The bone regeneration capacity of 3D-printed templates in calvarial defect models: A systematic review and meta-analysis

Hassan, Mohamad Nageeb; Yassin, Mohammed A.; Suliman, Salwa; Lie, Stein Atle; Gjengedal, Harald; Mustafa, Kamal

doi:10.1016/j.actbio.2019.04.017

Cited by 62 publications

(40 citation statements)

References 94 publications

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“…Three-dimensional (3D) printing technology has been developed to fabricate scaffolds with ideal geometries and structures exhibiting precise control of pore size, porosity, and pore morphology in order to treat large-scale bone defects [ [5] , [6] , [7] ]. However, for patients with DM, the local microenvironment at the region of the bone defect becomes inflamed, which can lead to vascular occlusion and reduced neovascularization, and because traditional 3D-printed scaffolds usually induce cell homing, local tissue responses, and functional stimulation, these scaffolds may use the host as a bioreactor to recruit host endogenous cells for tissue regeneration; therefore, the growth and viability of loaded cells is decreased in traditional 3D-printed scaffolds [ 5 , 6 , 8 , 9 ]. In addition, before in vivo transplantation, 3D-printed scaffolds require cell seeding and long-term cultivation in vitro, which increases the risk of infection and lengthens the time needed for surgery [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional bioprinting of multicell-laden scaffolds containing bone morphogenic protein-4 for promoting M2 macrophage polarization and accelerating bone defect repair in diabetes mellitus

Sun

Zhao

et al. 2021

Bioactive Materials

118

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Critical-sized bone defect repair in patients with diabetes mellitus remains a challenge in clinical treatment because of dysfunction of macrophage polarization and the inflammatory microenvironment in the bone defect region. Three-dimensional (3D) bioprinted scaffolds loaded with live cells and bioactive factors can improve cell viability and the inflammatory microenvironment and further accelerating bone repair. Here, we used modified bioinks comprising gelatin, gelatin methacryloyl (GelMA), and 4-arm poly (ethylene glycol) acrylate (PEG) to fabricate 3D bioprinted scaffolds containing BMSCs, RAW264.7 macrophages, and BMP-4-loaded mesoporous silica nanoparticles (MSNs). Addition of MSNs effectively improved the mechanical strength of GelMA/gelatin/PEG scaffolds. Moreover, MSNs sustainably released BMP-4 for long-term effectiveness. In 3D bioprinted scaffolds, BMP-4 promoted the polarization of RAW264.7 to M2 macrophages, which secrete anti-inflammatory factors and thereby reduce the levels of pro-inflammatory factors. BMP-4 released from MSNs and BMP-2 secreted from M2 macrophages collectively stimulated the osteogenic differentiation of BMSCs in the 3D bioprinted scaffolds. Furthermore, in calvarial critical-size defect models of diabetic rats, 3D bioprinted scaffolds loaded with MSNs/BMP-4 induced M2 macrophage polarization and improved the inflammatory microenvironment. And 3D bioprinted scaffolds with MSNs/BMP-4, BMSCs, and RAW264.7 cells significantly accelerated bone repair. In conclusion, our results indicated that implanting 3D bioprinted scaffolds containing MSNs/BMP-4, BMSCs, and RAW264.7 cells in bone defects may be an effective method for improving diabetic bone repair, owing to the direct effects of BMP-4 on promoting osteogenesis of BMSCs and regulating M2 type macrophage polarization to improve the inflammatory microenvironment and secrete BMP-2.

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Section: Introductionmentioning

confidence: 99%

Three-dimensional bioprinting of multicell-laden scaffolds containing bone morphogenic protein-4 for promoting M2 macrophage polarization and accelerating bone defect repair in diabetes mellitus

Sun

Zhao

et al. 2021

Bioactive Materials

118

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“…For bone regeneration, biomaterials are preferably manufactured three‐dimensionally with high porosity and interconnectivity to replicate bony structures in vivo 8 . However, the use of aliphatic polymers as three‐dimensional (3D) microporous scaffolds is problematic because of their hydrophobic nature.…”

Section: Introductionmentioning

confidence: 99%

Surface activation with oxygen plasma promotes osteogenesis with enhanced extracellular matrix formation in three‐dimensional microporous scaffolds

Yamada

Yassin

Weigel

et al. 2021

J Biomedical Materials Res

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Various types of synthetic polyesters have been developed as biomaterials for tissue engineering. These materials commonly possess biodegradability, biocompatibility, and formability, which are preferable properties for bone regeneration. The major challenge of using synthetic polyesters is the result of low cell affinity due to their hydrophobic nature, which hinders efficient cell seeding and active cell dynamics. To improve wettability, plasma treatment is widely used in industry. Here, we performed surface activation with oxygen plasma to hydrophobic copolymers, poly(l‐lactide‐co‐trimethylene carbonate), which were shaped in 2D films and 3D microporous scaffolds, and then we evaluated the resulting surface properties and the cellular responses of rat bone marrow stem cells (rBMSC) to the material. Using scanning electron microscopy and Fourier‐transform infrared spectroscopy, we demonstrated that short‐term plasma treatment increased nanotopographical surface roughness and wettability with minimal change in surface chemistry. On treated surfaces, initial cell adhesion and elongation were significantly promoted, and seeding efficiency was improved. In an osteoinductive environment, rBMSC on plasma‐treated scaffolds exhibited accelerated osteogenic differentiation with osteogenic markers including RUNX2, osterix, bone sialoprotein, and osteocalcin upregulated, and a greater amount of collagen matrix and mineral deposition were found. This study shows the utility of plasma surface activation for polymeric scaffolds in bone tissue engineering.

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“…A new tools represented by 3D printing [46,47] may be of great help as well. Few studies show what can be done even for bone would be useful for the astronauts in case of broken legs it will help to ix the bone....…”

Section: Figurementioning

confidence: 99%

The place of health innovation in space to improve the lives of earthlings

Godard¹

2019

Ann Civil Environ Eng

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The bone regeneration capacity of 3D-printed templates in calvarial defect models: A systematic review and meta-analysis

Cited by 62 publications

References 94 publications

Three-dimensional bioprinting of multicell-laden scaffolds containing bone morphogenic protein-4 for promoting M2 macrophage polarization and accelerating bone defect repair in diabetes mellitus

Three-dimensional bioprinting of multicell-laden scaffolds containing bone morphogenic protein-4 for promoting M2 macrophage polarization and accelerating bone defect repair in diabetes mellitus

Surface activation with oxygen plasma promotes osteogenesis with enhanced extracellular matrix formation in three‐dimensional microporous scaffolds

The place of health innovation in space to improve the lives of earthlings

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