“The big sleep: Elucidating the sequence of events in the first hours of death to determine the postmortem interval”

Martínez, Paula Núñez; Menéndez, Sofía T.; Villaronga, María de los Ángeles; Ubelaker, Douglas H.; García-Pedrero, Juana M.; Zapico, Sara C.

doi:10.1016/j.scijus.2019.03.001

Cited by 7 publications

(4 citation statements)

References 80 publications

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“…It has been reported that in senescent cells there is a downregulation of SIRT1 protein due to autophagy throughout LC3 ( Xu et al, 2020 ). In fact, at the postmortem interval, an over-expression of some genes associated with autophagy as LC3, ATG7 or ATG12 has been found ( Martínez et al, 2019 ). Also, SIRT1 regulates pro-inflamatory response through modulation of NF-κB signaling ( Kim, Silwal & Jo, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

A miRNome analysis at the early postmortem interval

Guardado-Estrada

Cárdenas-Monroy

Martínez-Rivera

et al. 2023

PeerJ

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The postmortem interval (PMI) is the time elapsing since the death of an individual until the body is examined. Different molecules have been analyzed to better estimate the PMI with variable results. The miRNAs draw attention in the forensic field to estimate the PMI as they can better support degradation. In the present work, we analyzed the miRNome at early PMI in rats’ skeletal muscle using the Affymetrix GeneChip™ miRNA 4.0 microarrays. We found 156 dysregulated miRNAs in rats’ skeletal muscle at 24 h of PMI, out of which 84 were downregulated, and 72 upregulated. The miRNA most significantly downregulated was miR-139-5p (FC = −160, p = 9.97 × 10−11), while the most upregulated was rno-miR-92b-5p (FC = 241.18, p = 2.39 × 10−6). Regarding the targets of these dysregulated miRNAs, the rno-miR-125b-5p and rno-miR-138-5p were the miRNAs with more mRNA targets. The mRNA targets that we found in the present study participate in several biological processes such as interleukin secretion regulation, translation regulation, cell growth, or low oxygen response. In addition, we found a downregulation of SIRT1 mRNA and an upregulation of TGFBR2 mRNA at 24 h of PMI. These results suggest there is an active participation of miRNAs at early PMI which could be further explored to identify potential biomarkers for PMI estimation.

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Section: Discussionmentioning

confidence: 99%

A miRNome analysis at the early postmortem interval

Guardado-Estrada

Cárdenas-Monroy

Martínez-Rivera

et al. 2023

PeerJ

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“…A recent publication from Martinez et al [53] analyzed the expression of autophagy genes during the early PMI (0-8 h) in rat gastrocnemius muscle. Autophagy is an essential process that maintains cellular homeostasis via the degradation and recycling of long-lived proteins, intracellular aggregates, and damaged organelles [54].…”

Section: Rnamentioning

confidence: 99%

Postmortem Interval Estimation: New Approaches by the Analysis of Human Tissues and Microbial Communities’ Changes

Zapico

Adserias‐Garriga

2022

Forensic Sciences

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There are several methodologies available to estimate time since death based on different changes that a corpse undergoes after death. However, these methods are imprecise due to the decomposition process being affected by several factors, principally temperature and humidity. Current trends for the determination of the Postmortem Interval (PMI) attempt to estimate the PMI in a quantifiable manner, based on chemical changes on and in the body, summarized in the field of “thanatochemistry”. Although these methodologies have improved PMI estimates, additional research has been developed to increase the accuracy and precision of this determination. As a result, the fields of “thanatobiology” and “thanatomicrobiome” have emerged. Thanatobiology is based on the estimation of the PMI from DNA/RNA degradation, signaling pathways of cell death, and protein analysis. Thanatomicrobiome refers to changes in the bacterial communities as a consequence of the decomposition process. Although both approaches seem to improve PMI estimates, applications of thanatobiology methodologies are more appropriate in the first phases of decomposition, while thanatomicrobiome analyses are applicable in advanced stages. Further research is needed in these new fields in order to establish their applicability in forensic cases. This is a review of the current state-of-the-art methodology in these two subfields.

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“…In rats, the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) increases within 4 h after death, then after 6 h decreases slightly to continue to increase after 8 h. The expression of autophagy-related factor genes is phase-specific. The LC3 gene is rapidly expressed up to 2 h, Beclin-1, ACTG7, and ACTG12 up to 4 h after death ( Martínez et al, 2019 ).…”

Section: Thanatotranscriptome and Dna/rna Changes Related To Postmortem Interval (Pmi)mentioning

confidence: 99%

Cadaveric Stem Cells: Their Research Potential and Limitations

Cieśla

Tomsia

2021

Front. Genet.

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In the era of growing interest in stem cells, the availability of donors for transplantation has become a problem. The isolation of embryonic and fetal cells raises ethical controversies, and the number of adult donors is deficient. Stem cells isolated from deceased donors, known as cadaveric stem cells (CaSCs), may alleviate this problem. So far, it was possible to isolate from deceased donors mesenchymal stem cells (MSCs), adipose delivered stem cells (ADSCs), neural stem cells (NSCs), retinal progenitor cells (RPCs), induced pluripotent stem cells (iPSCs), and hematopoietic stem cells (HSCs). Recent studies have shown that it is possible to collect and use CaSCs from cadavers, even these with an extended postmortem interval (PMI) provided proper storage conditions (like cadaver heparinization or liquid nitrogen storage) are maintained. The presented review summarizes the latest research on CaSCs and their current therapeutic applications. It describes the developments in thanatotranscriptome and scaffolding for cadaver cells, summarizes their potential applications in regenerative medicine, and lists their limitations, such as donor’s unknown medical condition in criminal cases, limited differentiation potential, higher risk of carcinogenesis, or changing DNA quality. Finally, the review underlines the need to develop procedures determining the safe CaSCs harvesting and use.

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“The big sleep: Elucidating the sequence of events in the first hours of death to determine the postmortem interval”

Cited by 7 publications

References 80 publications

A miRNome analysis at the early postmortem interval

A miRNome analysis at the early postmortem interval

Postmortem Interval Estimation: New Approaches by the Analysis of Human Tissues and Microbial Communities’ Changes

Cadaveric Stem Cells: Their Research Potential and Limitations

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