The C-3 Epimer of 25-Hydroxyvitamin D<sub>3</sub>Is Present in Adult Serum

Lensmeyer, Gary L.; Poquette, Michael A.; Wiebe, Donald A.; Binkley, Neil

doi:10.1210/jc.2011-0584

Cited by 161 publications

(139 citation statements)

References 18 publications

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“…The different allele forms of DBP circulate at varying concentrations and possess different binding affinities for 25(OH)D and 1,25(OH) 2 D; therefore, both these variables have the potential to influence bioavailability of vitamin D (44). These data are in accordance with the recent genome-wide association studies showing that lower affinity forms of DBP are associated with lower circulating levels of 25(OH)D so that the affinity of the binding may regulate both the total and free 25(OH)D levels (33,45) (Fig. 2).…”

Section: Measurement Of 25(oh)dsupporting

confidence: 86%

“…Indeed, in one of the most recent papers addressing the problem of the optimal threshold for defining vitamin D status, the authors performed the measurement of 25(OH)D in a blood sample taken at autopsy; they stated that, unlike PTH and calcium, 25(OH)D was found to be stable in various experiments for at least 10 days postmortem (22 (25), poses an individual treated with D 2 at risk of vitamin D intoxication, because with some assays he/she will unlikely reach the 'laboratory' sufficiency. Therefore, according to the authorities in the field (19,26) epimer which is a related molecule present in varying concentrations in normal subjects (33) that may interfere with the results obtained by LC-MS/MS. Another possible interference could derive from isobars, even though more detailed investigations are needed concerning these substances (19,34).…”

Section: Measurement Of 25(oh)dmentioning

confidence: 99%

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MANAGEMENT OF ENDOCRINE DISEASE: Value and limitations of assessing vitamin D nutritional status and advised levels of vitamin D supplementation

Romagnoli

Pepe

Piemonte

et al. 2013

European Journal of Endocrinology

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The growing attention to the role of vitamin D in skeletal and extra-skeletal diseases over the last decade induced an increased demand for vitamin D determination as well as a dramatic rise of sales of vitamin D supplement. However, several critical points in this field remain to be clarified. We lack a clear consensus about the definition of vitamin D deficiency, insufficiency, and sufficiency. The identification of different thresholds defining vitamin D status has relevant implications in clinical practice. In fact, the worldwide prevalence of low vitamin D status is highly varying according to the level of 25(OH)D utilized to define sufficiency. Therefore, the assessment of 25-hydroxyvitamin D levels may have a critical role, but a number of different technical problems associated with its determination may interfere in interpreting the results. The hydrophobic nature of vitamin D and the tight binding to its carrier (vitamin D binding protein), the different forms circulating in blood, and the issue of standardization are among the most important factors influencing the measurement of this metabolite. Another controversial point relies on the conflicting guidance on prevention and treatment of vitamin D deficiency endorsed by different medical and scientific communities. In particular, uncertainty exists about how to replete vitamin D stores, how to maintain normal 25(OH)D levels after repletion, which form of vitamin D is preferable for supplementation, and which route of administration and dosing regimens are advisable. Finally, concerns have been raised regarding vitamin D toxicity and its adverse effects.

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Section: Measurement Of 25(oh)dsupporting

confidence: 86%

Section: Measurement Of 25(oh)dmentioning

confidence: 99%

MANAGEMENT OF ENDOCRINE DISEASE: Value and limitations of assessing vitamin D nutritional status and advised levels of vitamin D supplementation

Romagnoli

Pepe

Piemonte

et al. 2013

European Journal of Endocrinology

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“…Finally, the C-3 epimer, 3-epi-25(OH)D, will also give overestimated 25(OH)D concentrations if not properly resolved by chromatography (vide infra) (7,15,16 (17 ). PTAD has been shown to exhibit limited stability (18 ); improved versions were reported by Higashi et al (17 ).…”

Section: Limitations Of Lc-ms/ms Assaysmentioning

confidence: 99%

Mass Spectrometric Profiling of Vitamin D Metabolites beyond 25-Hydroxyvitamin D

Müller

Volmer

2015

Clinical Chemistry

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BACKGROUND:The frequency of measurements of vitamin D in the human population has significantly increased over the last decade because vitamin D has now been linked to many diseases, in addition to its established role in bone health. Usually, serum 25-hydroxyvitamin D concentrations are measured to assess the vitamin D status of individuals. Unfortunately, many studies investigating links between vitamin D and disease also use only this single metabolite. Intricate correlations with other vitamin D metabolites or dynamic effects of downstream metabolites may therefore be overlooked. Fortunately, powerful LC-MS/MS approaches have recently become available that can simultaneously quantify the concentrations of multiple vitamin D metabolites. These approaches are challenging, however, because of inherent instrumental problems with detection of vitamin D compounds and the low concentrations of the metabolites in biological fluids.CONTENT: This review summarizes recent mass spectrometry assays for the quantitative measurement of multiple vitamin D metabolites and their application in clinical research, with a particular focus on the lowabundance downstream metabolic species generated after the initial hydroxylation to 25-hydroxyvitamin D.

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“…None of the main immunoassays in use today are susceptible to 3-epi-25-OHD 3 interference (14). Although the amounts of vitamin D epimer in adult serum are generally small, high concentrations have been reported in some individuals (36).…”

Section: Choice Of Methodsmentioning

confidence: 99%

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

Babić¹

2012

Journal of Medical Biochemistry

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Summary: Demand for vitamin D testing has been on a constant rise worldwide, partially due to mounting evidence linking vitamin D status to overall health and well-being. Currently available assays measure 25-hydroxy vitamin D (25-OHD), a major circulating form of vitamin D. Available methodologies include immunoassays and mass spectrometry based methods (LC-MS/MS). Until recently, the only immunoassays available for diagnostic use in the US have been DiaSorin radioimmunoassay (RIA) and an automated immunoassay on a LIAISON ® platform. Within the last year, Siemens and Abbott successfully launched immunoassays for determination of total vitamin D on their respective automated platforms, Centaur ® and ARCHITECT ® . Development of robust and precise Vitamin D immunoassays has historically been plagued with difficulty. One of the major challenges is development of specific antibodies against such a small antigen. Vitamin D is also highly hydrophobic molecule predominantly bound to vitamin D binding protein (DBP). It is likely, therefore, that immunoassays might be affected to varying extent by the DBP concentration. Adoption of LC-MS/MS into clinical laboratories has enabled development of accurate and almost fully automated methods that could handle increasing volume demands, especially in large volume reference laboratories. Smaller to mid-size hospital laboratories as well as physician offices have neither funds nor technical expertise to implement LC-MS/MS based testing. Our laboratory at the University of Chicago Medical Center has also seen increase in vitamin D volume and currently performs close to 20,000 25-OHD assays per year. We have recently developed an LC-MS/MS method for quantitation of 25-OHD 2 (obtained from plant sources) and 25-OHD 3 (endogenous and animal sources). Prior to acquisition of LC-MS/MS Kratak sadr`aj: Broj zahteva za odre|ivanjem vitamina D je u konstantnom porastu {irom sveta, delom zbog sve vi{e dokaza koji povezuju status vitamina D sa op{tim zdravljem. Trenutno raspolo`ivim testovima odre|uje se 25-hidroksi vitamin D (25-OHD), glavni oblik vitamina D u cirkulaciji. Po stoje}e metodologije uklju~uju imunometrijska odre |i vanja i tehnike zasnovane na masenoj spektrometriji (LC-MS/MS). Do nedavno, jedine raspolo`ive imunometrijske metode kori{}ene za dijagnostiku u SAD su bile DiaSorin radioimunoodre|ivanje (RIA) i automatizovano imuno odre|ivanje na LIAISON ® platformi. U toku pro{le godine Siemens i Abbott su uspe{no lansirali imunometrijske testove za odre|ivanje ukupnog vitamina D na svojim odgova raju}im automatizovanim platformama, Centaur ® i ARCHI-TECT ® . Razvoj robusnih i preciznih imunometrijskih testova za odre|ivanje vitamina D su, istorijski gledano, pratili problemi. Jedan od najve}ih izazova je razvoj specifi~nih antitela protiv malog antigena. Vitamin D je tako|e jako hidrofoban molekul predominantno vezan za vitamin D vezu ju}i protein (DBP). Stoga postoji verovatno}a da na imuno odre|ivanja u razli~itom stepenu mo`e da uti~e koncentracija DBP. Uvo|enje LC-MS/MS u...

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The C-3 Epimer of 25-Hydroxyvitamin D₃Is Present in Adult Serum

Cited by 161 publications

References 18 publications

MANAGEMENT OF ENDOCRINE DISEASE: Value and limitations of assessing vitamin D nutritional status and advised levels of vitamin D supplementation

MANAGEMENT OF ENDOCRINE DISEASE: Value and limitations of assessing vitamin D nutritional status and advised levels of vitamin D supplementation

Mass Spectrometric Profiling of Vitamin D Metabolites beyond 25-Hydroxyvitamin D

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

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The C-3 Epimer of 25-Hydroxyvitamin D3Is Present in Adult Serum

Cited by 161 publications

References 18 publications

MANAGEMENT OF ENDOCRINE DISEASE: Value and limitations of assessing vitamin D nutritional status and advised levels of vitamin D supplementation

MANAGEMENT OF ENDOCRINE DISEASE: Value and limitations of assessing vitamin D nutritional status and advised levels of vitamin D supplementation

Mass Spectrometric Profiling of Vitamin D Metabolites beyond 25-Hydroxyvitamin D

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

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The C-3 Epimer of 25-Hydroxyvitamin D₃Is Present in Adult Serum