presence of heme and DHNA, indicating that endogenously synthesized DMKs are cofactors of the GBS respiratory chain. We also found that isoprenoid side chains from GBS DMKs are produced by the protein encoded by the gbs1783 gene, since this gene can complement an Escherichia coli ispB mutant defective for isoprenoids chain synthesis. In the gut or vaginal microbiote, where interspecies metabolite exchanges occur, this partial DMK biosynthetic pathway can be important for GBS respiration and survival in different niches.

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The Bifidogenic Growth Stimulator Inhibits the Growth and Respiration of Helicobacter pylori

Cited by 14 publications

References 37 publications

Visceral obesity modulates the impact of apolipoprotein C3 gene variants on liver fat content

Visceral obesity modulates the impact of apolipoprotein C3 gene variants on liver fat content

Construction of a Recombinant Leuconostoc mesenteroides CJNU 0147 Producing 1,4-Dihydroxy-2-Naphthoic Acid, a Bifidogenic Growth Factor

A partial metabolic pathway enables group b streptococcus to overcome quinone deficiency in a host bacterial community

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