Nonglacial deposits of Middle Wisconsin age are being discovered with increased frequency across a broad region of southern Ontario, Canada, and provide strong evidence for a time of significant ice withdrawal from the lower Great Lakes region. With each new discovery, a refined understanding of regional climatic and paleoecological environments is emerging. In this paper, we present the results of a sedimentological and paleoecological study of a subtill organic deposit in Zorra Township, southwestern Ontario. The organic deposit, which lies beneath Nissouri Phase Catfish Creek Till (Late Wisconsin), has been dated by accelerator mass spectrometry at between 50.5 and 42.9 14 C ka BP. The organic remains are contained within slack water pond deposits infilling a channel incised into till either of Early Wisconsin or Illinoian age. The fossil assemblage appears to be strongly influenced by taphonomic processes, including degradation due to oxidation, bacterial and fungal decay, and glacial overriding. Reworking and (or) recycling and selective sorting as well as long-distance transport has also influenced the composition of the fossil assemblage preserved. Nonetheless, meaningful paleoecological information is still obtained from this record. Collectively, the pollen and plant macrofossils indicate a boreal-type pine-spruce forest with temperatures cooler than present. The absence of arctic tundra plants, as are found in many other deposits of similar age in the lower Great Lakes basin, is notable. A pond or wetland inhabited by shoreline herbs, shrubs, and trees was present at or proximal to the site. The freshwater mollusc and ostracode assemblages are consistent with a shallow water habitat with dense submerged vegetation. The terrestrial mollusc assemblage suggests a taiga or transitional taiga-tundra fauna. Together, these fossil groups provide one of the most comprehensive environmental reconstructions of Middle Wisconsin time (oxygen isotope stage 3 or OIS3) in southern Ontario and serve to build on the ever-increasing database of paleoecological information accumulating for this episode of the late Quaternary.Résumé : Des dépôts non glaciaires d'âge wisconsinien moyen sont découverts de plus en plus fréquemment dans une vaste région du sud de l'Ontario (Canada) et fournissent des indices probants d'une période de retrait important des glaces de la région du bassin inférieur des Grands Lacs. Chaque nouvelle découverte permet une compréhension plus pointue des milieux climatiques et paléoécologiques régionaux. Nous présentons les résultats d'une étude sédimentologique et paléoé-cologique d'un dépôt organique qui sous-tend un till dans le canton de Zorra, dans le sud-ouest de l'Ontario. La datation par spectrométrie de masse par accélérateur du dépôt organique, qui sous-tend le till de Catfish Creek de la phase de Nissouri (Wisconsinien tardif), a donné des âges entre 50,5 et 42,9 14 C ka BP. Les restes organiques sont contenus dans des dépôts d'étang d'eau stagnante qui remplissent un chenal creusé dans du t...