The biomedical effects of laser application

Mester, E; Mester, Á.

doi:10.1002/lsm.1900050105

Cited by 712 publications

(455 citation statements)

References 7 publications

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“…Such data corroborate the results from other authors who have used LILT during 12 days of tissue repair processes and verified responses from the fibroblastic cells and collagen molecules to the treatment 7,8,[19][20][21][22][23][24][25][26][27][28] . The types of activation ways proposed for the laser, take into account its action over the chromophores situated at the mitochondrias and cell membranes.…”

Section: Discussionsupporting

confidence: 90%

Influência de diferentes comprimentos de onda da laserterapia de baixa intensidade na regeneração tendínea do rato após tenotomia

Erb

Rodrigues

Taciro

et al. 2007

Rev. bras. fisioter.

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Objective: The aim of this study was to compare the effect of the low level laser therapy in the organization of the collagen fibers with 3 J/cm² of dose in two wavelengths (670nm and 904nm) and in the combination of both on the tissue repair of the calcaneus tendon. Methods: We used 37 female Wistar rats divided in 5 groups which had been submitted to the total tenotomy. Group GA (n= 8) was submitted to the laser irradiation in λ= 904nm, GaAS, pulsated emission, peak power 15W and dose of 3J/ cm², group GB (n= 8) was submitted to laser irradiation λ= 670nm AlGaInP diode, continuous emission, power 30 mW and dose 3J/cm², group GAB (n= 8) was submitted to association of both of lasers and group GCL (n= 7) which the calcaneum tendon was submitted to placebo treatment. The group GP (n= 6) was a standard group without lesion and treatment. The animals received 12 sessions of lasertherapy and submitted to euthanasia on 14 th day after surgery. The tendons were submitted to histological procedure and were analyzed to evaluate the optic retardation (in nm) for the birefringence measurement. Results: The statistic analysis showed that the irradiated groups have higher values of optic retardation statistically significant than placebo group (p= 0,00001). It indicates that all the treated groups show better organization of collagen fibers in the longitudinal axis of the tendon, evidencing better quality in the tissue repair. The group GAB was that shown best results (p= 0,00001) when compared to another groups.

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Section: Discussionsupporting

confidence: 90%

Influência de diferentes comprimentos de onda da laserterapia de baixa intensidade na regeneração tendínea do rato após tenotomia

Erb

Rodrigues

Taciro

et al. 2007

Rev. bras. fisioter.

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“…Mester observed that in many cases the skin incisions made to implant recalcitrant cells appeared to heal faster in treated animals compared to incisions of control animals that were not treated with light [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium‐neon laser irradiation

2006

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Background: In medicine, lasers have been used predominantly for applications, which are broadly termed low level laser therapy (LLLT), phototherapy or photobiomodulation. This study aimed to establish cellular responses to Helium-Neon (632.8 nm) laser irradiation using different laser fluences (0.5, 2.5, 5, 10, and 16 J/cm 2 ) with a single exposure on 2 consecutive days on normal and wounded human skin fibroblasts. Materials and Methods: Changes in normal and wounded fibroblast cell morphology were evaluated by light microscopy. Changes following laser irradiation were evaluated by assessing the mitochondrial activity using adenosine triphosphate (ATP) luminescence, cell proliferation using neutral red and an alkaline phosphatase (ALP) activity assay, membrane integrity using lactate dehydrogenase (LDH), and percentage cytotoxicity and DNA damage using the Comet assay. Results: Morphologically, wounded cells exposed to 5 J/ cm 2 migrate rapidly across the wound margin indicating a stimulatory or positive influence of phototherapy. A dose of 5 J/cm 2 has a stimulatory influence on wounded fibroblasts with an increase in cell proliferation and cell viability without adversely increasing the amount of cellular and molecular damage. Higher doses (10 and 16 J/cm 2 ) were characterized by a decrease in cell viability and cell proliferation with a significant amount of damage to the cell membrane and DNA. Conclusions: Results show that 5 J/cm 2 stimulates mitochondrial activity, which leads to normalization of cell function and ultimately stimulates cell proliferation and migration of wounded fibroblasts to accelerate wound closure. Laser irradiation can modify cellular processes in a dose or fluence (J/cm 2 ) dependent manner. Lasers Surg.

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“…Since the report of Mester et al [1]. on low-level laser irradiation of mouse skin to investigate wound healing, many researchers have investigated the influence of laser irradiation on wound healing [1][2][3][4][5], improvement of microcirculation [6], nerve activation [7], treatment of chronic pain and anti-inflammation [8]. The influence of low-level laser irradiation on activation of salivary gland cells has been also investigated [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…The influence of low-level laser irradiation on activation of salivary gland cells has been also investigated [9][10][11][12][13]. Previous investigations showed degenerative and inflammatory changes after helium/neon (He-Ne) low-level laser 4 irradiation, histopathologically [9,10]. On the other hand, another study found no detectable degenerative changes in rat submandibular gland cells after gallium-arsenide (Ga-As) low-level laser irradiation [13].…”

Section: Introductionmentioning

confidence: 99%

Low-level (gallium-aluminum-arsenide) laser irradiation of Par-C10 cells and acinar cells of rat parotid gland

et al. 2008

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We investigated cell response, including cell proliferation and expression of heat stress protein to clarify the influence of low-level (Ga-Al-As diode) laser irradiation on rat parotid gland acinar cell-derived Par-C10 cells. Furthermore, we also investigated amylase release and cell death to irradiation in rat parotid gland acinar cells. Number of Par-C10 cells in the laser-irradiated groups was higher than that in the non-irradiated group at days 5 and 7, although the difference was statistically significant (p<0.01).Higher expression of HSP25 and bcl-2 was seen at days 1 and 3 in the irradiated group.Amylase release assay showed no significant difference between irradiated group and non-irradiated group statistically. Trypan blue exclusion assay revealed that there was no difference in the ratio of dead to live cells between the irradiated-and non-irradiated groups. These results suggest that low-level laser irradiation promotes cell proliferation and expression of anti-apoptosis proteins in Par-C10 cells, but it does not significantly affect amylase secretion and does not induce rapid cell death in isolated rat parotid gland acinar cells.3

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The biomedical effects of laser application

Cited by 712 publications

References 7 publications

Influência de diferentes comprimentos de onda da laserterapia de baixa intensidade na regeneração tendínea do rato após tenotomia

Influência de diferentes comprimentos de onda da laserterapia de baixa intensidade na regeneração tendínea do rato após tenotomia

The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium‐neon laser irradiation

Low-level (gallium-aluminum-arsenide) laser irradiation of Par-C10 cells and acinar cells of rat parotid gland

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