The bioavailability of sixteen tetracycline products

Meyer, Marvin C.; Dann, R.E.; Whyatt, Philip L.; Slywka, Gerald W.A.

doi:10.1007/bf01061403

Cited by 7 publications

(2 citation statements)

References 7 publications

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“…While it is not certain that the EDTA-extractable fraction of tetracyclines is fully bioavailable, it is a widely used indicator for estimating the bioavailability of tetracyclines in soils. 57,58 In fact, the significant correlations between the extracted tetracyclines measured by the HPLC and the biosensor method (Table S8) support the adequacy of this approach. The concentrations of EDTA-extractable tetracyclines measured by E. coli DH5α/ pMTmCherry were closer to those measured by the HPLC method than those measured by E. coli DH5α/pMTGFP (Table 2, Table S8, and Table S9), suggesting that the EDTAextractable tetracyclines using E. coli DH5α/pMTmCherry are a better indicator of the extractable and bioavailable fraction than that measured by E. coli DH5α/pMTGFP.…”

Section: Discussionmentioning

confidence: 65%

“…The unextractable tetracyclines in soils could be potentially bioactive but are difficult to measure using the whole-cell biosensors as they cannot be easily extracted, and other organic solvent extraction may need further cleanup (thus increasing analytical time and cost). While it is not certain that the EDTA-extractable fraction of tetracyclines is fully bioavailable, it is a widely used indicator for estimating the bioavailability of tetracyclines in soils. , In fact, the significant correlations between the extracted tetracyclines measured by the HPLC and the biosensor method (Table S8) support the adequacy of this approach. The concentrations of EDTA-extractable tetracyclines measured by E.…”

Section: Discussionmentioning

confidence: 98%

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A Fast and Easily Parallelizable Biosensor Method for Measuring Extractable Tetracyclines in Soils

Liu

Zhang

et al. 2019

Environ. Sci. Technol.

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Quantification of extractable antibiotics in soils is important to assessing their bioavailability and mobility, and ultimately their ecotoxicological and health risks. This study aimed to establish a biosensor method for detecting extractable tetracyclines in soils (Alfisol, Mollisol, and Ultisol) using whole-cell biosensors containing a reporter plasmid (pMTGFP or pMTmCherry) carrying fluorescent protein genes tightly controlled by tetracyclines-responsive control region (tetRO). This whole-cell biosensor method can simultaneously measure 96 or more samples within 6 h and is easily parallelizable, whereas a typical high-performance liquid chromatography (HPLC) method may require 7 times more of analysis time and much greater cost to achieve similar analytical throughput. The biosensor method had a detection limit for each of six tetracyclines between 5.32−10.2 μg/kg soil, which is considered adequate for detecting tetracyclines in ethylenediaminetetraacetic acid (EDTA) extracts of soils. Relative standard deviation was between 19.8−51.2% for the biosensor Escherichia coli DH5α/pMTGFP and 2.98−25.8% for E. coli DH5α/pMTmCherry, respectively, suggesting that E. coli DH5α/pMTmCherry was superior to E. coli DH5α/pMTGFP for detecting extractable tetracyclines in soils. This new, fast, easily parallelizable, and cost-effective biosensor method has the potential for measuring extractable concentrations of tetracyclines for a large number of soil samples in large-scale monitoring studies.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 98%

A Fast and Easily Parallelizable Biosensor Method for Measuring Extractable Tetracyclines in Soils

Liu

Zhang

et al. 2019

Environ. Sci. Technol.

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Influence of dioctyl sodium sulfosuccinate on the absorption of tetracycline

Shah

Hunt

Fairweather

et al. 1986

Biopharm & Drug Disp

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A pilot bioavailability study was carried out to evaluate the drug interaction and influence of dioctyl sodium sulfosuccinate (DS) on the absorption/bioavailability of tetracycline. Three tetracycline products--a fast dissolving capsule, a slow dissolving capsule and a suspension, were used in the study. DS was administered 30 minutes before tetracycline administration; and on -3, -2 and -1 day in the evening before tetracycline administration. Frequent urine samples were collected up to 48 hours and analyzed by a microbiological method. Although not statistically significant in this small study, the results suggest that there is a reduction in tetracycline bioavailability due to DS. The indiscriminate use of surface active agents to increase the dissolution rate of solid oral dosage forms in the belief that the resulting increased dissolution improves product bioavailability must be questioned.

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Predicting concentrations of trace organic compounds in municipal wastewater treatment plant sludge and biosolids using the PhATE™ model

Cunningham¹,

D’Aco

Pfeiffer

et al. 2012

Integr Envir Assess & Manag

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This article presents the capability expansion of the PhATE™ (pharmaceutical assessment and transport evaluation) model to predict concentrations of trace organics in sludges and biosolids from municipal wastewater treatment plants (WWTPs). PhATE was originally developed as an empirical model to estimate potential concentrations of active pharmaceutical ingredients (APIs) in US surface and drinking waters that could result from patient use of medicines. However, many compounds, including pharmaceuticals, are not completely transformed in WWTPs and remain in biosolids that may be applied to land as a soil amendment. This practice leads to concerns about potential exposures of people who may come into contact with amended soils and also about potential effects to plants and animals living in or contacting such soils. The model estimates the mass of API in WWTP influent based on the population served, the API per capita use, and the potential loss of the compound associated with human use (e.g., metabolism). The mass of API on the treated biosolids is then estimated based on partitioning to primary and secondary solids, potential loss due to biodegradation in secondary treatment (e.g., activated sludge), and potential loss during sludge treatment (e.g., aerobic digestion, anaerobic digestion, composting). Simulations using 2 surrogate compounds show that predicted environmental concentrations (PECs) generated by PhATE are in very good agreement with measured concentrations, i.e., well within 1 order of magnitude. Model simulations were then carried out for 18 APIs representing a broad range of chemical and use characteristics. These simulations yielded 4 categories of results: 1) PECs are in good agreement with measured data for 9 compounds with high analytical detection frequencies, 2) PECs are greater than measured data for 3 compounds with high analytical detection frequencies, possibly as a result of as yet unidentified depletion mechanisms, 3) PECs are less than analytical reporting limits for 5 compounds with low analytical detection frequencies, and 4) the PEC is greater than the analytical method reporting limit for 1 compound with a low analytical detection frequency, possibly again as a result of insufficient depletion data. Overall, these results demonstrate that PhATE has the potential to be a very useful tool in the evaluation of APIs in biosolids. Possible applications include: prioritizing APIs for assessment even in the absence of analytical methods; evaluating sludge processing scenarios to explore potential mitigation approaches; using in risk assessments; and developing realistic nationwide concentrations, because PECs can be represented as a cumulative probability distribution. Finally, comparison of PECs to measured concentrations can also be used to identify the need for fate studies of compounds of interest in biosolids.

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The bioavailability of sixteen tetracycline products

Cited by 7 publications

References 7 publications

A Fast and Easily Parallelizable Biosensor Method for Measuring Extractable Tetracyclines in Soils

A Fast and Easily Parallelizable Biosensor Method for Measuring Extractable Tetracyclines in Soils

Influence of dioctyl sodium sulfosuccinate on the absorption of tetracycline

Predicting concentrations of trace organic compounds in municipal wastewater treatment plant sludge and biosolids using the PhATE™ model

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