The brains of the bones: how osteocytes use WNT1 to control bone formation

Rauch, Frank

doi:10.1172/jci95386

Cited by 10 publications

(11 citation statements)

References 15 publications

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“…We also introduced new parameters to quantify the local porosity of the lacunae and canaliculi within their corresponding Voronoi cells. The average volume of each Voronoi cell (Cell.V) was found to be 26000 µm 3 . The average volume of canaliculi per cell (Ca.V) at 30 nm voxel size was 115.3 µm 3 .…”

Section: Discussionmentioning

confidence: 96%

“…Image analysis was performed using custom programs. All processing steps were applied to the entire (2048) 3 volumes.…”

Section: Methodsmentioning

confidence: 99%

“…1(c)). Using these offsets, we cut a 512 3 in the sample and has the same field of view of 61.44 μm ( Fig. 1(d)).…”

Section: Methodsmentioning

confidence: 99%

“…As endocrine cells, they could have an impact on many organs 1 . For example, osteocytes could be regulators of bone resorption by secreting Receptor activator of NF-κB ligand (RANKL) 2 and control bone formation by producing WNT1 3 . Apart from their function in bone homeostasis, osteocytes may also contribute to fat metabolism by secreting sclerostin, leading to the increase of beige adipogenesis 4 , and influence hematopoiesis by the adjustment of the endosteal microenvironment through the release of soluble factors 5 .…”

mentioning

confidence: 99%

“…(c) The location of the maximum of the result provides the translation between the two images and permits to locate the common area (red square). (d) The 120 nm image is cropped to 5123 giving the same VOI than the 30 nm.…”

mentioning

confidence: 99%

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Assessment of the human bone lacuno-canalicular network at the nanoscale and impact of spatial resolution

Pacureanu

Olivier

et al. 2020

Sci Rep

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cécile olivier 1,2 , peter cloetens 2 & françoise peyrin 1,2* Recently, increasing attention has been given to the study of osteocytes, the cells that are thought to play an important role in bone remodeling and in the mechanisms of bone fragility. the interconnected osteocyte system is deeply embedded inside the mineralized bone matrix and lies within a closely fitted porosity known as the lacuno-canalicular network. However, quantitative data on human samples remain scarce, mostly measured in 2D, and there are gaps to be filled in terms of spatial resolution. In this work, we present data on femoral samples from female donors imaged with isotropic 3D spatial resolution by magnified X-ray phase nano computerized-tomography. We report quantitative results on the 3D structure of canaliculi in human femoral bone imaged with a voxel size of 30 nm. We found that the lacuno-canalicular porosity occupies on average 1.45% of the total tissue volume, the ratio of the canalicular versus lacunar porosity is about 37.7%, and the primary number of canaliculi stemming from each lacuna is 79 on average. The examination of this number at different distances from the surface of the lacunae demonstrates branching in the canaliculi network. We analyzed the impact of spatial resolution on quantification by comparing parameters extracted from the same samples imaged with 120 nm and 30 nm voxel sizes. To avoid any bias related to the analysis region, the volumes at 120 nm and 30 nm were registered and cropped to the same field of view. Our results show that the measurements at 120 and 30 nm are strongly correlated in our data set but that the highest spatial resolution provides more accurate information on the canaliculi network and its branching properties.

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Section: Discussionmentioning

confidence: 96%

“…Image analysis was performed using custom programs. All processing steps were applied to the entire (2048) 3 volumes.…”

Section: Methodsmentioning

confidence: 99%

“…1(c)). Using these offsets, we cut a 512 3 in the sample and has the same field of view of 61.44 μm ( Fig. 1(d)).…”

Section: Methodsmentioning

confidence: 99%