The biology and in vitro propagation of the ornamental aquatic plant, Aponogeton ulvaceus

Abstract

Aponogeton ulvaceus Baker (Aponogetonaceae) is a commercially important ornamental aquatic plant species with traditional medicinal uses. Due to the low survival rate of seedlings, propagation by conventional means has been met with many difficulties. In this study, botanical aspects of A. ulvaceus were examined with regards to the morphology, anatomy and physiology of the plant and an efficient protocol for its in vitro propagation using immature tuber explants has been established. The existence of glandular trichomes on the leaves was discovered and the occurrence of circumnutation in A. ulvaceus has been demonstrated. Immature tuber segments with meristems were cultured on MS medium supplemented with various combinations (0, 1, 2, and 3 mg/L) of BAP and NAA for callus induction. The highest percentage of callus production (100 %) was obtained in two different treatments: 1 mg/L BAP and 3 mg/L NAA, and 2 mg/L BAP and 3 mg/L NAA. For shoot and root organogenesis, the combination of 1 mg/L BAP and 1 mg/L NAA was shown to be significant for A. ulvaceus regeneration when compared to control, which yields a mean shoot and root number of 22.50 and 29.50 respectively. The current protocol is the first reported successful establishment of in vitro clonal propagation of A. ulvaceus.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-3041-4) contains supplementary material, which is available to authorized users.

Rapid Clonal Multiplication Through In Vitro Axillary Shoot Proliferation of Lobelia cardinalis L., an Ornamental Aquatic Plant

“…The present finding further indicated that the inclusion of an auxin (IAA or NAA) along with the optimal cytokinin (BAP) concentration improved the multiplication rate. Other research groups also found that the combination of BAP and NAA proved best for the micropropagation of some other ornamental aquatic plants, such as Anubias Heterophylla [16], Aponogeton ulvaceus Baker [32], Echinodorus 'Indian Red' [33], and Lindernia antipoda (L.) Alston [34], as well as for the medicinal herb Artemisia abrotanum L. [35]. However, these findings disagree with those of Sheeja et al [36], who reported that BAP alone proved better than the combination of BAP and NAA for the micropropagation of an ornamental aquatic plant Anubias barteri Var.…”