1994
DOI: 10.1111/j.1476-5381.1994.tb17132.x
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The binding of 1,3‐[3H]‐dipropyl‐8‐cyclopentylxanthine to adenosine Ai receptors in rat smooth muscle preparations

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Cited by 19 publications
(29 citation statements)
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“…The density of binding sites increased up to postnatal day 25, where peak levels were observed, and decreased thereafter to levels commensurate with those already established in the adult duodenum (Peachey et al, 1994). The KD values generated for [3H]-DPCPX binding sites in neonatal rat duodenum did not differ significantly from the KD value previously generated for adult rat duodenum and suggests that the observed differences in receptor density are not due to binding to another receptor system.…”
Section: Discussionmentioning
confidence: 51%
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“…The density of binding sites increased up to postnatal day 25, where peak levels were observed, and decreased thereafter to levels commensurate with those already established in the adult duodenum (Peachey et al, 1994). The KD values generated for [3H]-DPCPX binding sites in neonatal rat duodenum did not differ significantly from the KD value previously generated for adult rat duodenum and suggests that the observed differences in receptor density are not due to binding to another receptor system.…”
Section: Discussionmentioning
confidence: 51%
“…In view of this, it was not possible to differentiate clearly the development of the A1 and A2 receptors from the ontogenetic profile of the P1 purinoceptor already constructed, and indeed it was not known whether in neonatal rat duodenum the receptors are of the same subtype as those identified in the adult. Thus, this study attempted to follow the development of the Al receptor in the rat duodenum, using functional assays and the A1 receptor ligand binding assay method developed for adult rat duodenum (Peachey et al, 1994), and also the development of the A2 receptor by studying functional responses.…”
Section: Discussionmentioning
confidence: 99%
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“…The cell suspension was homogenized with a Polytron and the homogenate was centrifuged for 30 min at 18000 rpm. The membrane pellet was resuspended in 50 mM Tris-HCl buffer at pH 7.4 for A 1 50,51) were utilized in radioligand binding assay to membranes prepared from Chinese hamster ovary (CHO) cells expressing the appropriate adenosine receptors on their surfaces. 52) Adenosine-deaminase (ADA) was present during the preparation of the membranes, in a preincubation of 30 min at 37°C, and during the incubation with the radioligands.…”
Section: Methodsmentioning
confidence: 99%
“…To the preparation medium was added RO-20-1724 (4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinon) as phosphodiesterase-inhibitor and NECA (5-(6-amino-9H-purin-9-yl)-N-ethyl-3,4-dihydroxytetrahydrofuran-2-carboxamide) as non selective adenosine agonist. IC 50 values for concentration-dependent inhibition of NECA-stimulated adenylyl cyclase caused by antagonists were calculated according to Cheng and Prusoff equation. The protein concentration was determined according to a Bio-Rad method.…”
Section: Methodsmentioning
confidence: 99%