The biology of breast cancer at the cellular level

Smith, Helene S.; Wolman, Sandra R.; Hackett, Adeline J.

doi:10.1016/0304-419x(84)90009-x

Cited by 31 publications

(28 citation statements)

References 81 publications

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“…A major deterrent to research has been the lack of suitable cell culture systems in which primary tumor cells could be grown and compared with the normal mammary epithelial cells of origin and in which the stepwise process of mammary tumorigenesis could be investigated (1). The recent development of a medium, DFCI-1, in our laboratory should alleviate this problem (2); newly isolated cell lines from primary tumors are now being studied (ref.…”

mentioning

confidence: 99%

Human papilloma virus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements.

Band

Zajchowski

Kulesa

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

267

225

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Human papilloma virus (HPV) types 16 and 18 are most commonly associated with cervical carcinoma in patients and induce immortalization of human keratinocytes in culture. HPV has not been associated with breast cancer. This report describes the immortalization of normal human mammary epithelial cells (76N) by plasmid pHPV18 or pHPV16, each containing the linearized viral genome. Transfectants were grown continuously for more than 60 passages, whereas 76N cells senesce after 18-20 passages. The transfectants also differ from 76N cells in cloning in a completely defined medium called D2 and growing in a minimally supplemented dermed medium (D3) containing epidermal growth factor. All transfectants tested contain integrated HPV DNA, express HPV RNA, and produce HPV E7 protein. HPV transfectants do not form tumors in a nude mouse assay. It is concluded that products of the HPV genome induce immortalization of human breast epithelial cells and reduce their growth factor requirements. This result raises the possibility that HPV might be involved in breast cancer. Furthermore, other tissue-specific primary epithelial cells that are presently difficult to grow and investigate may also be immortalized by HPV.Breast cancer is a very frequent lethal malignancy of women in North America and western Europe, but the underlying molecular genetics and pathobiology are poorly understood. A major deterrent to research has been the lack of suitable cell culture systems in which primary tumor cells could be grown and compared with the normal mammary epithelial cells of origin and in which the stepwise process of mammary tumorigenesis could be investigated (1). The recent development of a medium, DFCI-1, in our laboratory should alleviate this problem (2); newly isolated cell lines from primary tumors are now being studied (ref.

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mentioning

confidence: 99%

Human papilloma virus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements.

Band

Zajchowski

Kulesa

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

267

225

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“…The MCF-7 has contributed to biological analyses of breast cancer cells, i.e., studies on estrogen-receptor, oncogene expression, and tumoricidal effects of chemo-endocrine agents (Butler et al 1981;Sutherland et al 1983 ; Osborne et al 1985 ; Gottardis et al 1989; Sommers et al 1990). Breast cancers differ, however, as far as their biological behavior and tumor cell heterogeneity are concerned (Smith et al 1984 ; Chu et al 1985;Band et al 1990; Petersen et al 1990). …”

mentioning

confidence: 99%

Establishment of An Estrogen Receptor-Positive Cell Line (HMA-1) Derived from Human Breast Carcinoma.

Matano

Ohuchi

Higuchi

et al. 1991

Tohoku J. Exp. Med.

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We have established a novel human breast carcinoma cell line, HMA-1, derived from ascites of a female breast cancer patient. HMA-1 was shown to be an epithelial cell line with intracytoplasmic duct-like vacuoles, microvilli, desmosomes and tonofibrils in accordance with human breast cancer. The cell line demonstrated a good cell growth ability in monolayer fashion with a doubling time of 46 hr. Based on a whole cell binding assay the cell line contained estrogen receptor (1.45>< 10-4 sites/cell). Tamoxifen, an anti-estrogen agent induced a dose-dependent decrease in the cell growth rate, but estradiol stimulated the cell growth. HMA-1 could be transplanted subcutaneously into BALB/c nude mice, and was able to cause tumors approximately two months after heteroinoculation. These results indicate that HMA-1 cell line may serve as a new human breast carcinoma cell line which could be utilized in the breast cancer research. breast neoplasm ; human cell line ; estrogen receptor Breast carcinoma remains the leading cause of cancer mortality among women in western countries and its incidence is rising worldwide including Japan. Human breast carcinoma cell lines are needed for multidisciplinary research in breast cancer. There are a few well-characterized cell lines, derived from human breast carcinomas. The first report attempting to culture breast carcinoma cells appeared in 1937 (Cameron and Chambers), but there were no other reports until 1958 when Lasfargues and Ozzello established the first successful long-term culture of human breast carcinoma cells . Since then various kinds of human breast cancer cell lines have been reported (Soule et al. 1973 ;Trempe and Fogh 1973;Cailleau et al. 1974; Breast Cancer Task Force Cell Culture Bank,

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“…A major reason has been the difficulty in growing either normal or tumor-derived human mammary epithelial cells in culture (1)(2)(3). Even the establishment of tumor cell lines has been an infrequent accomplishment (4)(5)(6).…”

mentioning

confidence: 99%

Distinctive traits of normal and tumor-derived human mammary epithelial cells expressed in a medium that supports long-term growth of both cell types.

Band

Sager

1989

Proc. Natl. Acad. Sci. U.S.A.

232

169

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A medium is described that supports longterm growth in culture of human primary mammary tumor cells, of normal epithelial cells from mammoplasty, and of mammary tumor cell lines. Tumor cells are shown to be distinguishable from normal mammary epithelial cells by morphology, by growth requirements, and by two markers: preferential expression of the HMFG-2 epitope on tumor cells and preferential retention in tumor cell mitochondria of the lipophilic fluorescent dye rhodamine 123. Differential fluorescence of HMFG-2 fluorescein-conjugated antibodies can be used as a basis for separation of normal and tumor cells in a fluorescence-activated cell sorter, as can differential retention of rhodamine 123.

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The biology of breast cancer at the cellular level

Cited by 31 publications

References 81 publications

Human papilloma virus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements.

Human papilloma virus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements.

Establishment of An Estrogen Receptor-Positive Cell Line (HMA-1) Derived from Human Breast Carcinoma.

Distinctive traits of normal and tumor-derived human mammary epithelial cells expressed in a medium that supports long-term growth of both cell types.

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