The Binding of Iodinated hCG to Porcine Granulosa Cells1

Kammerman, Sandra; Canfield, Robert E.; Kolena, J; Channing, Cornelia P.

doi:10.1210/endo-91-1-65

Cited by 76 publications

(23 citation statements)

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“…adrenal, kidney, heart and liver do not specifically bind l25l-HCG. The inhibition of binding of iodinated HCG to ova ries by unlabeled HCG as well as LH, but not FSH, has been thought to indicate that LH and HCG share similar receptor sites and that FSH is bound to separate receptor sites (10).…”

Section: Discussionmentioning

confidence: 99%

Ontogenic Development of the Responsiveness in cAMP Synthesis to LH and PGE<sub>1</sub> and Gonadotropin Receptors in the Rat Ovary

Kolena¹

1976

Neonatology

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Incubation of ovaries derived from rats aged 9, 13, 16, and 20 days with LH resulted in a significant rise in cAMP synthesis. The ovaries from 2- and 5-day-old rats were, however, unresponsive to stimulation by LH. By contrast, prostaglandin E₁ (PGE₁) evoked a sharp response in ovarian synthesis of cAMP even in 2-day-old rats. In order to determine why neonatal ovaries do not respond to LH, gonadotropic receptors have been studied. HCG binding sites were present in homogenates of untreated and gonadotropins primed rat ovaries. The specificity of this ¹²⁵I-HCG binding was demonstrated by the competition of unlabeled HCG and LH for binding sites and failure of such binding to occur in specimens obtained from control tissues. FSH, ACTH and growth hormone were not able to compete significantly for ¹²⁵I-HCG binding. The HCG receptor is present in ovaries during the whole postnatal period. The results suggest that the unresponsiveness of neonatal rat ovaries to LH is not caused by later maturation of gonadotropic receptors.

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Section: Discussionmentioning

confidence: 99%

Ontogenic Development of the Responsiveness in cAMP Synthesis to LH and PGE<sub>1</sub> and Gonadotropin Receptors in the Rat Ovary

Kolena¹

1976

Neonatology

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“…It is known that follicular cells have specific receptors for LH (Rajaniemi & Vanha-Perttula, 1972;Kammerman, Canfield, Kolena & Channing, 1972), 102 E. V. YoungLai which have been utilized for the assay of LH (Shirley & Stephenson, 1973 (Text-fig. 3).…”

Section: Discussionmentioning

confidence: 99%

Steroid Production by the Isolated Rabbit Ovarian Follicle

Ev¹

1974

Reproduction

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Summary. Graafian follicles from mature New Zealand white rabbits were incubated for 4 hr at 37\s=deg\Cwith air as the gas phase. The medium was changed every 15 min for 1 hr. Medium containing ovine LH was then added for four consecutive medium changes. Samples of the medium were analysed for 17\g=b\-hydroxyandrogens,oestrogens and progestins by radioimmunoassay procedures. When no LH was present, progestin and 17\g=b\-hydroxyandrogen secretion declined to negligible levels within 2 hr. Addition of 10 \g=m\g ovine LH to the medium for 1 hr caused an increase in 17\g=b\-hydroxyandrogen secretion which continued for the duration of the experiment. No significant change was observed in oestrogen secretion but there was a slight increase in progestin secretion. Addition of 5 \ g=m\ g and 1 \ g=m\ g LH for 15 min also caused an elevation of 17\ g=b\ \ x=r eq-\ hydroxyandrogen secretion within 1 hr whereas progestin secretion took longer to increase and no change in oestrogen secretion was observed. No effect was observed with LH concentrations of less than 500 pg/ml for 1 hr, but 1 and 10 ng LH/ml also caused an increase in 17\g=b\-hydroxyandrogen secretion. Oestrogen secretion was also stimulated at these levels. Control incubations with interstitial tissue showed no oestrogen secretion, slight secretion of 17\g=b\-hydroxyandrogens and greater secretion of progestin which was stimulated by exogenous LH.

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“…Channing (I 970a) showed that the properties of adult monkey granulosa cells change with the size of the follicle from which they are harvested; the cells originating from small follicles need both FSH and LH in order to luteinize, those from larger follicles luteinize with either FSH or LH. This last result was also observed with 4 to 6 monthold pigs when granulosa cells were harvested from 3-5 mm follicles (Channing 1970b) and cyclic AMP was shown to be involved in this phenomenon Seymor 1970, Kolena andChanning 1972 Fig. 3.…”

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confidence: 56%

“…However, contradictory results have been obtained. In the ease of the ovary, the only one eonsidered here, a stimulation of AC aetivity by FSH was observed in prepubertal rats (Fontaine, Fontaine-Bertrand, Salmon and Delerue Le Belle 1970) and in poreine granulosa cells (Kolena and Channing 1972) but not in rabbits (Dorrington and Bagget 1969). Speeies differences or physiological differences could explain trus diserepaney, Indeed preliminary experiments on prepubertal rats suggested that the sensitivity of ovarian AC to FSH varied with the age; in order to analyze this phenomenon we have followed the basal and stimulated AC in 14 to 31-day-old rats.…”

Section: Introductionmentioning

confidence: 69%

Age-Related Changes in FSH- and in LH-Sensitive Ovarian Adenyl Cyclase from Prepubertal Rats

Ya¹,

Salmon²,

Fontaine-Bertrand³

et al. 1973

Horm Metab Res

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FSH and LH stimulate adenyl eyclase (AC) activity in ovarian homogenates from 14 to 31-day-old rats. In presence of maximally aetive eoneentrations of LH, AC aetivity is maximal at 16-19 days of life and then deereases. In presence of maximally active concentrations of FSH, AC activity is relatively low in 14 to 16-day-old rats and then rises until a maximal value observed in 19 to 23-day-old rats. At this age the stimulation of the basal AC aetivity is higher with FSH (6 x) than with LH (3 x). Almost total additivity of the maximal aetions of FSH and LH is observed in 16 to 18-day-old rats; in older animals, when FSH is highly aetive, there is only a partial or no additivity. Concentrations of pure hormones giving half maximal stimulation are smaller for FSH than for LH.These results suggest that FSH, like LH, exerts at least so me of its physiological effeets by the intermediary of 3'5' cyclie AMP. They indicate that physiologieal changes at the level of reeeptor-adenyl cyclase systems can be important in regulating the response of target glands to stimulating hormones.

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The Binding of Iodinated hCG to Porcine Granulosa Cells1

Cited by 76 publications

References 0 publications

Ontogenic Development of the Responsiveness in cAMP Synthesis to LH and PGE<sub>1</sub> and Gonadotropin Receptors in the Rat Ovary

Ontogenic Development of the Responsiveness in cAMP Synthesis to LH and PGE<sub>1</sub> and Gonadotropin Receptors in the Rat Ovary

Steroid Production by the Isolated Rabbit Ovarian Follicle

Age-Related Changes in FSH- and in LH-Sensitive Ovarian Adenyl Cyclase from Prepubertal Rats

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