The biological sink of atmospheric H2 is more sensitive to spatial variation of microbial diversity than N2O and CO2 emissions in a winter cover crop field trial

Baril, Xavier; Durand, Audrey‐Anne; Srei, Narin; Lamothe, Steve; Provost, Caroline; Martineau, Christine; Dunfield, Kari E.; Constant, Philippe

doi:10.1016/j.scitotenv.2022.153420

Cited by 3 publications

(3 citation statements)

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“…The low water content in soil and the promotion of gaseous exchanges by a large headspace-to-soil volume ratio of 15.7 and a soil layer thickness of 0.5 cm, promoting aerobic conditions. H 2 oxidation rates were measured weekly during the incubation period with a gas chromatographic assay conducted with an initial H 2 concentration of 482 ± 142 ppbv (Baril et al 2022 ). H 2 oxidation activity measurements were conducted by integrating the linear decay of H 2 concentrations in the static headspace of soil microcosms from 5 to 6 observations recorded within less than 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…The abundance of bacterial 16S rRNA gene, fungal ITS, hhyL gene encoding the large subunit of group 5/1 h NiFehydrogenase were determined by droplet digital PCR (ddPCR) as described in Baril et al ( 2022 ). Briefly, three runs of ddPCR were performed with the ddPCR Gene Expression EvaGreen® Assays (Bio-Rad, Hercules, USA).…”

Section: Methodsmentioning

confidence: 99%

“…At the laboratory scale, Khdhiri et al ( 2015 ) proposed a linear model parameterized with soil total carbon content explained the variation of H 2 uptake rate measured in contrasting soil samples. Other variables including HA-HOB relative abundance, microbial community composition, alpha diversity and pH were correlated with H 2 oxidation rates (Gödde et al 2000 , Khdhiri et al 2015 , Saavedra-Lavoie et al 2020 , Baril et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Carbon amendments in soil microcosms induce uneven response on H2 oxidation activity and microbial community composition

Baril,

Constant

2023

FEMS Microbiology Ecology

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High-affinity H2-oxidizing bacteria (HA-HOB) thriving in soil are responsible for the most important sink of atmospheric H2. Their activity increases with soil organic carbon content, but the incidence of different carbohydrate fractions on the process has received little attention. Here we tested the hypothesis that carbon amendments impact HA-HOB activity and diversity differentially depending on their recalcitrance and their concentration. Carbon sources (sucrose, starch, cellulose) and application doses (0, 0.1, 1, 3, 5% Ceq soildw−1) were manipulated in soil microcosms. Only 0.1% Ceq soildw−1 cellulose treatment stimulated the HA-HOB activity. Sucrose amendments induced the most significant changes, with an abatement of 50% activity at 1% Ceq soildw−1. This was accompanied with a loss of bacterial and fungal alpha diversity and a reduction of high-affinity group 1 h/5 [NiFe]-hydrogenase gene (hhyL) abundance. A quantitative classification framework was elaborated to assign carbon preference traits to 16S rRNA gene, ITS and hhyL genotypes. The response was uneven at the taxonomic level, making carbon preference a difficult trait to predict. Overall, the results suggest that HA-HOB activity is more susceptible to be stimulated by low doses of recalcitrant carbon, while labile carbon-rich environment is an unfavorable niche for HA-HOB, inducing catabolic repression of hydrogenase.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%