The biosynthesis of 5-hydroxymethyldeoxyuridylic acid in bacteriophage-infected Bacillus subtilis

Roscoe, D.H.; Tucker, R. G.

doi:10.1016/0042-6822(66)90205-4

Cited by 66 publications

(23 citation statements)

References 33 publications

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“…Parmi les bacteriophages de Bacillus subtilis, certains ont la particularit6 dc contenir dam leur DNA une base inhabituelle remplapant entierement la thymine: c'est le cas par exemple du phage transducteur PBS 1 (Takahashi [l]), dont le DNA contient de l'uracile, ou de SP8, qui fait partie du groupe 1 de phages de B. subtilis (Romig et (Roscoe et Tucker [5a,5b]), SPOl (Okubo [6]), 2C (Pene et Marmur [7]), SP5 (Aposhian [S]); leurs DNA ont en commun certaines caracteristiques, outre la presence de I'hydroxy-methyluracile : basse temperature de fusion, densit6 apparente Blevee dans un gradient de CsC1, diffbrence naturelle de densite entre les deux chaines compl6-mentaires de la moMcule, qui se traduit par une distribution ((bimodale)) du DNA denature dans un gradient de CsC1.…”

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“…D'autres phages proches, partageant la m&me particularit6 ont BtB par la suite isoles sBpar&ment, et BtudiBs dans les laboratoires diffhrents : SP82 (Green [4]), #e (Roscoe et Tucker [5a,5b]), SPOl (Okubo [6]), 2C (Pene et Marmur [7]), SP5 (Aposhian [S]); leurs DNA ont en commun certaines caracteristiques, outre la presence de I'hydroxy-methyluracile : basse temperature de fusion, densit6 apparente Blevee dans un gradient de CsC1, diffbrence naturelle de densite entre les deux chaines compl6mentaires de la moMcule, qui se traduit par une distribution ((bimodale)) du DNA denature dans un gradient de CsC1.…”

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Étude comparative des DNA de phages 2C, SP 8*, SP 82, φe, SP 01 et SP 50

Truffaut

Révet

Soulie

1970

European Journal of Biochemistry

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The following Bacillus subtilis phages: SP8*, SP82, @e, SPO1,2C and SP50, were studied and compared for morphological characteristics as well as for physico-chemical properties of their DNA. All the determinations were efiected under identical conditions for the six different phages. The aspect and variety of plaques formed by them with susceptible strain B. subtilis 168-2 (as indicator host), on Difco nutrient agar plates, are slightly different from phage to phage, but do not allow differenciation.As shown by electron microscopy measurements, the five phages SP8*, SP82, SPO1, @e, and 2C are morphologically identical and easily distinguishable from SP50, but not from each other.As for the physico-chemical properties of their DNA, the first five phages are clearly different from SP50 DNA which was taken as a reference: (a) their analytical base composition is consistently the same, all of them containing hydroxymethyluracil instead of thymine in their DNA, and the analyzed percentage guanine plus cytosine is 390/,, as compared to 41.6O/, for SP50 DNA which has no unusual bases; (b) sedimentation coefficients for all the DNA (including SP50 DNA) are about 51 S, a t a concentration of 20 p.g/ml ( $ 2 0 ,~) ; (c) the melting point in 0.15 M NaCI, 0.015M Na citrate is 77.5", without any significant variation among the first group (as compared to 87.3", for SPBO, which is approximately the same as the host Bacillus subtilis 168-2); (d) in a cesium chloride gradient, the buoyant densities of the native DNA of the first group are all 1.742 g/cm3. Since the complementary strands differ in average base composition, two bands of buoyant density 1.752 and 1.762 g/cm3 are obtained following denaturation; (e) all denatured DNA become associated with polyriboguanylic acid [poly(G)], which is preferentially bound to the heavy component, although a slight binding to the light component also occured.Preparative centrifugation in CsCl density gradients in the presence of poly G results in good separation of the two components; (f) the extent of homologous base sequence among the six phages has been determined by DNA/DNA hybridization : hybridization of 2C DNA, SPOl DNA and SP82 DNA with 2C DNA is experimentally identical and assumed to represent 100°/, homology. However for SP8 DNA/2C DNA and @e DNA/2C DNA, the percentage is lower, amounting t o 92O/, and 83O/,, respectively. With SP50 and B. subtilis DNA it is only 7O/,.On the whole, there are no apparent properties, peculiar to one of the 2C, SP8*, SP82, @e, and SPOl phages, allowing its differentiation from the others, whereas SP50 is morphologically distinct, particularly by the length of its tail. It also differs by diverse physical and biological properties. All the common characteristics possessed by the first group of phages, particularly the high extent of DNA/DNA hybridization among them, and the fact that the phages and their DNA are serologically related, demonstrate their close relationship.Parmi les bacteriophages de Bacillus subtilis, certains ont la particularit6 ...

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Étude comparative des DNA de phages 2C, SP 8*, SP 82, φe, SP 01 et SP 50

Truffaut

Révet

Soulie

1970

European Journal of Biochemistry

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“…The assay measures both thymidylate synthetase and uridylate hydroxymethylase. The latter enzyme is found in cells infected by phage that contain hydroxymethyluracil in their DNA, such as 0e [8]. Phage-stimulated incorporation of uracil in minicells was followed by the method of Reeve and Comett [2].…”

Section: Methodsmentioning

confidence: 99%

Bacteriophage-coded specific enzyme synthesis in minicells of Bacillus subtilis

Jacobson

1978

FEMS Microbiology Letters

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“…Several Bacillus subtilis bacteriophages incorporate 5‐(hydroxymethyl)‐2'‐deoxyuridine (hmU) into their DNA genome in place of thymine as a natural nucleotide 1, 2. First noticed via its deviation from a linear relationship between base composition (% G + C) and melting temperature (T m ), hmU‐DNA was subsequently shown to be the cause of numerous genetic mutations and chromosomal instability 3, 4.…”

Section: Introductionmentioning

confidence: 99%

Specificity of hydroxylmethyluracil-containing DNA for transcription factor 1: Structural insights

Pasternack

Kearns

1999

Biopolymers

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The genomic materials from some Bacillus subtilis bacteriophages are found to contain 5‐(hydroxymethyl)‐2'‐deoxyuridine in place of thymine. Phage‐encoded proteins such as transcription factor 1 specifically and preferentially bind to the minor grooves of these hmU‐containing DNA but not to thymine‐containing DNA. Data from electrophoretic mobility shift assays suggest that the inherent, localized flexibility of hmU‐DNA, which is sequence‐specific, is responsible for its discriminative binding. We discuss here, from the NMR‐derived structural point of view, how differential DNA flexibility can contribute to specific binding of TF1 to hmU‐DNA. © 2000 John Wiley & Sons, Inc. Biopoly 52: 57–63, 1999

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The biosynthesis of 5-hydroxymethyldeoxyuridylic acid in bacteriophage-infected Bacillus subtilis

Cited by 66 publications

References 33 publications

Étude comparative des DNA de phages 2C, SP 8*, SP 82, φe, SP 01 et SP 50

Étude comparative des DNA de phages 2C, SP 8*, SP 82, φe, SP 01 et SP 50

Bacteriophage-coded specific enzyme synthesis in minicells of Bacillus subtilis

Specificity of hydroxylmethyluracil-containing DNA for transcription factor 1: Structural insights

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