The Bisabolane Sesquiterpenoid Endoperoxide, 3,6-Epidioxy-1,10-bisaboladiene, Isolated fromCacalia delphiniifoliaInhibits the Growth of Human Cancer Cells and Induces Apoptosis

Sanguinarine as a Potent and Specific Inhibitor of Protein Phosphatase 2Cin Vitroand Induces ApoptosisviaPhosphorylation of p38 in HL60 Cells

“…HL60 cells were treated with 10 mg/ml of Hoechst 33258 (Dojindo Laboratories, Kumamoto, Japan) for 10 min at 37 C. Nuclear morphology was observed under a fluorescent microscope (excitation/emission wavelength ¼ 365/420 nm, BX51, Olympus Corp., Tokyo, Japan). 14) Gel electrophoresis. HL60 cells were seeded in a 24-well plate at a density of 5 Â 10 5 cells/ml.…”

Chemical Composition and Antioxidant/Antimicrobial Activities in Supercritical Carbon Dioxide Fluid Extract of Gloiopeltis tenax

“…Thujopsene has shown potent antibacterial activity, for example against Phytophthora ramorum at 2.0–3.0 ppm [21]. Inhaling vaporized Cedrol ((64.0 ± 7.7) × 10 −9 M) can affect autonomic nervous functions in humans, such as induce sedative effects, decrease heart rate and blood pressure [22], and (+)-cuparene, α-curcumene and (−)-β-bisabolene mostly exhibit a strong physiological activity [23,24]. Vanillylacetone and fitone both have some physical activity, especially vanillylacetone.…”

3,6‐Epidioxy‐1,10‐bisaboladiene inhibits G1‐specific transcription through Swi4/Swi6 and Mbp1/Swi6 via the Hog1 stress pathway in yeast

“…EDBD and a-curcumene were purified from C. delphiniifolia and ginger oil, respectively, and dissolved in MeOH, as described previously [1]. EDBD was subjected to chromatography via a silica-gel column using the organic layer extracted from C. delphiniifolia and then purified by reverse-phase HPLC on a Capcell Pak column (Shiseido Co., Ltd., Tokyo, Japan).…”

“…Because of its ease of genetic manipulation and other factors, the yeast Saccharomyces cerevisiae is an ideal model for the study of cell-cycle regulation and can therefore be used for the identification of novel antitumor agents, which typically affect cell-cycle processes. For example, we recently isolated a bisabolane sesquiterpene endoperoxide compound, 3,6-epidioxy-1,10-bisaboladiene (EDBD), from Cacalia delphiniifolia, an edible wild plant found in northern Japan [1], and using a screening assay in mutant yeast, found that this compound had antitumor activity. In this mutant model of S. cerevisiae, mutations are present in Rad9, which acts as a key adaptor protein in the DNA damage checkpoint pathway, and Cdc2, which acts as a catalytic subunit of DNA polymerase d [2,3].…”