The Bioactive Polypyrrole/Polydopamine Nanowire Coating with Enhanced Osteogenic Differentiation Ability with Electrical Stimulation

He, Yuan; Dai, Lingfeng; Zhang, Xiuming; Sun, Yanan; Shi, Wei; Ge, Dongtao

doi:10.3390/coatings10121189

Cited by 16 publications

(9 citation statements)

References 39 publications

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“…MC3T3-E1 cells under the combined effect of electrical and mechanical stimulation. [33,122] However, the non-portability of external power sources and the infection caused by invasive electrodes limit the further application of external stimulation. [123] Furthermore, the applied homogeneous electrical stimulation does not mimic the electrical microenvironment of ECM in the normal physiological state.…”

Section: The Intrinsic Electrical Properties Of Semiconductive Hetero...mentioning

confidence: 99%

Semiconductive Biomaterials for Pathological Bone Repair and Regeneration

Fan,

Ran,

Wang

et al. 2024

Adv Funct Materials

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Bone implant biomaterials are among the most used materials for clinical application. Despite significant advances in biocompatibility and osteoconductivity, conventional biomaterials lack the ability to cope with the pathological microenvironment (inflammation, infection, residual tumors, etc.) during bone repair. Semiconductor implant materials have unique electrical, optical, ultrasound, and thermal response properties, which facilitate non‐invasively and controllably dynamic repair of pathological bone defects. In this review, the design and synthesis of a new generation of semiconductor‐driven bone implant materials are summarized, the mechanism of action of semiconductive biomaterials' functional interfaces and the dynamic repair process of bone tissues are discussed, and new strategies for the problems encountered during clinical osseointegration is provided. Finally, the review outlooks the future of functional semiconductive implants for bone defect repair.

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Section: The Intrinsic Electrical Properties Of Semiconductive Hetero...mentioning

confidence: 99%

Semiconductive Biomaterials for Pathological Bone Repair and Regeneration

Fan,

Ran,

Wang

et al. 2024

Adv Funct Materials

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“…Electrical stimulation (ES) can significantly promote the proliferation and differentiation of osteoblasts at the cellular and tissue level in a small amount of currents between 5 and 100 μA [ 108 , 109 ]. A representative example was carried out by He et al by using a continuous micro constant current electrical stimulation signal (10 µA) on the surface of different substrates (PPy NWs and PPy/PDA NWs) via an electrochemical workstation for 12 h after cells inoculation and for 2 h per day for 14 days [ 110 ]. It was noticeable that continuous ES increase the adhesion and proliferation of MC3T3-E1 cell in PPy/PDA NWs, significantly.…”

Section: Specific Improvement Strategies For Specific Body Partsmentioning

confidence: 99%

Conductive Polymeric-Based Electroactive Scaffolds for Tissue Engineering Applications: Current Progress and Challenges from Biomaterials and Manufacturing Perspectives

Marsudi

Ariski

Wibowo

et al. 2021

IJMS

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The practice of combining external stimulation therapy alongside stimuli-responsive bio-scaffolds has shown massive potential for tissue engineering applications. One promising example is the combination of electrical stimulation (ES) and electroactive scaffolds because ES could enhance cell adhesion and proliferation as well as modulating cellular specialization. Even though electroactive scaffolds have the potential to revolutionize the field of tissue engineering due to their ability to distribute ES directly to the target tissues, the development of effective electroactive scaffolds with specific properties remains a major issue in their practical uses. Conductive polymers (CPs) offer ease of modification that allows for tailoring the scaffold’s various properties, making them an attractive option for conductive component in electroactive scaffolds. This review provides an up-to-date narrative of the progress of CPs-based electroactive scaffolds and the challenge of their use in various tissue engineering applications from biomaterials perspectives. The general issues with CP-based scaffolds relevant to its application as electroactive scaffolds were discussed, followed by a more specific discussion in their applications for specific tissues, including bone, nerve, skin, skeletal muscle and cardiac muscle scaffolds. Furthermore, this review also highlighted the importance of the manufacturing process relative to the scaffold’s performance, with particular emphasis on additive manufacturing, and various strategies to overcome the CPs’ limitations in the development of electroactive scaffolds.

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“…The choice of the most appropriate technique depends on the physico-chemical properties of the implant on which functionalization is performed [35]. Since the biological inertia of the PPy surface reduces cell affinity and bioactivity [36], an alternative method of protein bio-functionalization was employed to improve the bioactivity of our implants coated with a PPy film: Fn electrodeposition.…”

Section: Introductionmentioning

confidence: 99%

Fibronectin Conformations after Electrodeposition onto 316L Stainless Steel Substrates Enhanced Early-Stage Osteoblasts’ Adhesion but Affected Their Behavior

Alfonsi,

Karunathasan,

Mamodaly-Samdjee

et al. 2023

JFB

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The implantation of metallic orthopedic prostheses is increasingly common due to an aging population and accidents. There is a real societal need to implement new metal implants that combine durability, good mechanical properties, excellent biocompatibility, as well as affordable costs. Since the functionalization of low-cost 316L stainless steel substrates through the successive electrodeposition of a polypyrrole film (PPy) and a calcium phosphate deposit doped with silicon was previously carried out by our labs, we have also developed a bio-functional coating by electrodepositing or oxidating of fibronectin (Fn) coating. Fn is an extracellular matrix glycoprotein involved in cell adhesion and differentiation. Impacts of either electrodeposition or oxidation on the structure and functionality of Fn were first studied. Thus, electrodeposition is the technique that permits the highest deposition of fibronectin, compared to adsorption or oxidation. Furthermore, electrodeposition seems to strongly modify Fn conformation by the formation of intermingled long fibers, resulting in changes to the accessibility of the molecular probes tested (antibodies directed against Fn whole molecule and Fn cell-binding domain). Then, the effects of either electrodeposited Fn or oxidized Fn were validated by the resulting pre-osteoblast behavior. Electrodeposition reduced pre-osteoblasts’ ability to remodel Fn coating on supports because of a partial modification of Fn conformation, which reduced accessibility to the cell-binding domain. Electrodeposited Fn also diminished α5 integrin secretion and clustering along the plasma membrane. However, the N-terminal extremity of Fn was not modified by electrodeposition as demonstrated by Staphylococcus aureus attachment after 3 h of culture on a specific domain localized in this region. Moreover, the number of pre-osteoblasts remains stable after 3 h culture on either adsorbed, oxidized, or electrodeposited Fn deposits. In contrast, mitochondrial activity and cell proliferation were significantly higher on adsorbed Fn compared with electrodeposited Fn after 48 h culture. Hence, electro-deposited Fn seems more favorable to pre-osteoblast early-stage behavior than during a longer culture of 24 h and 48 h. The electrodeposition of matrix proteins could be improved to maintain their bio-activity and to develop this promising, fast technique to bio-functionalize metallic implants.

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The Bioactive Polypyrrole/Polydopamine Nanowire Coating with Enhanced Osteogenic Differentiation Ability with Electrical Stimulation

Cited by 16 publications

References 39 publications

Semiconductive Biomaterials for Pathological Bone Repair and Regeneration

Semiconductive Biomaterials for Pathological Bone Repair and Regeneration

Conductive Polymeric-Based Electroactive Scaffolds for Tissue Engineering Applications: Current Progress and Challenges from Biomaterials and Manufacturing Perspectives

Fibronectin Conformations after Electrodeposition onto 316L Stainless Steel Substrates Enhanced Early-Stage Osteoblasts’ Adhesion but Affected Their Behavior

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