The bone marrow peptide (myelopeptide-2) abolishes induced by human leukemia HL-60 cell suppression of T lymphocytes

Strelkov, L. A.; Mikhailova, A. V.; Sapozhnikov, Alexander M.; Fonina, L. A.; Петров, Р. В.

doi:10.1016/0165-2478(96)02527-8

Cited by 12 publications

(3 citation statements)

References 5 publications

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“…MPs have a wide spectrum of functional activities, including immunoregulatory, differentiating and opiate‐like (5). MP‐1 (Phe‐Leu‐Gly‐Phe‐Pro) and MP‐2 (Leu‐Val‐Val‐Tyr‐Pro) display pronounced immunoregulatory activity, whilst MP‐3 (Leu‐Val‐Cys‐Tyr‐Pro‐Gln) protects animals from bacterial infection (6–9).…”

Section: Introductionmentioning

confidence: 99%

Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOC_n): synthesis, conformation and studies in human leukemia cells

Keramisanou

Tsikaris

Sakarellos‐Daitsiotis

et al. 2002

The Journal of Peptide Research

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Myelopeptides, MP-6 (Val-Asp-Pro-Pro) and MP-4 (Phe-Arg-Pro-Arg-Ile-Met-Thr-Pro), induce metabolic changes in human leukemia cells, HL-60, characteristic of the differentiation process, which should be regarded as a promising therapeutic approach in cancer and related diseases. With the aim to optimize the differentiation effect of MPs, they were coupled to the Lys-N(epsilon)H(2) groups of a sequential oligopeptide carrier Ac-(Lys-Aib-Gly)(4), SOC(4), and the constructs obtained were studied. The rigid 3(10) secondary structure of the carrier is preserved even after linkage of the MPs, which also maintain their initial conformations without interacting either with each other or with the carrier, as demonstrated by (1)H nuclear magnetic resonance (NMR) spectroscopy. It is concluded that the carrier accommodates the presentation of MPs, thus improving their differentiation effect on human leukemia cells.

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Section: Introductionmentioning

confidence: 99%

Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOC_n): synthesis, conformation and studies in human leukemia cells

Keramisanou

Tsikaris

Sakarellos‐Daitsiotis

et al. 2002

The Journal of Peptide Research

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“…Earlier a group of bone marrow bioregulatory mediators was revealed [8,9]; they were called myelopeptides (MPs). MPs have a wide spectrum of functional activities: immunostimulating, immunoregulating and opiate‐like [8–12]. Several individual MPs (MP‐1, MP‐2, etc.)…”

Section: Introductionmentioning

confidence: 99%

“…Several individual MPs (MP‐1, MP‐2, etc.) responsible for these activities were originally isolated from the supernatant of porcine bone marrow cell culture medium and they were identified and synthesized [10–13]. The sequence of MP‐4 is known [13], but its function has not been established.…”

Section: Introductionmentioning

confidence: 99%

A new endogenous differentiating factor (myelopeptide‐4) for myeloid cells

et al. 2000

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Along with known lymphokines involved in the regulation of hematopoiesis, a new differentiating factor (myelopeptide-4, MP-4) for myeloid cells was found. The peptide (Phe-Arg-Pro-Arg-Ile-Met-Thr-Pro) originally isolated from the culture medium of porcine bone marrow cell culture was examined for its ability to induce differentiation in two human myeloid leukemia cell lines, HL-60 and K-562. Agents with wellknown differentiation-inducing activity, such as phorbol myristate acetate, dimethylsulfoxide and the lymphokines were used as a reference. It has been shown that MP-4 significantly influences the integral characteristics of metabolism, expression of surface antigens and morphology of these cells. It decreased the level of chromosomal DNA synthesis and, in parallel, increased the total protein synthesis in both HL-60 and K-562 cells. MP-4 induced the expression of CD14 monocyte-specific surface antigen and the appearance of mature monocytes/ macrophages in HL-60 cell cultures. There was a good correlation of cell metabolic/morphological changes and the CD14 marker expression for HL-60 cells. A similar phenomenon was observed in K-562 cells treated with MP-4 when the levels of hemoglobin synthesis were detected in their cytoplasm. Thus, we consider MP-4 as a new endogenous differentiating factor for myeloid cells.z 2000 Federation of European Biochemical Societies.

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Bone marrow immunoregulatory peptides (myelopeptides): Isolation, structure, and functional activity

1997

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The bone marrow peptide (myelopeptide-2) abolishes induced by human leukemia HL-60 cell suppression of T lymphocytes

Cited by 12 publications

References 5 publications

Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOC_n): synthesis, conformation and studies in human leukemia cells

Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOC_n): synthesis, conformation and studies in human leukemia cells

A new endogenous differentiating factor (myelopeptide‐4) for myeloid cells

Bone marrow immunoregulatory peptides (myelopeptides): Isolation, structure, and functional activity

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The bone marrow peptide (myelopeptide-2) abolishes induced by human leukemia HL-60 cell suppression of T lymphocytes

Cited by 12 publications

References 5 publications

Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOCn): synthesis, conformation and studies in human leukemia cells

Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOCn): synthesis, conformation and studies in human leukemia cells

A new endogenous differentiating factor (myelopeptide‐4) for myeloid cells

Bone marrow immunoregulatory peptides (myelopeptides): Isolation, structure, and functional activity

Contact Info

Product

Resources

About

Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOC_n): synthesis, conformation and studies in human leukemia cells

Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOC_n): synthesis, conformation and studies in human leukemia cells