The Bioavailability of Vitamin C from Kiwifruit

Vissers, Margreet C.M.; Carr, Anitra C.; Pullar, Juliet M.; Bozonet, Stephanie M.

doi:10.1016/b978-0-12-394294-4.00007-9

Cited by 45 publications

(34 citation statements)

References 72 publications

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“…In mice implanted with CMT-93 cells, plasma and organ concentrations of ascorbate were significantly increased with increasing dietary supplementation (Table 1), reaching levels we have previously reported in non-tumor-bearing Gulo −/− mice 22. In mice injected with 10 6 CMT-93 cells, prior to complete regression, peak tumor growth was seen on day 13, with mice supplemented with 33 mg/L of ascorbate having greater tumor volumes (39±26 mm 3 ) than those supplemented with either 330 mg/L (7.6±6.1 mm 3 , P <0.05) or 3,300 mg/L (4.2±2 mm 3 , P <0.01) ascorbate (Figure 3A).…”

Section: Resultssupporting

confidence: 57%

“…Frozen tissues were homogenized to a fine powder in liquid nitrogen, weighed, and suspended in phosphate buffer, followed by measurement of DNA concentration to standardize for cell content, as described previously 19,22,23. The homogenate was extracted with perchloric acid containing EDTA for ascorbate measurements.…”

Section: Methodsmentioning

confidence: 99%

“…Ascorbate concentrations in cell lysates, tissues, and plasma were measured using reverse-phase high-performance liquid chromatography (HPLC; Waters 600 HPLC; Waters, MA, USA) with an ESA Coulochem II detector (Thermo Fisher Scientific), as described before 19,22,23. A fresh standard curve of 0–40 μM ascorbate was prepared for each HPLC run.…”

Section: Methodsmentioning

confidence: 99%

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Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo–/– mice

Campbell¹,

Vissers

Dachs³

2016

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In solid tumors, HIF1 upregulates the expression of hundreds of genes involved in cell survival, tumor growth, and adaptation to the hypoxic microenvironment. HIF1 stabilization and activity are suppressed by prolyl and asparagine hydroxylases, which require oxygen as a substrate and ascorbate as a cofactor. This has led us to hypothesize that intracellular ascorbate availability could modify the hypoxic HIF1 response and influence tumor growth. In this study, we investigated the effect of variable intracellular ascorbate levels on HIF1 induction in cancer cells in vitro, and on tumor-take rate and growth in the Gulo−/− mouse. These mice depend on dietary ascorbate, and were supplemented with 3,300 mg/L, 330 mg/L, or 33 mg/L ascorbate in their drinking water, resulting in saturating, medium, or low plasma and tissue ascorbate levels, respectively. In Lewis lung carcinoma cells (LL/2) in culture, optimal ascorbate supplementation reduced HIF1 accumulation under physiological but not pathological hypoxia. LL/2, B16-F10 melanoma, or CMT-93 colorectal cancer cells were implanted subcutaneously into Gulo−/− mice at a range of cell inocula. Establishment of B16-F10 tumors in mice supplemented with 3,300 mg/L ascorbate required an increased number of cancer cells to initiate tumor growth compared with the number of cells required in mice on suboptimal ascorbate intake. Elevated ascorbate intake was also associated with decreased tumor ascorbate levels and a reduction in HIF1α expression and transcriptional activity. Following initial growth, all CMT-93 tumors regressed spontaneously, but mice supplemented with 33 mg/L ascorbate had lower plasma ascorbate levels and grew larger tumors than optimally supplemented mice. The data from this study indicate that improved ascorbate intake is consistent with increased intracellular ascorbate levels, reduced HIF1 activity and reduced tumor initiation and growth, and this may be advantageous in the management of cancer.

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Section: Resultssupporting

confidence: 57%

Section: Methodsmentioning

confidence: 99%

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Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo–/– mice

Campbell¹,

Vissers

Dachs³

2016

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“…Therefore, alterations in both polyphenol and ASC levels might contribute to the observed impairment in seed development and viability. Interestingly, ASC bioavailability seems to be increased by high polyphenol concentration in plant-derived food; this feature further increases the putative nutritional value of these engineered crops (Vissers et al, 2013). …”

Section: Strategy Of Biofortificationmentioning

confidence: 99%

Strategies to increase vitamin C in plants: from plant defense perspective to food biofortification

2013

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Vitamin C participates in several physiological processes, among others, immune stimulation, synthesis of collagen, hormones, neurotransmitters, and iron absorption. Severe deficiency leads to scurvy, whereas a limited vitamin C intake causes general symptoms, such as increased susceptibility to infections, fatigue, insomnia, and weight loss. Surprisingly vitamin C deficiencies are spread in both developing and developed countries, with the latter actually trying to overcome this lack through dietary supplements and food fortification. Therefore new strategies aimed to increase vitamin C in food plants would be of interest to improve human health. Interestingly, plants are not only living bioreactors for vitamin C production in optimal growing conditions, but also they can increase their vitamin C content as consequence of stress conditions. An overview of the different approaches aimed at increasing vitamin C level in plant food is given. They include genotype selection by “classical” breeding, bio-engineering and changes of the agronomic conditions, on the basis of the emerging concepts that plant can enhance vitamin C synthesis as part of defense responses.

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“…최근에는 미국 식품의약국(FDA)이 규정한 20대 영양소를 골고루 함유하고 있고 특히, 암 예방과 변비해소 등에 대한 기능 성이 알려지면서 소비량이 계속 증가하고 있다 (Collins et al 2001;Collins et al 2003;Ferguson and Bollard 1990;Kim et al 2011 deliciosa를 대상으로 대과형(>80 g), 고비타민(>120 mg/100 g) 및 고당도(>14°Brix 이상)의 우량계통을 선발하였고 1990 년대 이후에야 Actinidia 종들간 교배육종을 통한 신품종 육성이 이루어지고 있다 (Kim et al 2007a(Kim et al , 2007bKim et al 2009 (Frédrique et al 1994;Kim et al 2003;Zhang et al 1983 (Shi et al 2010). 키위는 비타민 C (아스코르브산) 함 량이 매우 높은 과일로 알려져 있다 (Vissers et al 2013 (Crowhurst et al 2008). 대규모 (Crowhurst et al 1990), amplified fragment length polymorphisms (AFLPs) (Novo et al 2010;Testolin et al 2001;Xiao et al 1999), randomly amplified polymorphic DNAs (RAPDs) (Gill et al 1998;Huang et al 2002;Shirkot et al 2002), simple sequence repeats (SSRs) (Fraser et al 2004;Huang et al 1998;Korkovelos et al 2008;Man et al 2011), single nucleotide polymorphisms (SNPs) (Zhou et al 2011) 마커가 개발되어 왔다.…”

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Current status and prospects of kiwifruit (Actinidia chinensis) genomics

Kim

Joa³

et al. 2015

J Plant Biotechnol

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Kiwifruit is a new fruit crop that was commercialized in the late 1970s. Recently, its cultivation and consumption have increased rapidly worldwide. Kiwifruit is a dioecious, deciduous, and climbing plant having fruit with hairs and various flesh colors and a variation in ploidy level; however, the industry consists of very simple cultivars or genotypes. The need for efficient cultivar improvement together with the evolutional and biological perspectives based on unique plant characteristics, have recently encouraged genome analysis and bioinformatics application. The draft genome sequence and chloroplast genome sequence of kiwifruit were released in 2013 and 2015, respectively; and gene annotation has been in progress. Recently, transcriptome analysis has shifted from previous ESTs analysis to the RNA-seq platform for intensive exploration of controlled genetic expression and gene discovery involved in fruit ascorbic acid biosynthesis, flesh coloration, maturation, and vine bacterial canker tolerance. For improving conventional breeding efficiency, molecular marker development and genetic linkage map construction have advanced from basic approaches using RFLP, RAPD, and AFLP to the development of NGS-based SSR and SNP markers linked to agronomically important traits and the construction of highly saturated linkage maps. However, genome and transcriptome studies have been limited in Korea. In the near future, kiwifruit genome and transcriptome studies are expected to translate to the practical application of molecular breeding.

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The Bioavailability of Vitamin C from Kiwifruit

Cited by 45 publications

References 72 publications

Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo–/– mice

Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo–/– mice

Strategies to increase vitamin C in plants: from plant defense perspective to food biofortification

Current status and prospects of kiwifruit (Actinidia chinensis) genomics

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The Bioavailability of Vitamin C from Kiwifruit

Cited by 45 publications

References 72 publications

Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in&nbsp;Gulo&ndash;/&ndash;&nbsp;mice

Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in&nbsp;Gulo&ndash;/&ndash;&nbsp;mice

Strategies to increase vitamin C in plants: from plant defense perspective to food biofortification

Current status and prospects of kiwifruit (Actinidia chinensis) genomics

Contact Info

Product

Resources

About

Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo–/– mice

Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo–/– mice