The Biotechnological Potential of Corynebacterium glutamicum, from Umami to Chemurgy

Vertès, Alain A.; Inui, Masayuki; Yukawa, Hideaki

doi:10.1007/978-3-642-29857-8_1

Cited by 12 publications

(5 citation statements)

References 276 publications

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“…[78]) is represented by the glutamate fermentation carried out in particular by Corynebacterium glutamicum [78][79][80]. Following the initial discovery of the microbial/fermentative production of glutamate [81], various empirical findings in the 1960s and 1970s [82,83] showed that a variety of treatments, involving biotin limitation, or the addition of weak surfactants such as 'acetylated corn oil' or Tween, or the use of certain auxotrophs, would enhance the efflux of glutamate in producer strains. Soon enough, however, it was recognised that this was not due to a general membrane-leakiness, because it was very selective for glutamate (and was even against a glutamate concentration gradient!…”

Section: Role Of Efflux Transporters In Metabolic Flux Controlmentioning

confidence: 99%

Control of metabolite efflux in microbial cell factories: current advances and future prospects

Kell¹

2018

Preprint

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The yield and ease of purification of biotechnological products are typically greatly enhanced if they can be secreted into the external medium. Although not universally appreciated, however, small molecule biotechnological products do not ‘float across’ the phospholipid bilayer portion of biological membranes, and thus they need transporters to assist their passage into the extramembrane and extracellular spaces. Some of these transporters may be reversible, equilibrative transporters that might more normally be used for uptake, while others may have an efflux directionality imposed on them via suitable energy coupling mechanisms. Despite the energetic costs of small molecule synthesis, natural evolution does in fact provide a number of mechanisms by which the secretion of such products can actually enhance fitness. Where available these provide useful starting points, and assaying for such activities is crucial. In particular, in a systems biology approach, we first need to identify such/suitable activities before we can seek to increase them. They may then be improved through promoter engineering, via manipulation of control elements, or by directed evolution of the transporter proteins themselves. Modern methods of synthetic biology provide enormous opportunities for all kinds of efflux transporter engineering; they are just beginning to be realised.

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Section: Role Of Efflux Transporters In Metabolic Flux Controlmentioning

confidence: 99%

Control of metabolite efflux in microbial cell factories: current advances and future prospects

Kell¹

2018

Preprint

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“…Some bacterial orders coped well with low temperatures (freeze–thaw applied) but preferred a low-nutrient environment (no biochar addition) ( Figure 8 , group C). Most bacteria belonging to those orders were identified in environments characterized by low temperatures [ 75 ] and possess metal resistance genes [ 109 , 110 ]. Within this group, Pseudomonadales are one of the best-recognized orders in trace element contaminated soils.…”

Section: Resultsmentioning

confidence: 99%

Effect of Biochar on Metal Distribution and Microbiome Dynamic of a Phytostabilized Metalloid-Contaminated Soil Following Freeze–Thaw Cycles

et al. 2022

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In the present paper the effectiveness of biochar-aided phytostabilization of metal/metalloid-contaminated soil under freezing–thawing conditions and using the metal tolerating test plant Lolium perenne L. is comprehensively studied. The vegetative experiment consisted of plants cultivated for over 52 days with no exposure to freezing–thawing in a glass greenhouse, followed by 64 days under freezing-–thawing in a temperature-controlled apparatus and was carried out in initial soil derived from a post-industrial urban area, characterized by the higher total content of Zn, Pb, Cu, Cr, As and Hg than the limit values included in the classification provided by the Regulation of the Polish Ministry of Environment. According to the substance priority list published by the Toxic Substances and Disease Registry Agency, As, Pb, and Hg are also indicated as being among the top three most hazardous substances. The initial soil was modified by biochar obtained from willow chips. The freeze–thaw effect on the total content of metals/metalloids (metal(-loid)s) in plant materials (roots and above-ground parts) and in phytostabilized soils (non- and biochar-amended) as well as on metal(-loid) concentration distribution/redistribution between four BCR (community bureau of reference) fractions extracted from phytostabilized soils was determined. Based on metal(-loid)s redistribution in phytostabilized soils, their stability was evaluated using the reduced partition index (Ir). Special attention was paid to investigating soil microbial composition. In both cases, before and after freezing–thawing, biochar increased plant biomass, soil pH value, and metal(-loid)s accumulation in roots, and decreased metal(-loid)s accumulation in stems and total content in the soil, respectively, as compared to the corresponding non-amended series (before and after freezing–thawing, respectively). In particular, in the phytostabilized biochar-amended series after freezing–thawing, the recorded total content of Zn, Cu, Pb, and As in roots substantially increased as well as the Hg, Cu, Cr, and Zn in the soil was significantly reduced as compared to the corresponding non-amended series after freezing–thawing. Moreover, exposure to freezing–thawing itself caused redistribution of examined metal(-loid)s from mobile and/or potentially mobile into the most stable fraction, but this transformation was favored by biochar presence, especially for Cu, Pb, Cr, and Hg. While freezing–thawing greatly affected soil microbiome composition, biochar reduced the freeze–thaw adverse effect on bacterial diversity and helped preserve bacterial groups important for efficient soil nutrient conversion. In biochar-amended soil exposed to freezing–thawing, psychrotolerant and trace element-resistant genera such as Rhodococcus sp. or Williamsia sp. were most abundant.

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“…The biotechnological potential of Corynebacterium glutamicum goes beyond amino acid synthesis and includes the targeted production of organic acids (lactate and succinate), alcohols and fuels (ethanol, butanol, isobutanol, and xylitol), aromatic compounds (phenol and terephthalate), biopolymers (polylactate, polysuccinate, and poly-(3-hydroxybutyrate)), polyamines (cadaverine and putrescine), industrial enzymes, and therapeutic proteins (Vertès et al 2013;Jojima et al 2013). Corynebacterium glutamicum has been studied also for a variety of bioremediation uses, mining purposes, and the biosorption of toxic heavy metals (Vertès et al 2013).…”

Section: Applicationmentioning

confidence: 99%

The Family Corynebacteriaceae

Tauch

Sandbote

2014

The Prokaryotes

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The Biotechnological Potential of Corynebacterium glutamicum, from Umami to Chemurgy

Cited by 12 publications

References 276 publications

Control of metabolite efflux in microbial cell factories: current advances and future prospects

Control of metabolite efflux in microbial cell factories: current advances and future prospects

Effect of Biochar on Metal Distribution and Microbiome Dynamic of a Phytostabilized Metalloid-Contaminated Soil Following Freeze–Thaw Cycles

The Family Corynebacteriaceae

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