The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces

Kollimada, Somanna A.; Senger, Fabrice; Vignaud, Timothée; Théry, Manuel; Blanchoin, Laurent; Kurzawa, Laëtitia

doi:10.1091/mbc.e21-03-0109

Cited by 9 publications

(7 citation statements)

References 75 publications

(169 reference statements)

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“…Notably, experimental results suggest that adhered area increases on stiffer substrates (e.g. [32] report adhered area increasing from 7% to 12% on 30kPa to 1MPa gels, see also [11, 33]). There is also a correlation between focal adhesion size and increased myosin activity e.g.…”

Section: Uniform and Isotropic Contractile Pressurementioning

confidence: 99%

“…The substrate strain energy W S is easily experimentally measured and is often reported as a measure of mechanical activity, see e.g. [11][12][13][14]. The active work done W CA is also clearly important as it can be conceptually linked to the energy that is required to drive the cell into its contractile state, with hard constraints on its possible size [15].…”

mentioning

confidence: 99%

“…Although changes in traction forces are typically measured in mechanostransduction studies, recent work suggests that substrate strain energy can be used as a measure for mechanical activity [11, 12]. It has been suggested that cells may respond to directly changes in substrate strain energy [13].…”

mentioning

confidence: 99%

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Cell strain energy costs of active control of contractility

Solowiej-Wedderburn¹,

Dunlop²

2022

Preprint

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Cell mechanosensing is implicated in the control of a broad range of cell behaviours, with cytoskeletal contractility a key component. Experimentally, it is observed that the contractility of the cell responds to increasing substrate stiffness, showing increased contractile force and changing the distribution of cytoskeletal elements. Here we show using a theoretical model of active cell contractility that upregulation of contractility need not be energetically expensive, especially when combined with changes in adhesion and contractile distribution. Indeed, we show that a feedback mechanism based on maintenance of strain energy would require an upregulation in contractile pressure on all but the softest substrates. We consider both the commonly reported substrate strain energy and active work done. We demonstrate substrate strain energy would select for the observed clustering of cell adhesions on stiffer substrates which also enable an upregulation of total contractile pressure; while localisation of contractility has the greatest impact on the internal work.

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Section: Uniform and Isotropic Contractile Pressurementioning

confidence: 99%

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confidence: 99%

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Cell strain energy costs of active control of contractility

Solowiej-Wedderburn¹,

Dunlop²

2022

Preprint

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“…In TFM, fluorescent beads embedded in the elastic substrate serve as fiducial markers that report on substrate deformations induced by cells. The confinement of cells within adhesive patterns on top of TFM substrates allows the estimation of traction forces as a function of cell shape, as well as the averaging over many cells to obtain more reliable conclusions. , Existing protocols however rely on immobilization of large ECM proteins. To test if our patterning approach with small molecular ligands was compatible with TFM, beads were encapsulated in 2 kPa pAAm hydrogels containing caged AEMA, patterned, and functionalized with α 5 β 1 integrin-selective peptidomimetics.…”

Section: Resultsmentioning

confidence: 99%

Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers

Missirlis

Baños

Lussier

et al. 2022

ACS Appl. Mater. Interfaces

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We here present a micropatterning strategy to introduce small molecules and ligands on patterns of arbitrary shapes on the surface of poly(acrylamide)-based hydrogels. The main advantages of the presented approach are the ease of use, the lack of need to prefabricate photomasks, the use of mild UV light and biocompatible bioconjugation chemistries, and the capacity to pattern low-molecular-weight ligands, such as peptides, peptidomimetics, or DNA fragments. To achieve the above, a monomer containing a caged amine (NVOC group) was co-polymerized in the hydrogel network; upon UV light illumination using a commercially available setup, primary amines were locally deprotected and served as reactive groups for further functionalization. Cell patterning on various cell adhesive ligands was demonstrated, with cells responding to a combination of pattern shape and substrate elasticity. The approach is compatible with standard traction force microscopy (TFM) experimentation and can further be extended to reference-free TFM.

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“…After cells initially adhere within the ring-barrier, cell migration and proliferation result in the formation of large, single colonies. During cell colony formation, the cell-substrate and cell-cell adhesion cooperate to organize actomyosin networks and maintain force transmission [31][32][33][34][35][36]. The ability of hPSCs to form colonies depends on the reorganization of the cytoskeleton, the contraction of actin filaments, cell-cell interactions, and the timely function of the regulatory protein [32,33].…”

Section: Discussionmentioning

confidence: 99%

An in vitro culture platform for studying the effect of collective cell migration on spatial self-organization within induced pluripotent stem cell colonies

Kim

Kuroda

et al. 2023

J Biol Eng

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Background Human induced pluripotent stem cells (hiPSCs) provide an in vitro system to identify the impact of cell behavior on the earliest stages of cell fate specification during human development. Here, we developed an hiPSC-based model to study the effect of collective cell migration in meso–endodermal lineage segregation and cell fate decisions through the control of space confinement using a detachable ring culture system. Results The actomyosin organization of cells at the edge of undifferentiated colonies formed in a ring barrier differed from that of the cells in the center of the colony. In addition, even in the absence of exogenous supplements, ectoderm, mesoderm, endoderm, and extraembryonic cells differentiated following the induction of collective cell migration at the colony edge by removing the ring-barrier. However, when collective cell migration was inhibited by blocking E-cadherin function, this fate decision within an hiPSC colony was altered to an ectodermal fate. Furthermore, the induction of collective cell migration at the colony edge using an endodermal induction media enhanced endodermal differentiation efficiency in association with cadherin switching, which is involved in the epithelial-mesenchymal transition. Conclusions Our findings suggest that collective cell migration can be an effective way to drive the segregation of mesoderm and endoderm lineages, and cell fate decisions of hiPSCs.

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The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces

Cited by 9 publications

References 75 publications

Cell strain energy costs of active control of contractility

Cell strain energy costs of active control of contractility

Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers

An in vitro culture platform for studying the effect of collective cell migration on spatial self-organization within induced pluripotent stem cell colonies

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