The biological effects of hsa-miR-1908 in human adipocytes

Yang, Lei; Shi, Chunmei; Chen, Ling; Pang, Lei; Xu, Gao; Gu, Nan; Zhu, Lijun; Guo, Xirong; Ni, Yuhui; Ji, Chenbo

doi:10.1007/s11033-014-3830-1

Cited by 23 publications

(27 citation statements)

References 36 publications

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“…Human pre-adipocytes from visceral (omental) adipose were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA). Pre-adipocytes were maintained and induced to differentiate as previously described ( 23 ). Briefly, pre-adipocyte differentiation medium (cat.…”

Section: Methodsmentioning

confidence: 99%

Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines

You

Shi

et al. 2016

Molecular Medicine Reports

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Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity-associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity-associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR-199a-3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR-199a-3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL-6 and leptin significantly induced miR-199a-3p expression in mature human adipocytes, while resistin had the opposite effect. miR-199a-3p may represent a factor in the modulation of obesity-associated IR and inflammatory responses.

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Section: Methodsmentioning

confidence: 99%

Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines

You

Shi

et al. 2016

Molecular Medicine Reports

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“…Moreover, ASCs have served as a tool to investigate the role of different genes associated with adipocyte metabolism [ 99 , 100 ]. Finally, ASCs have also been used for the study of miRNAs [ 1 , 101 ] and browning, because they are capable of converting from white to brown adipocytes [ 102 ], which enables the study of the differential effect of certain molecules in white and brown adipogenesis such as p53 [ 103 ].…”

Section: Human Cell Modelsmentioning

confidence: 99%

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Ruiz‐Ojeda

Rupérez

Gómez-Llorente

et al. 2016

IJMS

302

221

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Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue.

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“…Yu et al [13] demonstrated that peroxisome proliferator activated receptor-γ (PPAR-γ) agonist stimulation has in the subcutaneous and visceral fat depots distinct but overlapping effects on the expression of miRNAs that target the transforming growth factor-β (TGF-β), insulin and Wnt/β-catenin signaling pathways with key roles in the control of adipogenesis. Moreover, in vitro experiments with preadipocyte/adipocyte cultures have provided evidence for functional roles of a number of miRNAs in adipogenesis: miR-103, miR-320, and miR-1908 enhance adipogenesis while miR-146 and miR-194 decrease adipocyte differentiation [9,[14][15][16][17][18]. Of note, correlation was shown between dysregulation of miRNAs targeting white adipose tissue adipocytokine expression and non-alcoholic steatohepatitis [11], consistent with the hypothesis that adipocytokines regulated by miRNAs and secreted by adipose tissue impact the pathogenesis of non-alcoholic fatty liver disease.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-192* impairs adipocyte triglyceride storage

Mysore

Zhou

Sädevirta

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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We investigated the expression of miR-192* (miR-192-3p) in the visceral adipose tissue (VAT) of obese subjects and its function in cultured human adipocytes. This miRNA is a 3' arm derived from the same pre-miRNA as miR-192 (miR-192-5p) implicated in type 2 diabetes, liver disease and cancers, and is predicted to target key genes in lipid metabolism. In morbidly obese subjects undergoing bariatric surgery preceded by a very low calorie diet, miR-192* in VAT correlated negatively (r=-0.387; p=0.046) with serum triglyceride (TG) and positively with high-density lipoprotein (HDL) concentration (r=0.396; p=0.041). In a less obese patient cohort, the miRNA correlated negatively with the body mass index (r=-0.537; p=0.026). To characterize the function of miR-192*, we overexpressed it in cultured adipocytes and analyzed the expression of adipogenic differentiation markers as well as cellular TG content. Reduced TG and expression of the adipocyte marker proteins aP2 (adipocyte protein 2) and perilipin 1 were observed. The function of miR-192* was further investigated by transcriptomic profiling of adipocytes expressing this miRNA, revealing impacts on key lipogenic genes. A number of the mRNA alterations were validated by qPCR. Western analysis confirmed a marked reduction of the lipogenic enzyme SCD (stearoyl coenzyme A desaturase-1), the fatty aldehyde dehydrogenase ALDH3A2 (aldehyde dehydrogenase 3 family member A2) and the high-density lipoprotein receptor SCARB1 (scavenger receptor B, type I). SCD and ALDH3A2 were demonstrated to be direct targets of miR-192*. To conclude, the present data identify miR-192* as a novel controller of adipocyte differentiation and lipid homeostasis.

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The biological effects of hsa-miR-1908 in human adipocytes

Cited by 23 publications

References 36 publications

Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines

Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

MicroRNA-192* impairs adipocyte triglyceride storage

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