The C-159t Polymorphism of the Cd-14 Gene and Cytokine Profiles in Adults With Atopic Dermatitis

Litus, Oleksandr; Derkach, N.; Litus, Viktor; Bisyuk, Yuriy; DuBuske, L.

doi:10.1016/j.anai.2018.09.054

Cited by 1 publication

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“…Yazdani et al revealed that the CC genotype is mainly related to allergic asthma risk 17. According to a study conducted on a Ukrainian population, the carriers of the C allele at the polymorphic region C-159T of the CD14 gene were at increased risk of atopic dermatitis compared with carriers of the T allele [ 11 ]. The study also revealed an association between the CD14 gene polymorphism C-159T and atopic disease [ 24 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…A C-to-T transition was identified in the promoter region of this gene at location 159. This vital polymorphism C-159T was associated with a variety of allergic diseases [ 11 ]. In this context, to our knowledge, this is the first study to investigate the association between serum level of sCD14 and CD14 gene polymorphism C-159T as well as AR disease in a group of the Egyptian population.…”

Section: Introductionmentioning

confidence: 99%

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Association of serum CD14 level and functional polymorphism C-159T in the promoter region of CD14 gene with allergic rhinitis

Kamel,

Selim,

Tantawy

et al. 2023

Clin Exp Med

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Allergic rhinitis (AR) is an inflammatory disease of the upper respiratory tract affecting a significant number of the world’s population. It occurs as an IgE-mediated immune response of the nasal mucosa to inhaled allergens. The human Cluster of Differentiation 14 (CD14) is a glycosyl-phosphatidylinositol-anchored molecule expressed on the surface of monocytes and macrophages and functions as a receptor to lipopolysaccharides and inhaled endotoxins that may stimulate interleukins production by antigen-presenting cells. Consequently, CD14 plays a substantial role in allergic diseases and may become one of their etiological causes. Allergic rhinitis (AR) is an inflammatory disease of the upper respiratory tract affecting a significant number of the world’s population. It occurs as an IgE-mediated immune response of the nasal mucosa to inhaled allergens. The human Cluster of Differentiation 14 (CD14) is a glycosyl-phosphatidylinositol-anchored molecule expressed on the surface of monocytes and macrophages and functions as a receptor to lipopolysaccharides and inhaled endotoxins that may stimulate interleukins production by antigen-presenting cells. Consequently, CD14 plays a substantial role in allergic diseases and may become one of their etiological causes. This study aimed to determine the association between C-159T polymorphism in the CD14 gene promoter region and serum CD14 levels and the risk of Allergic rhinitis Egyptian patients and to test the validity of serum CD14 level measurement in predicting AR. This case–control study included 45 patients with AR referred to Allergy and Immunology Unit, Zagazig University Hospital, Zagazig, Egypt, and 45 healthy subjects as controls. Serum CD14 levels were measured by ELISA. The polymerase chain reaction-restriction fragment length polymorphism technique was used to detect C-159T gene polymorphism in the CD14 promoter region. This case–control study included 45 patients with AR referred to Allergy and Immunology Unit, Zagazig University Hospital, Zagazig, Egypt, and 45 healthy subjects as controls. Serum CD14 levels were measured by ELISA. The polymerase chain reaction-restriction fragment length polymorphism technique was used to detect C-159T gene polymorphism in the CD14 promoter region. There was a significant association between CD14 serum levels and AR incidence (P < 0.001), with patients having higher serum CD14 levels than controls. In addition, a significant association (P < 0.001) was detected between serum CD14 levels and the severity of AR, as well as elevated serum CD14 levels in severe and the most severe cases. On the molecular level, there was a statistically significant relationship between patients and the control group regarding the CD14 genotype (P < 0.001), where CT and TT genotypes and T allele were primarily associated with the cases group, indicating that the risk of AR was significantly associated with the inheritance of the TT genotype. Additionally, a statistically significant association was found between the severity of AR and CD14 genotype (P < 0.001), where TT genotypes were mainly associated with severe and the most severe cases. In the studied groups, there was a statistically significant difference (P < 0.05) between the CD14 genotype and serum CD14 levels, with TT genotypes being associated with higher CD14 levels. The results obtained in this study revealed that serum CD14 level is a potential biomarker for the diagnosis of AR and, at the genetic level, a potential predictor of disease.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%