The biological functions and related signaling pathways of SPON2

Zhang, Jingrun; Liu, Ge; Liu, Yuchen; Yang, Pei; Xie, Junyuan; Wei, Xiaowei

doi:10.3389/fonc.2023.1323744

Front. Oncol.

2024

DOI: 10.3389/fonc.2023.1323744

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The biological functions and related signaling pathways of SPON2

Jingrun Zhang,

Ge Liu,

Yuchen Liu

et al.

Abstract: Spondin-2 (SPON2), also referred to as M-spondin or DIL-1, is a member of the extracellular matrix protein family known as Mindin-F-spondin (FS). SPON2 can be used as a broad-spectrum tumor marker for more than a dozen tumors, mainly prostate cancer. Meanwhile, SPON2 is also a potential biomarker for the diagnosis of certain non-tumor diseases. Additionally, SPON2 plays a pivotal role in regulating tumor metastasis and progression. In normal tissues, SPON2 has a variety of biological functions represented by p… Show more

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Cited by 2 publications

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AggressiveKRASmutations direct TGF-β response towards partial EMT in patient-derived colorectal cancer tumoroids

Mair,

König,

Mijović

et al. 2024

Preprint

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Transforming growth factor beta (TGF-β) exhibits complex and context-dependent cellular responses. While it mostly induces tumor-suppressive effects in early stages of tumorigenesis, its tumor promoting properties are evident in advanced disease. This TGF-β duality is still not fully understood, and whether TGF-β supports invasion and metastasis by influencing cancer cells directly, or rather through the stromal tumor compartment remains a matter of debate. Here, we utilized a library of colorectal cancer (CRC) patient-derived tumoroids (PDTs), representing a spectrum of tumor stages, to study cancer cell-specific responses to TGF-β. Using medium conditions allowing for the differentiation of PDTs, we observed TGF-β induced tumor-suppressive effects in early-stage tumoroids. PDTs with TGF-β pathway mutations or PDTs derived from metastatic tumors were insensitive to the treatment. Notably, one tumoroid line harboring an atypicalKRASQ22Kmutation underwent partial epithelial-to-mesenchymal transition (EMT), associated with morphological changes and increased invasiveness. On a molecular level, this was accompanied by elevated expression of mesenchymal genes, as well as deregulation of pathways associated with matrix remodeling and cell adhesion. Our results suggest that tumor cell intrinsic responses to TGF-β are critical in determining its tumor-suppressive or -promoting effects.

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AggressiveKRASmutations direct TGF-β response towards partial EMT in patient-derived colorectal cancer tumoroids

Mair,

König,

Mijović

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

A spatiotemporal atlas of human spermatogenesis based on single-cell transcriptomics and multiplex antibody imaging

Hikmet,

Méar,

Gustavsson

et al. 2024

Preprint

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Human health depends on complex processes of intercellular interactions and single-cell type-specific functions. Dramatic improvements in single-cell RNA sequencing (scRNA-seq) have opened up the possibility to identify functional states of single cells. Still, spatial methods are crucial for under-standing transcript location in relation to tissue architecture. Here, we con-structed a large-scale mapping strategy for proteins based on scRNA-seq and fluorescent-based multiplex immunohistochemistry (mIHC). We focused on the spatiotemporal landscape of 12 distinct germ cell states in human testis and performed an in-depth characterization of ~500 germ cell proteins. Quantitative spatial localization data based on a custom-built image analysis pipeline allowed us to cluster proteins according to expression, forming the basis for functional analysis. mRNA and protein expression dynamics showed multiple cases with low levels of co-expression, highlighting the necessity of studying protein levels in single-cell mapping projects. The presented workflow holds promise for proteome-wide tissue studies in health and disease.

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The biological functions and related signaling pathways of SPON2

Cited by 2 publications

References 104 publications

AggressiveKRASmutations direct TGF-β response towards partial EMT in patient-derived colorectal cancer tumoroids

AggressiveKRASmutations direct TGF-β response towards partial EMT in patient-derived colorectal cancer tumoroids

A spatiotemporal atlas of human spermatogenesis based on single-cell transcriptomics and multiplex antibody imaging

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