The biological mechanisms of PCNA and BMP in TMJ adaptive remodeling

Barnouti, Zoe Potres; Owtad, Payam; Shen, Gang; Petocz, Peter; Darendeliler, M. Ali

doi:10.2319/091609-522.1

Cited by 18 publications

(18 citation statements)

References 36 publications

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“…PCNA is an accepted hallmark for cellular proliferation [40,41]. Ki-67, a protein expressed in proliferating cells, may indicate a more robust marker of cell proliferation than PCNA [16].…”

Section: Effect Of -Tocotrienol On the Proliferation Of Mc3t3-e1mentioning

confidence: 99%

Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells

et al. 2018

Journal of Chemistry

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Gamma-tocotrienol, a major component of tocotrienol-rich fraction of palm oil, has been suggested to exhibit bone protective effects in vivo. However, the effects of -tocotrienol on osteoblast cells are still unclear. In this study, the effects of -tocotrienol on the proliferation, differentiation, and mineralization in osteoblastic MC3T3-E1 cells were investigated. Our results showed that -tocotrienol (2-8 mol/L) significantly improved the cell proliferation ( < 0.05), but it did not affect cell cycle progression.-Tocotrienol significantly increased alkaline phosphatase (ALP) activity ( < 0.05), secretion levels of osteocalcin (OC) and osteonectin (ON), and mRNA levels of collagen type I (Col I) of MC3T3-E1 cells. Meanwhile, we found that -tocotrienol is promoted in differentiation MC3T3-E1 cells by upregulation of the expression of Runx2 protein. Moreover, the number of bone nodules increased over 2.5-fold in cells treated with -tocotrienol (2-8 mol/L) for 24 d compared to control group. These results indicated that -tocotrienol at low dose levels, especially 4 mol/L, could markedly enhance the osteoblastic function by increasing the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells. Moreover, our data also indicated that Runx2 protein may be involved in these effects. Further studies are needed to determine the potential of -tocotrienol as an antiosteoporotic agent.

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“…PCNA is an accepted hallmark for cellular proliferation [40,41]. Ki-67, a protein expressed in proliferating cells, may indicate a more robust marker of cell proliferation than PCNA [16].…”

Section: Effect Of -Tocotrienol On the Proliferation Of Mc3t3-e1mentioning

confidence: 99%

Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells

et al. 2018

Journal of Chemistry

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“…21,22 Anterior glenoid fossa remodeling and spontaneous anterior mandibular displacement that occurs after elimination of a functional retrusion also have been cited as contributors to Class II correction. 24,25 The lack of success with functional appliance treatment has been attributed to a lack of patient compliance and the inability to control the amount and direction of mandibular growth. 26 One of the few fixed functional appliances that eliminate this compliance factor is the mandibular anterior repositioning appliance (MARA).…”

Section: Introductionmentioning

confidence: 99%

Treatment effects of the mandibular anterior repositioning appliance in patients with Class II skeletal malocclusions

Pangrazio

Pangrazio-Kulbersh

Berger³

et al. 2012

The Angle Orthodontist

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Objective: To examine the changes produced by the mandibular anterior repositioning appliance (MARA) appliance and compare the treatment effects to an untreated Class II control group. Materials and Methods: Thirty consecutively treated patients were matched with an untreated control group. Lateral cephalograms were taken at T1, 5 months pre-MARA (CVMS 2.7); T2, immediately after MARA removal and prior to placement of full fixed edgewise appliances (CVMS 4.2); and T3, at least 2 years after MARA removal and completion of edgewise treatment (CVMS 5.4). The mean age of the MARA patients was 11.9 years for boys and 10.8 years for girls. Repeated-measures analysis of variance (ANOVA) was used to assess if the samples were morphologically comparable at the outset and to test if there were significant differences between the groups for the various increments of change. Given a significant ANOVA, the source of the difference was explored via Tukey-Kramer tests. Results: Restriction of maxillary growth and no significant mandibular growth were observed with the MARA appliance. The Class II correction was obtained mainly by slight maxillary molar distalization and intrusion, in addition to mesial migration of the lower molars and flaring of the lower incisors. No vertical effect was observed with this appliance. Conclusion: The MARA appliance was effective in the treatment of Class II malocclusions. Restriction of maxillary growth and dentoalveolar changes in the maxillary and mandibular arches were responsible for the correction of the Class II malocclusion. Significant mandibular growth did not contribute to this correction. (Angle Orthod. 2012;82:971-977.)

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“…Since the osteoblast is mainly responsible for bone formation, in the present research, both gain-of-function and loss-of-function approaches were applied to differentiate the conflicting roles of hRnp13 using the human osteoblast-like MG63 cell line, and to investigate the underlying mechanisms of those actions. PCNA is an accepted hallmark for cellular proliferation [3]. In this study, the suppression of hRpn13 increased cellular proliferation and upregulated PCNA in MG63 cells while overexpression reduced cellular proliferation and downregulated PCNA.…”

Section: Discussionmentioning

confidence: 60%

“…RNA isolation and quantitative real-time reverse-transcription PCR RNA isolation and quantitative real-time reversetranscription PCR (qRT-PCR) was performed as described previously [3]. Total RNA was extracted using Trizol reagent from Invitrogen (Carlsbad, CA, USA) according to the manufacturer's instructions.…”

Section: Mtt Colorimetric Assaymentioning

confidence: 99%

“…Accordingly, the altered balance between OPG and RANKL activity has been shown to play pivotal roles in the development of osteoporosis in animals and man [2]. Proliferating cell nuclear antigen (PCNA) is a vital proliferation-related protein [3], and alkaline phosphatase (ALP) activity and osteocalcin (OCN) are important indices for the level of differentiation in osteoblasts [15,17,20].…”

Section: Introductionmentioning

confidence: 99%

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hRpn13, a newly identified component of the 19S particle, regulates proliferation, differentiation, and function in the human osteoblast-like cell line MG63

et al. 2011

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The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. The function of the proteasomal ubiquitin receptor hRpn13, a component of the 26S proteasome, is not completely understood. To investigate the role of hRpn13 in the ubiquitin-proteasome system in osteoblasts, the effects of suppressing and overexpressing the hRpn13 gene on proliferation, differentiation, and function of human osteoblast-like MG63 cells were examined. After knockdown of hRpn13 by small interfering RNA, changes in osteoblast proliferation were evaluated by methylthiazolyl-tetrazolium assay. There was an increase in markers for osteoblast proliferation, specifically alkaline phosphatase activity, and elevated protein levels of osteocalcin, proliferating cell nuclear antigen (PCNA), and ubiquitin. Furthermore, hRpn13 knockdown also resulted in a decrease in the ratio between the gene expressions of RANKL and OPG, key players in the pathogenesis of bone diseases that influence the normal balance between bone formation and resorption. In contrast, overexpression of hRpn13 inhibited the proliferation of MG63 cells, and decreased alkaline phosphatase activity as well as protein levels of osteocalcin, PCNA, and ubiquitin while the ratio of RANKL to OPG expression increased. To confirm the function of hRpn13 in the ubiquitin-proteasome pathway, osteoblast proliferation enhancement and ubiquitin accumulation after hRpn2 knockdown was assessed. The results suggest that overexpression of hRpn13 negatively influences proliferation and osteogenic differentiation in MG63 cells. The evidence implies that hRpn13 modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts. In summary, overexpression of hRpn13 promoted the activity of the ubiquitin-proteasome system.

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The biological mechanisms of PCNA and BMP in TMJ adaptive remodeling

Cited by 18 publications

References 36 publications

Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells

Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells

Treatment effects of the mandibular anterior repositioning appliance in patients with Class II skeletal malocclusions

hRpn13, a newly identified component of the 19S particle, regulates proliferation, differentiation, and function in the human osteoblast-like cell line MG63

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