The breakdown of bilayer lipid membranes by dendrimers

Shcharbin, Dzmitry; Drapeza, A. I.; Loban, V. A.; Lisichenok, Alexej; Bryszewska, Maria

doi:10.2478/s11658-006-0018-2

Cited by 32 publications

(20 citation statements)

References 20 publications

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“…The destabilization of the lipidic part of biological membranes has been investigated in several models of liposomes. Some dendrimers are able to remove lipids and create nanoholes in the structure of the lipid bilayer [39][40][41]. It has been shown that dendrimers change the properties of the lipid bilayer not only on the surface but also in the hydrophobic region [28,29,42,43].…”

Section: Discussionmentioning

confidence: 99%

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Wrobel

Appelhans

Signorelli

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The influence of maltose-modified poly(propylene imine) (PPI) dendrimers on dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (DMPC/DMPG) (3%) liposomes was studied. Fourth generation (G4) PPI dendrimers with primary amino surface groups were partially (open shell glycodendrimers - OS) or completely (dense shell glycodendrimers - DS) modified with maltose residues. As a model membrane, two types of 100nm diameter liposomes were used to observe differences in the interactions between neutral DMPC and negatively charged DMPC/DMPG bilayers. Interactions were studied using fluorescence spectroscopy to evaluate the membrane fluidity of both the hydrophobic and hydrophilic parts of the lipid bilayer and using differential scanning calorimetry to investigate thermodynamic parameter changes. Pulsed-filed gradient NMR experiments were carried out to evaluate common diffusion coefficient of DMPG and DS PPI in D2O when using below critical micelle concentration of DMPG. Both OS and DS PPI G4 dendrimers show interactions with liposomes. Neutral DS dendrimers exhibit stronger changes in membrane fluidity compared to OS dendrimers. The bilayer structure seems more rigid in the case of anionic DMPC/DMPG liposomes in comparison to pure and neutral DMPC liposomes. Generally, interactions of dendrimers with anionic DMPC/DMPG and neutral DMPC liposomes were at the same level. Higher concentrations of positively charged OS dendrimers induced the aggregation process with negatively charged liposomes. For all types of experiments, the presence of NaCl decreased the strength of the interactions between glycodendrimers and liposomes. Based on NMR diffusion experiments we suggest that apart from electrostatic interactions for OS PPI hydrogen bonds play a major role in maltose-modified PPI dendrimer interactions with anionic and neutral model membranes where a contact surface is needed for undergoing multiple H-bond interactions between maltose shell of glycodendrimers and surface membrane of liposome.

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Section: Discussionmentioning

confidence: 99%

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Wrobel

Appelhans

Signorelli

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…the rupture, were evaluated based on current-voltage characteristics using the relevant plots [9,10] (Fig. 1).…”

Section: Formation Of Planar Lipid Membranesmentioning

confidence: 99%

“…A customized multifunctional BLM-system, in which two chambers of a Teflon cell, each containing a platinized electrode, are separated by a septum with the orifice of 1.5 mm, was used to study the current-voltage (V-A) characteristics up to the picoampere level, as well as to monitor the kinetics of electric conductivity of planar BLMs [9,10]. The device allows measuring of the penetration of acid/alkaline ions (like SA or ASA) through a lipid bilayer, which is stabilized by the constant electric field of -30 /-20 mV.…”

Section: System For the Study Of Current-voltage Characteristics Of Pmentioning

confidence: 99%

“…Since it is possible to modulate both voltage and current (amperage), the device also ensures the possibility of analyzing the resistance of the lipid bilayer, which appears linear as long as the bilayer remains intact, while the formation of holes and pores in the bilayer results in the appearance of non-linear regions. In addition, the fluctuations in lipid composition of a bilayer may affect V-A characteristics, i.e., the formation of holes or pores is strongly influenced by varying cholesterol concentration [9,10]. The formation of the BLM over the orifice was monitored on the plot of the current versus a sweeping membrane potential, as well as by means of a microvideo system, which enabled observation of the membrane formation in a real-time The data analysis of all the acquired signals -current-voltage characteristics, pH and video signals -was performed by means of the in-house software [9,10].…”

Section: System For the Study Of Current-voltage Characteristics Of Pmentioning

confidence: 99%

“…In addition, the fluctuations in lipid composition of a bilayer may affect V-A characteristics, i.e., the formation of holes or pores is strongly influenced by varying cholesterol concentration [9,10]. The formation of the BLM over the orifice was monitored on the plot of the current versus a sweeping membrane potential, as well as by means of a microvideo system, which enabled observation of the membrane formation in a real-time The data analysis of all the acquired signals -current-voltage characteristics, pH and video signals -was performed by means of the in-house software [9,10]. In the used BLM system, there were two principal components of current flowing through the lipid bilayer: the charging current, I C , and the ohmic current, I R .…”

Section: System For the Study Of Current-voltage Characteristics Of Pmentioning

confidence: 99%

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Effect of acetylsalicylic acid on the current–voltage characteristics of planar lipid membranes

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. contributed to the formation of metastable single pores, which facilitated ASA diffusion through a bilayer. Our data point out that ASA transport across the lipid bilayer takes place predominantly via the process of passive diffusion. In conclusion, the effects of ASA on lipid bilayer stability may contribute to drug transport through membrane lipid bilayers. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

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Polymers under Confinement

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The breakdown of bilayer lipid membranes by dendrimers

Cited by 32 publications

References 20 publications

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Effect of acetylsalicylic acid on the current–voltage characteristics of planar lipid membranes

Polymers under Confinement

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