The bio-interface between functionalized Au NR@GO nanoplatforms with protein corona and their impact on delivery and release system

Assali, Akram; Razzazan, Shayan; Akhavan, Omid; Adeli, Mohsen; Atyabi, Fatemeh

doi:10.1016/j.colsurfb.2018.10.042

Cited by 35 publications

(19 citation statements)

References 28 publications

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“…The presence of this protein corona on the surface of nanoparticles has previously been shown to impact the cellular internalization of the delivery systems and the release of the entrapped drugs, modifying the biological response in vivo. 37 , 38 It is hoped that the surface modification of AuNCs with Lf, PEG and PEI could minimize protein adsorption and alter the corona composition, as a study by Assali et al 39 previously reported that functionalized gold nanoplatforms displayed less interactions with proteins and adsorbed less opsonins than their unmodified counterparts.…”

Section: Discussionmentioning

confidence: 99%

Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro

Almowalad¹,

Somani²,

Laskar³

et al. 2021

IJN

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Background Gold nanocages have been widely used as multifunctional platforms for drug and gene delivery, as well as photothermal agents for cancer therapy. However, their potential as gene delivery systems for cancer treatment has been reported in combination with chemotherapeutics and photothermal therapy, but not in isolation so far. The purpose of this work was to investigate whether the conjugation of gold nanocages with the cancer targeting ligand lactoferrin, polyethylene glycol and polyethylenimine could lead to enhanced transfection efficiency on prostate cancer cells in vitro, without assistance of external stimulation. Methods Novel lactoferrin-bearing gold nanocages conjugated to polyethylenimine and polyethylene glycol have been synthesized and characterized. Their transfection efficacy and cytotoxicity were assessed on PC-3 prostate cancer cell line following complexation with a plasmid DNA. Results Lactoferrin-bearing gold nanocages, alone or conjugated with polyethylenimine and polyethylene glycol, were able to condense DNA at conjugate:DNA weight ratios 5:1 and higher. Among all gold conjugates, the highest gene expression was obtained following treatment with gold complex conjugated with polyethylenimine and lactoferrin, at weight ratio 40:1, which was 1.71-fold higher than with polyethylenimine. This might be due to the increased DNA cellular uptake observed with this conjugate, by up to 8.65-fold in comparison with naked DNA. Conclusion Lactoferrin-bearing gold nanocages conjugates are highly promising gene delivery systems to prostate cancer cells.

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Section: Discussionmentioning

confidence: 99%

Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro

Almowalad¹,

Somani²,

Laskar³

et al. 2021

IJN

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“…259 In this regard, graphene-AuNPs hybrids have attracted a great deal of attention for various medical applications owing to their unique optoelectronic properties. [260][261][262] Graphene-AuNPs hybrids are used as biosensors for the detection and diagnosis of cancer cells, due to their prominent surface plasmon resonance (SPR), 263 NIR emission, quenching effect, adsorption of bioreceptors, enzyme mimic-peroxidase activities, improved electron transfer rate, and conductivity of both graphene and AuNPs. 264 Moreover, GO-AuNPs are reported as electrochemical immunosensors for detection of various cancer markers including CEA, CA125, P53, PSA, AFP, and Vascular endothelial growth factor (VEGF) 150.…”

Section: Graphene-metal Platformsmentioning

confidence: 99%

Functionalized Graphene Platforms for Anticancer Drug Delivery

Sattari¹,

Adeli²,

Beyranvand³

et al. 2021

IJN

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Two-dimensional nanomaterials are emerging as promising candidates for a wide range of biomedical applications including tissue engineering, biosensing, pathogen incapacitation, wound healing, and gene and drug delivery. Graphene, due to its high surface area, photothermal property, high loading capacity, and efficient cellular uptake, is at the forefront of these materials and plays a key role in this multidisciplinary research field. Poor water dispersibility and low functionality of graphene, however, hamper its hybridization into new nanostructures for future nanomedicine. Functionalization of graphene, either by covalent or non-covalent methods, is the most useful strategy to improve its dispersion in water and functionality as well as processability into new materials and devices. In this review, recent advances in functionalization of graphene derivatives by different (macro)molecules for future biomedical applications are reported and explained. In particular, hydrophilic functionalization of graphene and graphene oxide (GO) to improve their water dispersibility and physicochemical properties is discussed. We have focused on the anticancer drug delivery of polyfunctional graphene sheets.

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“…NPs function as antigen carriers [1], imaging reagents, molecular tags [2], therapeutic, diagnostic, and theranostic entities, and also as part of simultaneous delivery modules [3][4][5]. The multifunctional core-shell nanoplatforms made from gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) for miRNA delivery and their study on the delivery and release profile were reported [6,7]. Notably, as NPs are foreign to the body, macrophages, characterized by plasticity and heterogeneity [8], have to play crucial roles in in vivo situations to recognize, process, and clean-off NPs [9].…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity

et al. 2021

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Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly (p < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria Staphylococcus aureus and Escherichia coli. Under experimental conditions, upon bacterial exposure, the combined GNPs and GOFs induced significant clearance of bacteria through phagosome maturation (p < 0.0001) from time-points of 6 to 30 min and production of reactive oxygen species (ROS, p < 0.0001) through the NADPH oxidase 2 (NOX2, p < 0.0001)-based feedback mechanism. The effects of the combined presence of GNPs and GOFs on phagocytosis (p < 0.0001) suggested a synergistic action underway, also achieved through elevated signal transduction activity in the bone-marrow-derived macrophages (BMDM, p < 0.0001). The current study demonstrated that GNPs’ and GOFs’ bactericidal assisting potentials could be considered an effective and alternative strategy for treating infections from both positive and negative bacterial strains.

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The bio-interface between functionalized Au NR@GO nanoplatforms with protein corona and their impact on delivery and release system

Cited by 35 publications

References 28 publications

Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro

Lactoferrin-Bearing Gold Nanocages for Gene Delivery in Prostate Cancer Cells in vitro

Functionalized Graphene Platforms for Anticancer Drug Delivery

Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity

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