The bladed Bangiales (Rhodophyta) of the South Eastern Pacific: Molecular species delimitation reveals extensive diversity

Guillemin, Marie‐Laure; Contreras–Porcia, Loretto; Ramirez, María Eliana; Macaya, Erasmo C.; Contador, Cristian Bulboa; Woods, Helen Buckley; Wyatt, Christopher D R; Brodie, Juliet

doi:10.1016/j.ympev.2015.09.027

Cited by 69 publications

(91 citation statements)

References 65 publications

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“…For the COI , 288 sequences were included in our data set: 90 newly obtained (i.e., first and second sampling scheme) from Maitencillo, 159 from Chilean Bangiales (including 20 from Maitencillo, Guillemin et al. ), and 39 from GenBank. The complete list of specimens used in molecular analyses is available in Tables and in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

“…The monophyly of each Pyropia and Porphyra species, previously determined by Guillemin et al. (), was examined using a maximum likelihood (ML) phylogenetic relationship reconstruction performed using IQ‐TREE online server (Trifinopoulos et al. ).…”

Section: Methodsmentioning

confidence: 99%

“…), tree reconstructions were done separately for Pyropia and Porphyra species, using as outgroups Boreophylum birdiae and Bangia fuscopurpurea , respectively, following Guillemin et al. () and Sutherland et al. ().…”

Section: Methodsmentioning

confidence: 99%

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Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Meynard

Zapata

Salas

et al. 2019

Journal of Phycology

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A recent molecular taxonomic study along the Chilean coast (18° S–53° S) described 18 candidate species of bladed Bangiales of which only two were formally described. Few studies focused on local genetic and morphological diversity of bladed Bangiales and attempted to determine their intertidal distribution in contrasting habitats, and none were performed in Chile. To delimit intertidal distributions of genetic species, 66 samples of bladed Bangiales were collected at Maitencillo (32° S) in four zones: a rocky platform, a rocky wall, and two boulders zones surrounded by sandy and rocky bottoms, respectively. These samples were identified based on sequences of the mitochondrial COI and chloroplast rbcL markers. We also collected 87 specimens for morphological characterization of the most common species, rapidly assessing their putative species identity using newly developed species‐diagnostic (PCR‐RFLP) markers. Eight microscopic and two macroscopic morphological traits were measured. We described and named three of four species that predominate in Maitencillo (including Pyropia orbicularis): Pyropia variabilis Zapata, Meynard, Ramírez, Contreras‐Porcia, sp. nov., Porphyra luchea Meynard, Ramírez, Contreras‐Porcia sp. nov., and Porphyra longissima Meynard, Ramírez, Contreras‐Porcia, sp. nov. With the exception of Po. longissima restricted to boulders surrounded by sandy bottom, and a morphotype of Py. variabilis restricted to rocky walls, the other species/morphotypes have overlapping intertidal distributions. Except for Po. longissima, which is clearly differentiated morphologically (longest and thinnest blades), we conclude that morphology is not sufficient to differentiate bladed Bangiales. Our findings underscore the importance of refining our knowledge of intrinsic and environmental determinants on the distribution of bladed Bangiales.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Meynard

Zapata

Salas

et al. 2019

Journal of Phycology

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“…The ecological success of Porphyra and many of the closely related bangiophyceans (37,41) in the intertidal zone suggests that these species developed cellular mechanisms to cope with this harsh environment. In particular, Porphyra grows from the mid-to-high intertidal zone, where it is routinely exposed during daily low tides to high light, desiccation, and extreme fluctuations in temperature and salinity.…”

Section: Significancementioning

confidence: 99%

Insights into the red algae and eukaryotic evolution from the genome of Porphyra umbilicalis (Bangiophyceae, Rhodophyta)

Brawley¹,

Blouin²,

Ficko-Blean³

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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238

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Porphyra umbilicalis (laver) belongs to an ancient group of red algae (Bangiophyceae), is harvested for human food, and thrives in the harsh conditions of the upper intertidal zone. Here we present the 87.7-Mbp haploid Porphyra genome (65.8% G + C content, 13,125 gene loci) and elucidate traits that inform our understanding of the biology of red algae as one of the few multicellular eukaryotic lineages. Novel features of the Porphyra genome shared by other red algae relate to the cytoskeleton, calcium signaling, the cell cycle, and stress-tolerance mechanisms including photoprotection. Cytoskeletal motor proteins in Porphyra are restricted to a small set of kinesins that appear to be the only universal cytoskeletal motors within the red algae. Dynein motors are absent, and most red algae, including Porphyra, lack myosin. This surprisingly minimal cytoskeleton offers a potential explanation for why red algal cells and multicellular structures are more limited in size than in most multicellular lineages. Additional discoveries further relating to the stress tolerance of bangiophytes include ancestral enzymes for sulfation of the hydrophilic galactan-rich cell wall, evidence for mannan synthesis that originated before the divergence of green and red algae, and a high capacity for nutrient uptake. Our analyses provide a comprehensive understanding of the red algae, which are both commercially important and have played a major role in the evolution of other algal groups through secondary endosymbioses.cytoskeleton | calcium-signaling | carbohydrate-active enzymes | stress tolerance | vitamin B 12T he red algae are one of the founding groups of photosynthetic eukaryotes (Archaeplastida) and among the few multicellular lineages within Eukarya. A red algal plastid, acquired through secondary endosymbiosis, supports carbon fixation, fatty acid synthesis, and other metabolic needs in many other algal groups in ways that are consequential. For example, diatoms and haptophytes have strong biogeochemical effects; apicomplexans cause human disease (e.g., malaria); and dinoflagellates include both coral symbionts and toxin-producing "red tides" (1). The evolutionary processes that produced the Archaeplastida and secondary algal lineages remain under investigation (2-5), but it is clear that both nuclear and plastid genes from the ancestral red algae have contributed dramatically to broader eukaryotic evolution and diversity. Consequently, the imprint of red algal metabolism on the Earth's climate system, aquatic foodwebs, and

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“…Recently, molecular tools for species delimitation have been used for the identification of many red algae (Guillemin et al 2016;Jesus et al 2016), including members of Polysiphonia sensu lato (Savoie & Saunders 2015). In this study, we carried out careful morphological observations of Polysiphonia scopulorum specimens from the type locality, Rottnest Island in Western Australia, along with molecular analyses using the rbcL gene to provide a more complete taxonomic treatment.…”

Section: Introductionmentioning

confidence: 99%

The phylogenetic position of Polysiphonia scopulorum (Rhodomelaceae, Rhodophyta) based on molecular analyses and morphological observations of specimens from the type locality in Western Australia

Huisman

Kim

2017

Phytotaxa

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Considerable uncertainty surrounds the phylogenetic position of Polysiphonia scopulorum, a species with an apparently cosmopolitan distribution. Here we report, for the first time, molecular phylogenetic analyses using plastid rbcL gene sequences and morphological observations of P. scopulorum collected from the type locality, Rottnest Island in Western Australia. Morphological characteristics of the Rottnest Island specimens allowed unequivocal identification, however, the sequence analyses uncovered discrepancies in previous molecular studies that included specimens identified as P. scopulorum from other locations. The phylogenetic evidence clearly revealed that P. scopulorum from Rottnest Island formed a sister clade with P. caespitosa from Spain (JX828149 as P. scopulorum) with moderate support, but that it differed from specimens identified as P. scopulorum from the U.S.A. (AY396039, EU492915). In light of this, we suggest that P. scopulorum be considered an endemic species with a distribution restricted to Australia. Our results showed the existence of several distinct clades among the species of Polysiphonia sensu lato, including the clade containing P. scopulorum which did not join with the generitype Polysiphonia stricta (i.e., Polysiphonia sensu stricto). This suggests that the P. scopulorum clade might represent a separate genus, however, further studies including multi-gene analyses are recommended before recognizing any segregate taxa.

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The bladed Bangiales (Rhodophyta) of the South Eastern Pacific: Molecular species delimitation reveals extensive diversity

Cited by 69 publications

References 65 publications

Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Insights into the red algae and eukaryotic evolution from the genome of Porphyra umbilicalis (Bangiophyceae, Rhodophyta)

The phylogenetic position of Polysiphonia scopulorum (Rhodomelaceae, Rhodophyta) based on molecular analyses and morphological observations of specimens from the type locality in Western Australia

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