The biogenesis of multivesicular endosomes

Grüenberg, Jean; Stenmark, Harald

doi:10.1038/nrm1360

Cited by 642 publications

(637 citation statements)

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“…Although some receptors are recycled back to the cell surface, cargo destined to be degraded is sorted into the multivesicular body (MVB) (Hicke, 2001;Katzmann et al, 2002). Cargo proteins concentrated on the limiting membranes of the MVB are internalized by invagination, and the membrane is pinched off to form intraluminal vesicles (Gruenberg and Stenmark, 2004). Thus, MVB sorting of proteins exposes them to degradation by lysosomal enzymes.…”

Section: Introductionmentioning

confidence: 99%

“…Close to the final step of sorting, the ESCRT-III complex, which is composed of CHMP1, CHMP2, CHMP3, CHMP4, CHMP5, and CHMP6, concentrates the cargos on the maturing endosomes (Raiborg et al, 2003;Gruenberg and Stenmark, 2004;Morita and Sundquist, 2004). Finally, additional class E VPS proteins, Vps4A and Vps4B, dissociate the ESCRT complex from the endosomal membrane in an ATP-dependent manner, allowing the cargo to be invaginated into the MVB (Babst et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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AMSH, an ESCRT-III Associated Enzyme, Deubiquitinates Cargo on MVB/Late Endosomes

Kyuuma

Kikuchi

Kojima

et al. 2006

Cell Struct. Funct.

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and 6 These two authors contributed equally to this work.ABSTRACT. The appropriate sorting of vesicular cargo, including cell-surface proteins, is critical for many cellular functions. Ubiquitinated cargo is targeted to endosomes and digested by lysosomal enzymes. We previously identified AMSH, a deubiquitination enzyme (DUB), to be involved in vesicular transport. Here, we purified an AMSH-binding protein, CHMP3, which is an ESCRT-III subunit. ESCRT-III functions on maturing endosomes, indicating AMSH might also play a role in MVB/late endosomes. Expression of an AMSH mutant lacking CHMP3-binding ability resulted in aberrant endosomes with accumulations of ubiquitinated cargo. Nevertheless, CHMP3-binding capability was not essential for AMSH's in vitro DUB activity or its endosomal localization, suggesting that, in vivo, the deubiquitination of endosomal cargo is CHMP3-dependent. Ubiquitinated cargo also accumulated on endosomes when catalytically inactive AMSH was expressed or AMSH was depleted. These results suggest that both the DUB activity of AMSH and its CHMP3-binding ability are required to clear ubiquitinated cargo from endosomes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

AMSH, an ESCRT-III Associated Enzyme, Deubiquitinates Cargo on MVB/Late Endosomes

Kyuuma

Kikuchi

Kojima

et al. 2006

Cell Struct. Funct.

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“…[65][66][67][68] The multivesicular phenotype can be explained by the transport of MHC class II molecules to multivesicular endosomes, which are abundant in the endocytic pathway. 69 The multilaminar, 'onion-like' phenotype of MIICs, however, has remained more enigmatic. It is tempting to speculated that MIICs obtain their multilaminar phenotype by fusion of MHC class II-containing endosomes with autophagosomes, given the fact that autophagosomes are delineated by a double membrane and often contain internal membrane sheets.…”

Section: Transport Of Autophagosomes To Mhc Class II Loading Compartmmentioning

confidence: 99%

Immune surveillance of intracellular pathogens via autophagy

Schmid

Münz

2005

Cell Death Differ

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MHC class II molecules are thought to present peptides derived from extracellular proteins to CD4 þ T cells, which are important mediators of adaptive immunity to infections. In contrast, autophagy delivers constitutively cytosolic material for lysosomal degradation and has so far been recognized as an efficient mechanism of innate immunity against bacteria and viruses. Recent studies, however, link these two pathways and suggest that intracellular cytosolic and nuclear antigens are processed for MHC class II presentation after autophagy.

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“…Les compartiments membranaires de la voie de dégradation, ECV et endosomes tardifs, sont, contrairement aux autres organites de l'appareil vacuolaire, très riches en vésicules internes. Ces derniè-res se forment au niveau des endosomes précoces où les molécules destinées à être dégradées y sont internalisées [1]. Depuis quelques années, de plus en plus d'exemples montrent que, dans les cellules de mammifère, les molécules incorporées dans les membranes internes ne sont pas forcément toutes dégradées.…”

Section: L'endocytose Cheval De Troie Pour L'infection Viraleunclassified

“…Le LBPA et Alix pourraient donc être responsable de l'invagination de la membrane limitante. Le PtdIns(3)P est lui surtout présent dans les endosomes précoces où il a été décrit comme étant nécessaire à la formation des ECV et au tri des molé-cules dans les membranes internes [1]. …”

Section: L'endocytose Cheval De Troie Pour L'infection Viraleunclassified

L’endocytose, cheval de Troie pour l’infection virale

Luyet

Grüenberg

2005

Med Sci (Paris)

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NOUVELLES MAGAZINE 909M/S n° 11, vol. 21, novembre 2005 d'une classification des deux sexes en deux espèces différentes (et pour la première fois d'une espèce dont tous les individus seraient des mâles), puisque mâles et femelles ont des trajectoires évolutives séparées et n'ont pas de descendance fertile [9]. Dans ce système, les deux sexes demeurent affiliés non pas par des échan-ges de gènes mais par une surprenante exploitation parasitique des femelles par les mâles et par la production en commun d'ouvrières stériles assurant le fonctionnement de la colonie. ◊

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The biogenesis of multivesicular endosomes

Cited by 642 publications

References 73 publications

AMSH, an ESCRT-III Associated Enzyme, Deubiquitinates Cargo on MVB/Late Endosomes

AMSH, an ESCRT-III Associated Enzyme, Deubiquitinates Cargo on MVB/Late Endosomes

Immune surveillance of intracellular pathogens via autophagy

L’endocytose, cheval de Troie pour l’infection virale

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