The bovine immune response to tick-derived Babesia Bovis infection: Serological studies of isolated immunoglobulins

Goff, Will L.; Wagner, G. G.; Craig, Thomas M.; Long, R.F.

doi:10.1016/0304-4017(82)90032-2

Cited by 41 publications

(22 citation statements)

References 18 publications

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“…In addition, using field samples, its performance was compared with that of the currently accepted gold standard, IFAT. The results demonstrate the applicability of rMSA-2c cELISA in the field for the diagnosis of cattle naturally infected with B. bovis in Argentina (22,32).…”

mentioning

confidence: 65%

“…A number of serological methods have been established for the detection of B. bovis-specific antibodies, of which ELISA is considered the most advantageous for epidemiological investigations, as it offers greater sensitivity and objectivity and can be easily adapted to test large numbers of serum samples (3,4,6,7,8,11,20,21,27,31,35,37). Furthermore, this format is amenable to the use of recombinant antigens, which makes it independent of the necessity to produce large amounts of parasites in vivo or in vitro, facilitates standardization, and has the potential to overcome limitations caused by cross-reactivity (22,32). The use of rMSA-2c as a diagnostic antigen in an indirect-ELISA format has been reported, and it has proved useful in the study of the development of antibody titers after experimental infection of bovines (6).…”

Section: Resultsmentioning

confidence: 99%

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Validation and Field Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay for Diagnosis of Babesia bovis Infections in Argentina

Dominguez

Echaide²,

Echaide³

et al. 2012

Clin Vaccine Immunol

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ABSTRACTInfections byBabesia bovislimit cattle production and cause important economic losses in tropical and subtropical areas around the world. Monitoring of calf sera can be used to detect unprotected cattle herds and to decide on strategic control measures, as well as for epidemiological studies. Merozoite surface antigen 2c (MSA-2c) is an immunodominant surface protein expressed inB. bovismerozoites and sporozoites and contains B-cell epitopes that are conserved among geographic isolates. A monoclonal antibody against recombinant MSA-2c (rMSA-2c) was previously shown to inhibit the binding of anti-B. bovisantibodies to a parasite B-cell epitope in a competitive enzyme-linked immunosorbent assay (cELISA) format. In the work at hand, the parameters of this cELISA were reevaluated and adjusted when necessary, and a cutoff value was determined by receiver operator characteristic (ROC) curve analysis of a total of 357 bovine sera of known reactivity, as assessed by indirect immunofluorescence assay (IFAT). The established rMSA-2c cELISA demonstrated a specificity of 98% and a sensitivity of 96.2%. An additional set of 303 field bovine sera from regions where ticks are endemic and tick-free regions of Argentina was tested by both rMSA-2c cELISA and IFAT, and the results were shown to be in very good agreement (kappa index, 0.8325). The performance shown by rMSA-2c cELISA in the detection ofB. bovis-specific antibodies and its suitability for standardization and large-scale production, as well as the possibility of its application in most veterinary diagnostic laboratories, make the assay a powerful tool for the surveillance of herd immunity as a strategic measure for the control of bovine babesiosis.

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confidence: 65%

Section: Resultsmentioning

confidence: 99%

Validation and Field Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay for Diagnosis of Babesia bovis Infections in Argentina

Dominguez

Echaide²,

Echaide³

et al. 2012

Clin Vaccine Immunol

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“…2, 3). In other studies it has been shown that following B. bovis infection, IgG1, but not IgG2, complement-fixing antibodies are detected [32] and antibody-dependent cell-mediated cytotoxicity may be involved in the resolution of B. bovis infection in cattle [33].…”

Section: Discussionmentioning

confidence: 94%

Co-immunization of cattle with a vaccine against babesiosis and Lactobacillus casei increases specific IgG1 levels to Babesia bovis and B. bigemina

Garfias

Lozano

Martı́nez

et al. 2015

Parasitology International

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“…However, parasitemias are often too low in subclinical and chronic infections for microscopic diagnosis. For this reason, several serologic assays, including complement fixation (14), indirect hemagglutination (7), rapid card agglutination (23), latex bead agglutination (12), and indirect immunofluorescence (IIF) (4) have been used to detect antibody in infected cattle. Although these assays allow for the detection of persistently infected cattle, they have limitations in specificity and/or sensitivity.…”

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confidence: 99%

Competitive Enzyme-Linked Immunosorbent Assay Based on a Rhoptry-Associated Protein 1 Epitope Specifically Identifies Babesia bovis -Infected Cattle

Goff

McElwain

Suárez

et al. 2003

Clin Vaccine Immunol

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The competitive enzyme-linked immunosorbent assay (cELISA) format has proven to be an accurate, reliable, easily standardized, and high-throughput method for detecting hemoparasite infections. In the present study, a species-specific, broadly conserved, and tandemly repeated B-cell epitope within the C terminus of the rhoptry-associated protein 1 of the hemoparasite Babesia bovis was cloned and expressed as a histidine-tagged thioredoxin fusion peptide and used as antigen in a cELISA. The assay was optimized with defined negative and positive bovine sera, where positive sera inhibited the binding of the epitope-specific monoclonal antibody BABB75A4. The cELISA accurately differentiated animals with B. bovis-specific antibodies from uninfected animals and from animals with antibodies against other tick-borne hemoparasites (98.7% specificity). In addition, B. bovis-specific sera from Australia, Argentina, Bolivia, Puerto Rico, and Morocco inhibited the binding of BABB75A4, confirming conservation of the epitope. The assay first detected experimentally infected animals between 13 and 17 days postinfection, and with sera from naturally infected carrier cattle, was comparable to indirect immunofluorescence (98.3% concordance). The assay appears to have the characteristics necessary for an epidemiologic and disease surveillance tool.

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The bovine immune response to tick-derived Babesia Bovis infection: Serological studies of isolated immunoglobulins

Cited by 41 publications

References 18 publications

Validation and Field Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay for Diagnosis of Babesia bovis Infections in Argentina

Validation and Field Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay for Diagnosis of Babesia bovis Infections in Argentina

Co-immunization of cattle with a vaccine against babesiosis and Lactobacillus casei increases specific IgG1 levels to Babesia bovis and B. bigemina

Competitive Enzyme-Linked Immunosorbent Assay Based on a Rhoptry-Associated Protein 1 Epitope Specifically Identifies Babesia bovis -Infected Cattle

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