The Biological Mechanisms of Action of Cardiac Progenitor Cell Therapy

Pagano, Francesca; Picchio, Vittorio; Angelini, Francesco; Iaccarino, Alessandra; Peruzzi, Mariangela; Biondi‐Zoccai, Giuseppe; Sciarretta, Sebastiano; Falco, Elena De; Chimenti, Isotta; Frati, Giacomo

doi:10.1007/s11886-018-1031-6

Cited by 21 publications

(23 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, we noticed that measurements of regional wall motion parameters by speckle tracking analysis could reveal early changes in matrix remodeling upon CPC injection (17). The anti-fibrotic effects of CPCs seem to be paracrine in nature and seem to be mediated through exosomes, microRNAs, and endoglin (18, 19). The mechanisms of action are not fully understood however, mainly due to a lack of in vivo insights in matrix remodeling and the role of associated CF (20).…”

Section: Introductionmentioning

confidence: 99%

Anti-fibrotic Effects of Cardiac Progenitor Cells in a 3D-Model of Human Cardiac Fibrosis

Gartner

Deddens

Mol

et al. 2019

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

Cardiac fibroblasts play a key role in chronic heart failure. The conversion from cardiac fibroblast to myofibroblast as a result of cardiac injury, will lead to excessive matrix deposition and a perpetuation of pro-fibrotic signaling. Cardiac cell therapy for chronic heart failure may be able to target fibroblast behavior in a paracrine fashion. However, no reliable human fibrotic tissue model exists to evaluate this potential effect of cardiac cell therapy. Using a gelatin methacryloyl hydrogel and human fetal cardiac fibroblasts (hfCF), we created a 3D in vitro model of human cardiac fibrosis. This model was used to study the possibility to modulate cellular fibrotic responses. Our approach demonstrated paracrine inhibitory effects of cardiac progenitor cells (CPC) on both cardiac fibroblast activation and collagen synthesis in vitro and revealed that continuous cross-talk between hfCF and CPC seems to be indispensable for the observed anti-fibrotic effect.

show abstract

Section: Introductionmentioning

confidence: 99%

Anti-fibrotic Effects of Cardiac Progenitor Cells in a 3D-Model of Human Cardiac Fibrosis

Gartner

Deddens

Mol

et al. 2019

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

show abstract

“…At present, three ways of sorting CPCs have been successfully applied for patients with cardiovascular disease: c-kit+ sorted cells, spheroid selection of cardiosphere-derived cells, and Sca1+ sorted cells. Despite different sorting criteria, they possess almost identical transcription profiles, suggesting that they have similar regenerative properties [ 46 ]. Recent evidence suggests that CPCs can replenish adult injured cardiomyocytes and vascular cells through differentiation.…”

Section: Cell Therapy Candidates and Hydrogel Requirementsmentioning

confidence: 99%

Injectable Hydrogels for Improving Cardiac Cell Therapy—In Vivo Evidence and Translational Challenges

Hoeeg

Dolatshahi-Pirouz

Follin

2021

Gels

View full text Add to dashboard Cite

Cell therapy has the potential to regenerate cardiac tissue and treat a variety of cardiac diseases which are currently without effective treatment. This novel approach to treatment has demonstrated clinical efficiency, despite low retention of the cell products in the heart. It has been shown that improving retention often leads to improved functional outcome. A feasible method of improving cell graft retention is administration of injectable hydrogels. Over the last decade, a variety of injectable hydrogels have been investigated preclinically for their potential to improve the effects of cardiac cell therapy. These hydrogels are created with different polymers, properties, and additional functional motifs and differ in their approaches for encapsulating different cell types. Only one combinational therapy has been tested in a clinical randomized controlled trial. In this review, the latest research on the potential of injectable hydrogels for delivery of cell therapy is discussed, together with potential roadblocks for clinical translation and recommendations for future explorations to facilitate future translation.

show abstract

“…are not yet available. Interestingly, even if CPCs show a high differentiation potential in vitro, recent studies suggest that the in vivo direct differentiation following transplantation is not the primary mechanism by which these cells promote cardiac recovery [6] . In fact, a great proportion of CPCs therapeutic effects were shown to be attributable to indirect mechanisms of action able to exert protective and beneficial effects on the endogenous tissue.…”

Section: Adult Cpcsmentioning

confidence: 99%

“…Unfortunately, inflammatory processes following cardiac injury, in addition to the unsuitable ischemic microenvironment and the lack of oxygen, are able to affect this endogenous regenerative process exacerbating tissue damages and promoting adverse remodeling. Accordingly, cardiac cell therapy (CCT) approach, based on isolation, expansion and injection of CPCs, might be a valid alternative to promote and support heart regeneration [5,6] . However, despite the encouraging preclinical and clinical results, these treatments have shown very limited improvement in the long term [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

CPCs and ECM: A Good Mix for Cardiac Regeneration

2018

Med One

View full text Add to dashboard Cite

Despite several improvements in term of diagnosis and prevention, ischemic heart disease still represents one of the principal worldwide causes of death. Cardiac progenitor cells (CPCs) based therapy is considered a valid alternative to heart transplant, but several issues concerning the transplanted cells viability, retention and therapeutic effect need to be solved. Tissue engineering, mixing synthetic or natural polymers with injected cells, could represent the way through which overcomes shortages and set up an effective cardiac regenerative therapy. Nowadays, it is well known that cardiac extracellular matrix (ECM) provides structural and functional integrity, affects cardiac function, development and physiologic repair. In this optic, ECM and ECM-like materials represent functional and biocompatible tools with a great potential to serve as natural or nature-mimicking scaffolds in the field of regenerative medicine. The aim of the present work is to provide an overview on the state of the art and recent advantages on CPCs and scaffold-based therapy for heart regeneration.

show abstract

The Biological Mechanisms of Action of Cardiac Progenitor Cell Therapy

Cited by 21 publications

References 97 publications

Anti-fibrotic Effects of Cardiac Progenitor Cells in a 3D-Model of Human Cardiac Fibrosis

Anti-fibrotic Effects of Cardiac Progenitor Cells in a 3D-Model of Human Cardiac Fibrosis

Injectable Hydrogels for Improving Cardiac Cell Therapy—In Vivo Evidence and Translational Challenges

CPCs and ECM: A Good Mix for Cardiac Regeneration

Contact Info

Product

Resources

About