The biological activities of (1,3)-(1,6)-β-d-glucan and porous electrospun PLGA membranes containing β-glucan in human dermal fibroblasts and adipose tissue-derived stem cells

Woo, Yeon I.; Park, Bong Joo; Kim, Hye Lee; Lee, Mi Hee; Kim, Jung-Sung; Yang, Young Il; Kim, Jung Koo; Tsubaki, Kazufumi; Han, Dong‐Wook; Park, Jong Chul

doi:10.1088/1748-6041/5/4/044109

Cited by 42 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The use of glucans as a scaffold system for skin engineering has not been well‐studied. Poly(lactide ‐co‐ glycolide) scaffolds containing β ‐glucans were highly biocompatible with human fibroblasts and adipose‐derived stem cells,39 but these cell‐scaffold constructs were only evaluated in vitro. Gelatin/glucan scaffolds seeded with both human keratinocytes and fibroblasts improved full thickness wound healing in immunocompromised mice,40 demonstrating the potential of glucan biomaterials for skin regeneration, albeit in a minimally inflammatory setting.…”

Section: Discussionmentioning

confidence: 99%

Pullulan Hydrogels Improve Mesenchymal Stem Cell Delivery into High‐Oxidative‐Stress Wounds

Wong

Rustad

Glotzbach

et al. 2011

Macromolecular Bioscience

View full text Add to dashboard Cite

Cell-based therapies for wound repair are limited by inefficient delivery systems that fail to protect cells from the acute inflammatory environment. Here, a biomimetic hydrogel system is described that is based on the polymer pullulan, a carbohydrate glucan known to exhibit potent antioxidant capabilities. It is shown that pullulan hydrogels are an effective cell delivery system and improve mesenchymal stem cell survival and engraftment in high-oxidative-stress environments. The results suggest that glucan hydrogel systems may prove beneficial for progenitor-cell-based approaches to skin regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Pullulan Hydrogels Improve Mesenchymal Stem Cell Delivery into High‐Oxidative‐Stress Wounds

Wong

Rustad

Glotzbach

et al. 2011

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…After removal of the remaining MTT dye 200 µl of dimethylsulfoxide (DMSO) were added to solubilize the formazan crystals. Finally the optical densities (OD) at 570 nm were measured using an ELISA reader/spectrophotometer [36], with background subtraction at 630–690 nm. Ten parallel experiments were performed.…”

Section: Methodsmentioning

confidence: 99%

Effect of Bioglass on Growth and Biomineralization of SaOS-2 Cells in Hydrogel after 3D Cell Bioprinting

et al. 2014

View full text Add to dashboard Cite

We investigated the effect of bioglass (bioactive glass) on growth and mineralization of bone-related SaOS-2 cells, encapsulated into a printable and biodegradable alginate/gelatine hydrogel. The hydrogel was supplemented either with polyphosphate (polyP), administered as polyP•Ca2+-complex, or silica, or as biosilica that had been enzymatically prepared from ortho-silicate by silicatein. These hydrogels, together with SaOS-2 cells, were bioprinted to computer-designed scaffolds. The results revealed that bioglass (nano)particles, with a size of 55 nm and a molar ratio of SiO2∶CaO∶P2O5 of 55∶40∶5, did not affect the growth of the encapsulated cells. If silica, biosilica, or polyP•Ca2+-complex is co-added to the cell-containing alginate/gelatin hydrogel the growth behavior of the cells is not changed. Addition of 5 mg/ml of bioglass particles to this hydrogel significantly enhanced the potency of the entrapped SaOS-2 cells to mineralize. If compared with the extent of the cells to form mineral deposits in the absence of bioglass, the cells exposed to bioglass together with 100 µmoles/L polyP•Ca2+-complex increased their mineralization activity from 2.1- to 3.9-fold, or with 50 µmoles/L silica from 1.8- to 2.9-fold, or with 50 µmoles/L biosilica from 2.7- to 4.8-fold or with the two components together (100 µmoles/L polyP•Ca2+-complex and 50 µmoles/L biosilica) from 4.1- to 6.8-fold. Element analysis by EDX spectrometry of the mineral nodules formed by SaOS-2 revealed an accumulation of O, P, Ca and C, indicating that the mineral deposits contain, besides Ca-phosphate also Ca-carbonate. The results show that bioglass added to alginate/gelatin hydrogel increases the proliferation and mineralization of bioprinted SaOS-2 cells. We conclude that the development of cell-containing scaffolds consisting of a bioprintable, solid and cell-compatible inner matrix surrounded by a printable hard and flexible outer matrix containing bioglass, provide a suitable strategy for the fabrication of morphogenetically active and biodegradable implants.

show abstract

“…Taken together, our results contribute to the body of literature showing that PLGA surfaces can be successfully modified with components of the ECM, particularly collagen and GAG derivatives, for tissue‐engineering purposes and regenerative approaches (Adamczak et al ., ; Kumbar et al ., ; Shen et al ., ; Woo et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Artificial extracellular matrices support cell growth and matrix synthesis of human dermal fibroblasts in macroporous 3D scaffolds

Smissen

Hoffmeister

Friedrich

et al. 2015

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Surface modification of materials designed for regenerative medicine may improve biocompatibility and functionality. The application of glycosaminoglycans (GAGs) and chemically sulphated GAG derivatives is a promising approach for designing functional biomaterials, since GAGs interact with cell-derived growth factors and have been shown to support fibroblast growth in two-dimensional (2D) cultures. Here, coatings with artificial extracellular matrix (aECM), consisting of the structural protein collagen I and the GAG hyaluronan (HA) or sulphated HA derivatives, were investigated for their applicability in a three-dimensional (3D) system. As a model, macroporous poly(lactic-co-glycolic acid) (PLGA) scaffolds were homogeneously coated with aECM. The resulting scaffolds were characterized by compressive moduli of 0.9-1.2 MPa and pore sizes of 40-420 µm. Human dermal fibroblasts (dFbs) colonized these aECM-coated PLGA scaffolds to a depth of 400 µm within 14 days. In aECM-coated scaffolds, collagen I(α1) and collagen III(α1) mRNA expression was reduced, while matrix metalloproteinase-1 (MMP-1) mRNA expression was increased within 7 days, suggesting matrix-degradation processes. Stimulation with TGFβ1 generally increased cell density and collagen synthesis, demonstrating the efficiency of bioactive molecules in this 3D model. Thus, aECM with sulphated HA may modulate the effectivity of TGFβ1-induced collagen I(α1) expression, as demonstrated previously in 2D systems. Overall, the tested aECM with modified HA is also a suitable material for fibroblast growth under 3D conditions. Copyright © 2015 John Wiley & Sons, Ltd.

show abstract

The biological activities of (1,3)-(1,6)-β-d-glucan and porous electrospun PLGA membranes containing β-glucan in human dermal fibroblasts and adipose tissue-derived stem cells

Cited by 42 publications

References 26 publications

Pullulan Hydrogels Improve Mesenchymal Stem Cell Delivery into High‐Oxidative‐Stress Wounds

Pullulan Hydrogels Improve Mesenchymal Stem Cell Delivery into High‐Oxidative‐Stress Wounds

Effect of Bioglass on Growth and Biomineralization of SaOS-2 Cells in Hydrogel after 3D Cell Bioprinting

Artificial extracellular matrices support cell growth and matrix synthesis of human dermal fibroblasts in macroporous 3D scaffolds

Contact Info

Product

Resources

About