The biosynthetic products of Chinese insect medicine, Aspongopus chinensis

Xiao, Luo; Wang, Xiao Zheng; Jiang, Hai Long; Yang, Jianmei; Crews, Phillip; Valeriote, Frederick A.; Wu, Quan Xiang

doi:10.1016/j.fitote.2012.03.002

Cited by 39 publications

(25 citation statements)

References 10 publications

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“…The “Compendium of Materia Medica”, the ancient Chinese Pharmacopeia, records more than 100 medicinal insects. Currently, considerable research has investigated insect-derived medicines with the aim of providing scientific evidence for the insects’ proper utilization and modernized development [ 1 , 2 ]. China is one of the earliest countries to exploit insect resources in the world and has been a top producer of many insect-related industrial products for over one thousand years [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of the Defensive Secretion from the Medicinal Insect Blaps rynchopetera

et al. 2017

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Blaps rynchopetera Fairmaire has long been used as a folk medicine by the Yi and Bai ethnic groups in China to treat fever, cough, gastritis, boils, and tumors. In the present study, the cytotoxicity of the defensive secretion (TDS) of B. rynchopetera against AGS Caco-2, HepG2 U251 and Bel-7402 was tested, and the results revealed that TDS had potent cytotoxicity against testing cells with IC50 values of 45.8, 17.4, 53.6, 98.4 and 23.4 μg/mL, respectively. Gas chromatography-mass spectrometry (GC-MS) analysis was employed to clarify the cytotoxic constituents in TDS of B. rynchopetera and five volatile compounds, including 2-ethyl-2,5-cyclohexadiene-1,4-dione (3, 31.00%), 1-tridecene (5, 28.02%), 2-methyl-2,5-cyclohexadiene-1,4-dione (2, 22.86%), hydroquinone (4, 1.33%), and p-benzoquinone (1, 1.01%), were identified. Chemical constituent investigation on TDS further supported the presence of 5 above compounds. A cytotoxic assay indicated that compounds 1, 2, 3 and 4 exhibited significant cytotoxicity against the testing cell lines, implying that benzoquinones and hydroquinone played important roles in the cytotoxicity of TDS of B. rynchopetera. TDS is a cytotoxic natural material and further studies investigating mechanisms and inhibitory activities on other cell lines is warranted.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity of the Defensive Secretion from the Medicinal Insect Blaps rynchopetera

et al. 2017

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“…Dried A. chinensis is a traditional Chinese medicine utilised to treat pain, poor digestion, and kidney diseases. Previous investigations have shown that A. chinensis possesses pronounced antibacterial, anticancer, analgesic, antifibrotic, and angiogenesis-promoting activities [8–10, 12]. Our previous study revealed that the anticancer active components were mainly distributed in the haemolymph and abdomen [17] and that the haemolymph from A. chinensis can inhibit the proliferation of human gastric cancer cell lines SGC-7901 and BGC-823, and human breast cancer cell line MCF-7 in a dose- and time-dependent manner [18–20].…”

Section: Discussionmentioning

confidence: 99%

“…Aspongopus chinensis Dallas is an insect belonging to Pentatomidae and is widely distributed in China, including Guizhou, Sichuan, and Yunnan Provinces [8]. Dried A .…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative and Proapoptotic Effects of a Protein Component Purified from Aspongopus chinensis Dallas on Cancer Cells In Vitro and In Vivo

Tan

Tian

Cai

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Aspongopus chinensis Dallas is used as a traditional Chinese medicine. In China, clinical evidence suggests that it has anticancer activity. However, the anticancer active components are not fully elucidated. In the present study, we purified an anticancer active component (named CHP) from A. chinensis. To gain a comprehensive insight into the protein components, shotgun proteomic analysis was conducted. The anticancer active protein band was cut from the sodium dodecyl sulphate-polyacrylamide gel electrophoresis gel and digested with trypsin to generate peptide mixture. The peptide fragments were then analysed by liquid chromatography tandem mass spectrometry; 18 proteins were identified. In addition, we evaluated the effects of CHP on the proliferation and apoptosis of two human gastric cancer cell lines (SGC-7901 and BGC-823). The cultured cells were treated with CHP at concentrations of 20, 30, and 40 μg/mL. Inhibition of cell growth was determined by the MTT assay. Hoechst 33258 staining was adopted to detect apoptosis morphologically. Apoptotic cells were quantified by Annexin V-FITC/propidium iodide staining and flow cytometry. Tumour growth was assessed by subcutaneous inoculation of 4T1 cells into BALB/c mice. There was a concentration- and time-dependent decrease in the proliferation of both cell lines at CHP concentrations of 20–40 μg/mL. Apoptotic characteristics, such as karyopyknotic pyknic hyperfluorescence bolus and nuclear fragmentation, were observed in both the cell lines by Hoechst 33258 staining. Flow cytometry showed that CHP induced significant (P < 0.01) concentration-dependent apoptosis of SGC-7901 cells. In vivo assay showed that CHP can partially inhibit tumour growth derived from 4T1 cells in vivo. The present study is the first to report that CHP in A. chinensis inhibits the proliferation of cancer cell lines via the suppression of cancer cell proliferation and acceleration of apoptosis.

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“…For example, the stinkbug (Encosternum delegorguei) in Zimbabwe and South Africa decrease hypertension and have been used to cure asthma and heart diseases (27). The edible Chinese stinkbug, Aspongopus chinensis, has also been employed to relieve pain, and to treat nephropathy and kidney disease in China (28). The bedbug (Cimex lectularius and C. hemipterus) was employed in the treatment of urinary disorders, epilepsy, piles, alopecia, headache, constipation, ulcer, arthritis, hair loss, snake bites and to stop somnolence (29).…”

Section: Traditional Use and Ethnobiological Recordsmentioning

confidence: 99%