The biological responses and mechanisms of endothelial cells to magnesium alloy

Abstract

Due to its good biocompatibility and degradability, magnesium alloy (Mg alloy) has shown great promise in cardiovascular stent applications. Rapid stent re-endothelialization is derived from migrated and adhered endothelial cells (ECs), which is an effective way to reduce late thrombosis and inhibit hyperplasia. However, fundamental questions regarding Mg alloy affecting migration and adhesion of ECs are not fully understood. Here, we evaluated the effects of Mg alloy on the ECs proliferation, adhesion and migration. A global gene expression profiling of ECs co-culturing with Mg alloy was conducted, and the adhesion- and migration-related genes were examined. We found that Mg alloy had no adverse effects on ECs viability but significantly affected ECs migration and adhesion. Co-cultured with Mg alloy extract, ECs showed contractive adhesion morphology and decreased motility, which was supported by the down-regulation of adhesion-related genes (Paxillin and Vinculin) and migration-related genes (RAC 1, Rho A and CDC 42). Accordingly, the re-endothelialization of Mg alloy stent was inhibited in vivo. Our results may provide new inspiration for improving the broad application of Mg alloy stents.

UV‐Triggered Hydrogel Coating of the Double Network Polyelectrolytes for Enhanced Endothelialization

“…[ 23 ] Rac Family Small GTPase 1 (RAC 1) and Ras Homolog Family Member A (Rho A) are essential Rho GTPases that regulate cell motility. [ 24 ] The higher expression of RAC 1 and Rho A further confirmed that ECs had a high migration ability on the A45R1 coating. The rapid migration of ECs to the material surface accelerates endothelialization.…”

Integrating Anion−π+ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway

“…This was further supported by the decreased expression of Ki67, which serves as a cell proliferation indicator (Figure S46). 33 Meanwhile, the cytocidal ability of DBQ-2DPA NPs, DBQ-2TPA NPs, and DBQ-4TPA NPs was also assessed through the CCK-8 assay. As anticipated, DBQ-2DPA-4TPA NPs revealed superior phototoxicity toward A375 cells compared to the other AIE NPs, which can be attributed to its higher ROS generation ability (Figure S47a−c).…”

Yes-associated protein contributes to magnesium alloy-derivedinflammation in endothelial cells

“…Western blotting was conducted as described previously [ 25 ]. All experiments were quantified by Image lab (Bio-Rad) and normalized to internal control.…”