The Bio-SANS instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory

Heller, William T.; Urban, Volker S.; Lynn, Gary W.; Weiss, Kevin L.; O’Neill, Hugh; Pingali, Sai Venkatesh; Qian, Shuo; Littrell, Kenneth C.; Melnichenko, Yuri B.; Buchanan, Michelle V.; Selby, D.L.; Wignall, G. D.; Butler, Paul; Myles, Dean A. A.

doi:10.1107/s1600576714011285

Cited by 97 publications

(70 citation statements)

References 46 publications

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“…SANS measurements were conducted at the Bio-SANS instrument at the High-Flux Isotope Reactor, Oak Ridge National Laboratory (Heller et al, 2014). The three different instrument configurations employed to cover the range, 0.003 , Q (Å -1 ) , 0.7, of scattering vectors with sufficient overlap had sampleto-detector distances of 1.…”

Section: Sans Measurementsmentioning

confidence: 99%

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

et al. 2017

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At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ;4 cm of the stem. We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering. Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear. Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem. In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly. These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate. These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.

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Section: Sans Measurementsmentioning

confidence: 99%

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

et al. 2017

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“…at Oak Ridge National Laboratory (Heller et al, 2014). EQ-SANS was operated at a 30 Hz mode, which provided wavelength bands of 2.5 to 6.1 Å and 9.4 to 13.4 Å.…”

Section: Computational Modelingmentioning

confidence: 99%

A Structural Study of CESA1 Catalytic Domain of Arabidopsis Cellulose Synthesis Complex: Evidence for CESA Trimers

et al. 2015

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A cellulose synthesis complex with a "rosette" shape is responsible for synthesis of cellulose chains and their assembly into microfibrils within the cell walls of land plants and their charophyte algal progenitors. The number of cellulose synthase proteins in this large multisubunit transmembrane protein complex and the number of cellulose chains in a microfibril have been debated for many years. This work reports a low resolution structure of the catalytic domain of CESA1 from Arabidopsis (Arabidopsis thaliana; AtCESA1CatD) determined by small-angle scattering techniques and provides the first experimental evidence for the self-assembly of CESA into a stable trimer in solution. The catalytic domain was overexpressed in Escherichia coli, and using a two-step procedure, it was possible to isolate monomeric and trimeric forms of AtCESA1CatD. The conformation of monomeric and trimeric AtCESA1CatD proteins were studied using small-angle neutron scattering and small-angle x-ray scattering. A series of AtCESA1CatD trimer computational models were compared with the small-angle x-ray scattering trimer profile to explore the possible arrangement of the monomers in the trimers. Several candidate trimers were identified with monomers oriented such that the newly synthesized cellulose chains project toward the cell membrane. In these models, the class-specific region is found at the periphery of the complex, and the plant-conserved region forms the base of the trimer. This study strongly supports the "hexamer of trimers" model for the rosette cellulose synthesis complex that synthesizes an 18-chain cellulose microfibril as its fundamental product.

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“…SANS experiments were carried out using the Bio‐SANS instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory[32]. Scattering data were collected at sample‐to‐detector distances of 0.3 and 6 m to cover a Q range of 0.0067–0.73 Å −1 using 6 Å neutron beam ( q = (4 π / λ ) sin( θ /2), where λ is the neutron wavelength and θ is the scattering angle).…”

Section: Methodsmentioning

confidence: 99%

Oligomerization state and pigment binding strength of the peridinin‐Chla‐protein

Jiang

Zhang

et al. 2015

FEBS Letters

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a b s t r a c tThe peridinin-chlorophyll a-protein (PCP) is one of the major light harvesting complexes (LHCs) in photosynthetic dinoflagellates. We analyzed the oligomeric state of PCP isolated from the dinoflagellate Symbiodinium, which has received increasing attention in recent years because of its role in coral bleaching. Size-exclusion chromatography (SEC) and small angle neutron scattering (SANS) analysis indicated PCP exists as monomers. Native mass spectrometry (native MS) demonstrated two oligomeric states of PCP, with the monomeric PCP being dominant. The trimerization may not be necessary for PCP to function as a light-harvesting complex.

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The Bio-SANS instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory

Cited by 97 publications

References 46 publications

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

A Structural Study of CESA1 Catalytic Domain of Arabidopsis Cellulose Synthesis Complex: Evidence for CESA Trimers

Oligomerization state and pigment binding strength of the peridinin‐Chla‐protein

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