The biological activities of Arabis alpina L. subsp. brevifolia (DC.) Cullen against food pathogens

Abstract

In this study, in vitro biological activities of both methanol and ethanol extracts of Arabis alpina subsp. brevifolia were investigated. Also, the phenolic components of this plant was examined in this study. The extracts were tested against the eight strains of food pathogens for their antimicrobial activities by utilizing minimum inhibitory concentration (MIC) and disc diffusion assay. The non-enzymatic antioxidant activities were determined according to scavenging of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The phenolic compounds were analyzed by high performance liquid chromatography (HPLC). The main component was ellagic acid for the methanol extract of stem-leaf, rutin for the ethanol extract of stem-leaf, and 2,5-dihydroxybenzoic acid for the methanol and ethanol extracts of fruit-flower. The ethanolic extracts of leaves revealed antibacterial activities against Salmonella Typhimurium (7 mm) while the ethanolic extracts of flowers demonstrated no activity against the test pathogens. The methanolic extracts of leaf-flower showed antibacterial activities against S. Typhimurium (7 mm). No activity was observed against C. albicans. The MIC value for four test bacteria was 13000 μg/mL. The ethanol extracts of A. alpina subsp. brevifolia exhibited the highest DPPH inhibition (76%). This study showed that A. alpina subsp. brevifolia possesses antioxidant and antimicrobial activities.

The Influence of Selected Plant Essential Oils on Morphological and Physiological Characteristics in Pseudomonas Orientalis

“…Contemporary food market trends, associated with consumer expectations, are directed at natural products of high quality, low processing and long shelf-life [4,5]. The current fashion for healthy nutrition and care for the natural environment make it necessary to replace chemical preservatives with naturally-formed ones [6,7,8].…”

Isolation and Identification of Antioxidant Constituents From Extracellular Metabolites of Lactobacillus Brevis

“…Balpinar reported that methanol and ethanol extracts of A. alpina L. subsp. brevifolia exhibited antimicrobial activity against S. typhimurium (Balpinar, 2018). In that study extracts had no activity against Bacillus subtilis, Staphylococcus aureus, Salmonella Typhimurium, Enterococcus faecalis, Escherichia coli, Listeria monocytogenes, and the fungus Candida albicans.…”

“…There are many species in the Brassicaceae family that are used in traditional medicine and foods these are also recognized as the functional food. Brassicaceae family has received a great deal of attention in recent years because of its antioxidative and antimicrobial properties (Balpinar, 2018). The main metabolite of Brassicaceae is glucosinolates that contain sulfur.…”

“…brevifolia against different food pathogens were investigated. The extracts were shown to have polyphenols, such as quercetin and rutin, and phenolic acids, such as 2,5-dihydroxybenzoic, vanillic and caffeic acid (Balpinar, 2018).…”

“…Balpinar determined the highest DPPH scavenging activity (76.3%) in the flower-fruit-seed ethanol extract of A. alpina L. subsp. brevifolia (Balpinar, 2018). Due to both the use of a different species, difference of composition of flower-fruit-seed and difference in concentrations of samples and DPPH solution, %DPPH scavenging results of A. alpina L. subsp.…”