The biomolecular corona is retained during nanoparticle uptake and protects the cells from the damage induced by cationic nanoparticles until degraded in the lysosomes

Wang, Fengjuan; Lu, Yu; Monopoli, Marco P.; Sandin, Peter; Mahon, Eugene; Salvati, Anna; Dawson, Kenneth A.

doi:10.1016/j.nano.2013.04.010

Cited by 374 publications

(397 citation statements)

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“…Nanoparticles can easily cross the cell 1 3 membranes and interact with intracellular metabolism [13]. ROS formation is one of the mechanisms for nanoparticle toxicity [14,15]. Interaction of nanoparticles with cells induces pro-oxidant effects leading to ROS generation, mitochondrial respiration and NADPH-dependent enzyme systems [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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Section: Introductionmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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“…Using acidotropic probes, such as LysoTracker, an eventual increase in lysosomal staining would be indicative of lysosomal swelling, while formation of a second peak at lower intensity could be sign of compromised lysosomal membrane integrity. 34 However, we found that increasing the concentration of 100 nm PS COOH NPs to 300 µg/ml over 48 h exposure did not greatly alter the lysosomal acidic compartments of the hCMEC/D3 monolayer, as shown in Figure 3. This may be explained by the relatively low uptake levels in the barrier, in comparison to single cells, or may suggest that the hCMEC/D3 barrier has a very high capacity to accommodate PS COOH NPs, and even at very high NP loading the lysosome size and integrity was not perturbed greatly.…”

Section: Resultsmentioning

confidence: 74%

Paracrine signalling of inflammatory cytokines from an in vitro blood brain barrier model upon exposure to polymeric nanoparticles

Raghnaill

Bramini

Dong

et al. 2014

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Nanoparticle properties, such as small size relative to large highly modifiable surface area, offer great promise for neuro-therapeutics and nanodiagnostics. A fundamental understanding and control of how nanoparticles interact with the blood-brain barrier (BBB) could enable major developments in nanomedical treatment of previously intractable neurological disorders, and help ensure that nanoparticles not intended to reach the brain do not cause adverse effects. Nanosafety is of utmost importance to this field. However, a distinct lack of knowledge exists regarding nanoparticle accumulation within the BBB and the biological effects this may induce on neighbouring cells of the Central Nervous System (CNS), particularly in the long-term. This study focussed on the exposure of an in vitro BBB model to model carboxylated polystyrene nanoparticles (PS COOH NPs), as these nanoparticles are well characterised for in vitro experimentation and have been reported as non-toxic in many biological settings. TEM imaging showed accumulation but not degradation of 100 nm PS COOH NPs within the lysosomes of the in vitro BBB over time. Cytokine secretion analysis from the in vitro BBB post 24 h 100 nm PS COOH NP exposure showed a low level of proinflammatory RANTES protein secretion compared to control. In contrast, 24 h exposure of the in vitro BBB endothelium to 100 nm PS COOH NPs in the presence of underlying astrocytes caused a significant increase in pro-survival signalling. In conclusion, the tantalising possibilities of nanomedicine must be balanced by cautious studies into the possible long-term toxicity caused by accumulation of known 'toxic' and 'non-toxic' nanoparticles, as general toxicity assays may be disguising significant signalling regulation during long-term accumulation.

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“…This, in turn, results in damage to the mitochondria and activation of caspases 3 and 7, with consequent cleavage of PARP-1, ultimately resulting in the apoptotic death of the cells [112]. Ongoing work has shown that the kinetics of the lysosome membrane damage can be correlated with the digestion of the nanoparticle protein corona in the lysosomes which allows the underlying amine groups on the nanoparticles to be re-exposed [113]. Smaller, fully aminated, dendritic polymer nanoparticles have been seen to cause endosomolysis prior to transfer to lysosomes, however, and have been seen to be later localized in the mitochondria.…”

Section: The Signalling Concept: Interaction Of Nanoparticles With Mamentioning

confidence: 98%

Section: The Kinetics Concept: Timescales Of Interaction Of Nanopartimentioning

confidence: 99%

“…In particular, there is emerging evidence that the bio-nano interface can be degraded upon localisation of the nanoparticles in endosomes or lysosomes [113], and indeed even that some nanoparticles themselves, including carbon nanotubes, may degrade in situ in cells or in vivo [121]. Using fluorescently labelled proteins in the nanoparticle corona, it has been possible to track nanoparticle localisation and corona digestion and to correlate this with the toxicity impacts observed [113]. Using amine-modified polystyrene nanoparticles, a detailed event sequence has been tracked showing how nanoparticle location and biological responses are connected -nanoparticles are localised in the lysosomes undergo acid-degradation of the corona, leading to re-expression of the positive charge on the nanoparticles, which were previously masked by the presence of the protein corona, and consequently disruption of the lysosomal wall, leading to a complex set of signalling responses [113].…”

Section: The Kinetics Concept: Timescales Of Interaction Of Nanopartimentioning

confidence: 99%

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The bio-nano-interface in predicting nanoparticle fate and behaviour in living organisms: towards grouping and categorising nanomaterials and ensuring nanosafety by design

et al. 2013

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Abstract:In biological media, nanoparticles acquire a coating of biomolecules (proteins, lipids, polysaccharides) from their surroundings, which reduces their surface energy and confers a biological identity to the particles. This adsorbed layer is the interface between the nanomaterial and living systems and therefore plays a significant role in determining the fate and behaviour of the nanoparticles. This review summarises the state of the art in terms of understanding the bio-nano interface and provides direction for potential future research and recommendations for future priorities and strategies to support the safe implementation of nanotechnologies. The central premise is that nanomaterials must be studied as biological entities under the appropriate exposure conditions and that this should be implemented in study design and reporting for nanosafety assessment. The implications of the bio-nano interface for nanomaterials fate and behaviour are described in light of four interlinked perspectives: the Coating concept; the Translocation concept; the Signalling concept, and the Kinetics concept. A key conclusion is that nanoparticles cannot be viewed as non-interacting species, but rather must be thought of, and studied as, biological entities, where their interaction with the environment is mediated by the proteins and other biomolecules that adsorb to them, and the key parameter to characterise then becomes the nature, composition and evolution of the bionano interface.

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The biomolecular corona is retained during nanoparticle uptake and protects the cells from the damage induced by cationic nanoparticles until degraded in the lysosomes

Cited by 374 publications

References 42 publications

In vitro and in vivo toxicity assessment of nanoparticles

In vitro and in vivo toxicity assessment of nanoparticles

Paracrine signalling of inflammatory cytokines from an in vitro blood brain barrier model upon exposure to polymeric nanoparticles

The bio-nano-interface in predicting nanoparticle fate and behaviour in living organisms: towards grouping and categorising nanomaterials and ensuring nanosafety by design

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