The biotechnological potential of marine bacteria in the novel lineage of Pseudomonas pertucinogena

Abstract

Marine habitats represent a prolific source for molecules of biotechnological interest. In particular, marine bacteria have attracted attention and were successfully exploited for industrial applications. Recently, a group of Pseudomonas species isolated from extreme habitats or living in association with algae or sponges were clustered in the newly established Pseudomonas pertucinogena lineage. Remarkably for the predominantly terrestrial genus Pseudomonas, more than half (9) of currently 16 species within this lineage were isolated from marine or saline habitats. Unlike other Pseudomonas species, they seem to have in common a highly specialized metabolism. Furthermore, the marine members apparently possess the capacity to produce biomolecules of biotechnological interest (e.g. dehalogenases, polyester hydrolases, transaminases). Here, we summarize the knowledge regarding the enzymatic endowment of the marine Pseudomonas pertucinogena bacteria and report on a genomic analysis focusing on the presence of genes encoding esterases, dehalogenases, transaminases and secondary metabolites including carbon storage compounds.

A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri – Structural and Functional Insights

“…Obviously, a comprehensive comparative study of different PET degrading enzymes would be required. In case of PE-H, the marine origin of the producer P. aestusnigri suggests that natural substrates may be aliphatic polyesters produced by algae and plants, because marine bacteria of the P. pertucinogena lineage have been found associated with these organisms (Bollinger et al, 2020).…”

“…Additionally, we observed hydrolytic activity indicated by formation of clear halos upon growth of P. aestusnigri on agar plates containing Impranil DLN, an anionic aliphatic polyesterpolyurethane used for surface coating of textiles ( Figure 1A). Bioinformatic analysis of the P. aestusnigri genome sequence (Gomila et al, 2017) led us to predict the existence of a polyester hydrolase coding gene (Bollinger et al, 2020). The respective gene was cloned into a pET-22b(+) expression vector and produced by expression in E. coli BL21(DE3).…”

Agar plate‐based screening methods for the identification of polyester hydrolysis by Pseudomonas species

“…The fluorescent siderophore pyoverdine leads to a bright fluorescence of many Pseudomonas strains under UV light exposure. Production of fluorescent siderophores should not be an issue for libraries within E. coli or the strains of the P. pertucinogena lineage investigated here because of the absence of respective gene clusters (Bollinger et al ., ). However, if pyoverdine producers are the strains of interest, supplementing additional iron to the medium decreases the siderophore production and therefore the autofluorescence (Fig.…”

“…The recently established Pseudomonas pertucinogena lineage (Peix et al ., ) consists of several species barely explored until today. The group appears especially interesting for its distinct characteristics with respect to metabolism, genome size and, not least, habitats with very specific conditions including cold, high‐salt and chemically contaminated environments (Bollinger et al ., ). Remarkably for the predominantly terrestrial genus Pseudomonas , most of the species within this lineage were isolated from marine or saline habitats.…”

“…Remarkably for the predominantly terrestrial genus Pseudomonas , most of the species within this lineage were isolated from marine or saline habitats. Unlike other Pseudomonas species, which are well known for their versatile metabolism, bacteria of the P. pertucinogena lineage seem to have a more niche‐adapted metabolism in common (Bollinger et al ., ). This is indicated by a comparably small genome and a limited spectrum of utilizable carbon sources.…”