The biology of reactive intermediates in systemic lupus erythematosus

Oates, Jim C.

doi:10.3109/08916930903374683

Cited by 46 publications

(61 citation statements)

References 60 publications

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“…Interestingly, despite the reduction in NO production, iNOS protein levels were not reduced in the 17-DMAG-treated immune-stimulated cells. We would expect iNOS expression to be reduced with suppression of NF-jB translocation [45,61]. While we did observe reduced NF-jB translocation with 17-DMAG treatment in immune-stimulated cells, we did not find a reduction in iNOS expression, suggesting that inhibiting NF-jB translocation may not be sufficient to down-regulate iNOS expression.…”

Section: Discussioncontrasting

confidence: 54%

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HSP90 inhibition by 17-DMAG reduces inflammation in J774 macrophages through suppression of Akt and nuclear factor-κB pathways

et al. 2012

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Section: Discussioncontrasting

confidence: 54%

“…NO production can be induced by immune stimulation [61]. Recently it was shown that HSP70 can function as an inhibitor of iNOS [62].…”

Section: Discussionmentioning

confidence: 99%

HSP90 inhibition by 17-DMAG reduces inflammation in J774 macrophages through suppression of Akt and nuclear factor-κB pathways

et al. 2012

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“…As occurs with other autoimmune diseases, inflammation and oxidative stress are frequent in the course of SLE (3,4). According to this, some authors have reported higher levels of the biomarker of inflammation, C-reactive protein (CRP), in these chronic patients compared to controls (4)(5)(6).…”

Section: Introductionmentioning

confidence: 84%

Microbiota and oxidant-antioxidant balance in systemic lupus erythematosus

et al. 2017

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ResumenBackground: Systemic lupus erythematosus (SLE) is a chronic infl ammatory disease of autoimmune nature, in which oxidative stress is implicated. Aim: Compare the concentrations of dietary and blood antioxidants, as well as gut microbiota, with serum malondialdehyde (MDA) and C reactive protein (CRP) in 21 subjects suffering from non-active systemic lupus erythematosus (SLE) and 21 age and gender-matched controls. Methods: General biochemical parameters and CRP were determined by enzymatic methods: copper, zinc and selenium by inductively coupled plasma mass spectrometry (ICP-MS), MDA and total antioxidant capacity (TAC) by spectrophotometric methods, gut microbiota by metagenomic analyses and dietary intake by means of food frequency questionnaire. Results: No signifi cant differences were found in diet between lupus patients and the control group, with the exception of trans fatty acids intake, which was higher in patients. In addition, higher concentration of serum copper and lower of zinc in SLE were found. Serum copper was positively associated with CRP and also, this protein with the proportion of Lentisphaerae, Proteobacteria and Verrucomicrobia in feces. Moreover, MDA levels displayed inverse correlations with the Cyanobacteria and Firmicutes groups, while Actinobacteria showed a positive association. The lupus subjects with presence of anti-SSA/Ro were related to higher levels of serum zinc. Conclusion: These results could be useful in the future to go deeper into the understanding of this complex disease. AbstractIntroducción: el lupus eritematoso sistémico es una enfermedad infl amatoria crónica en la que está implicado el estrés oxidativo. Objetivo: evaluar la concentración de antioxidantes de la dieta y sanguíneos, así como de la microbiota sobre las concentraciones de malondialdehído y proteína C reactiva en 21 pacientes de lupus y 21 controles pareados por edad y sexo. Métodos: los parámetros bioquímicos de rutina y proteína C reactiva se determinaron a través de métodos enzimáticos: cobre, zinc y selenio por espectrometría de masas, malondialdehído y capacidad antioxidante total por métodos espectrofotométricos, la microbiota fecal por técnicas metagenómicas y la dieta a través de cuestionarios de frecuencia de consumo. Resultados: no se han observado diferencias en la dieta en los pacientes con lupus respecto al grupo control, excepto en la ingesta de ácidos grasos trans, siendo mayor en el grupo de lupus. En estas pacientes se observaron mayores niveles circulantes de cobre y menores de zinc. La concentración de cobre en suero se relacionó directamente con los niveles de proteína C reactiva y esta proteína, a su vez, con la proporción de Lentisphaerae, Proteobacteria y Verrucomicrobia en heces. Además, mientras que los niveles de malondialdehído se asociaban inversamente con la proporción de Cyanobacteria y Firmicutes, con Actinobacteria se encontró una correlación positiva. La presencia de anti-SSA/Ro en lúpicas se relaciona con mayores concentraciones de zinc. Conclusió...

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“…Al-Griw et al Open Veterinary Journal, (2016), Vol. 6(3): 150-157 reported in various diseases (Khan et al, 2001;Morgan et al, 2005;Oates, 2010;Wang et al, 2013). Proteins perform crucial functions within living cells, but even a relatively minor structural modification of proteins often leads to a marked change (generally lowering) in their functions (Orengo et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Cellular and molecular etiology of hepatocyte injury in a murine model of environmentally induced liver abnormality

Al-Griw¹,

Alghazeer²,

Al-Azreg³

et al. 2016

Open Vet J.

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Exposures to a wide variety of environmental substances are negatively associated with many biological cell systems both in humans and rodents. Trichloroethane (TCE), a ubiquitous environmental toxicant, is used in large quantities as a dissolvent, metal degreaser, chemical intermediate, and component of consumer products. This increases the likelihood of human exposure to these compounds through dermal, inhalation and oral routes. The present in vivo study was aimed to investigate the possible cellular and molecular etiology of liver abnormality induced by early exposure to TCE using a murine model. The results showed a significant increase in liver weight. Histopathological examination revealed a TCE-induced hepatotoxicity which appeared as heavily congested central vein and blood sinusoids as well as leukocytic infiltration. Mitotic figures and apoptotic changes such as chromatin condensation and nuclear fragments were also identified. Cell death analysis demonstrates hepatocellular apoptosis was evident in the treated mice compared to control. TCE was also found to induce oxidative stress as indicated by an increase in the levels of lipid peroxidation, an oxidative stress marker. There was also a significant decrease in the DNA content of the hepatocytes of the treated groups compared to control. Agarose gel electrophoresis also provided further biochemical evidence of apoptosis by showing internucleosomal DNA fragmentation in the liver cells, indicating oxidative stress as the cause of DNA damage. These results suggest the need for a complete risk assessment of any new chemical prior to its arrival into the consumer market.

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The biology of reactive intermediates in systemic lupus erythematosus

Cited by 46 publications

References 60 publications

HSP90 inhibition by 17-DMAG reduces inflammation in J774 macrophages through suppression of Akt and nuclear factor-κB pathways

HSP90 inhibition by 17-DMAG reduces inflammation in J774 macrophages through suppression of Akt and nuclear factor-κB pathways

Microbiota and oxidant-antioxidant balance in systemic lupus erythematosus

Cellular and molecular etiology of hepatocyte injury in a murine model of environmentally induced liver abnormality

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