The biosynthesis of <scp>l</scp>-rhamnose of plum-leaf polysaccharides

Andrews, P.; Hough, L.; Picken, J.M.

doi:10.1042/bj0970027

Cited by 21 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the molecular weight of fraction C was determined by gel filtration on Sephadex G-200. The column size was 2.5 X 50 cm and correlation was made between elution volume and molecular weight according to the method of Andrews (1965). An estimate of a molecular weight of 110,000 j= 11,ooO for subform C was calculated using gel filtration techniques which is in agreement with the value obtained by sucrose density gradient measurements.…”

Section: M)mentioning

confidence: 69%

Mitochondrial aspartate transaminase. II. Isolation and characterization of the multiple forms

Michuda¹,

Martinez‐Carrion²

1969

Biochemistry

View full text Add to dashboard Cite

Mitochondrial pig heart glutamate-aspartate transaminase (L-aspartate:2-oxoglutarate aminotransferase, E C 2.6.1.1) can be separated by ion-exchange chromatography on carboxymethyl-Sephadex into three fractions each possessing a different electrophoretic mobility. The absorption spectral properties of the fractions have been found to be similar. The subforms are equal in pyridoxal phosphate content, but differ in the relative amounts of "catalytically active" (absorbance at 430 mp at low pH and 360 mp at high pH) and "catalytically inactive" (absorbance at 340 mp at high or low pH) pyridoxal phosphate which accounts for differences in their specific activities and molecular mean residue ellipticity at 430 mp, The subforms display identical reaction rates with the pseudo-substrates, L-alanine, L-methionine, and L-serine. Their true dissociation constants with a-methylaspartate are equiva-

show abstract

Section: M)mentioning

confidence: 69%

Mitochondrial aspartate transaminase. II. Isolation and characterization of the multiple forms

Michuda¹,

Martinez‐Carrion²

1969

Biochemistry

View full text Add to dashboard Cite

show abstract

“…The Parthenoci8sus crown gall callus had both an abnormally high pectin content and an unusually large proportion of strongly acidic pectin. The rather high rhamnose content, relative to the other neutral sugars, is of interest in view of the probable presence of rhamnose in polygalacturonic acid chains (Andrews, Hough & Picken, 1965;Barrett & Northcote, 1965). The strongly acidic pectinic acid component seen at zone A in the electrophoretogram was always present in the pectins prepared from the actively dividing and growing callus and cambium tissue but it was difficult to detect and present only in trace amounts in the fruit pectin.…”

Section: Discussionmentioning

confidence: 99%

Pectic polysaccharides of growing plant tissues

Stoddart¹,

Barrett²,

Northcote³

1967

Biochemical Journal

120

View full text Add to dashboard Cite

1. The polysaccharide compositions of the cell walls of sycamore cambium and sycamore callus tissue have been analysed and found to be directly comparable. 2. Electrophoretic analyses of the whole pectins prepared from actively growing callus and cambial tissue have shown that these preparations contain, in addition to the neutral and weakly acidic components present in apple fruit, a strongly acidic polygalacturonic acid component. 3. The weakly acidic component of all the pectins was directly comparable with that of the pectinic acid of apple fruit. 4. The components of the whole pectin of sycamore callus tissue have been partially purified and analysed. The neutral and weakly acidic components also found in apple fruit were isolated. 5. The pattern of the composition of the neutral sugars present in the pectins of actively growing tissues of cambium and callus has been compared with those present in apple-fruit pectinic acid. 6. The presence of rhamnose linked as galacturonosyl-(1-->2)-rhamnose has been found in sycamore whole pectin. 7. The difference in the pectins of callus, cambium and fruit appears not to be that of species difference but is more characteristic of the nature of the growth and growth conditions of the cells. This is discussed in relation to the problems of the control and mechanism of plant-cell growth and differentiation.

show abstract

“…The molecular weight of the purified enzyme was estimated by calibrated Sephadex G-150 column chromatography according to the method of Andrews. 18) Chemicals. Glucose-6-phosphate and NADP+ were purchased from Sigma Chemical Co .. Ltd .. St. Louis.…”

Section: Deae-cellulose Column Chromatographymentioning

confidence: 99%